INTERACTION OF RECOMBINANT TATA-BINDING PROTEIN WITH MAMMALS GENES PROMOTER TATA



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Abstract

Quantitative characteristics of interaction recombinant TATA-binding protein (TBP) with oligonucleotides identical to natural TATA-containing promoter region genes of mammals are received. In particular, new experimental data about the importance guanine in 8-th position of the TATA-element for affinity to TBP are received. The experimental data, testifying that raised maintenance G and С nucleotides in flanks of TATA-element does the contribution to affinity to TBP are received.

About the authors

Ludmila K Savinkova

Institute of Cytology and Genetics SB RAS, Novosibirsk, Novosibirsk Oblast, RF

Email: savinkl@mail.ru

Irina A Drachkova

Institute of Cytology and Genetics SB RAS, Novosibirsk, Novosibirsk Oblast, RF

Michail P Ponomarenko

Institute of Cytology and Genetics SB RAS, Novosibirsk, Novosibirsk Oblast, RF

Marina V Lysova

Institute of Cytology and Genetics SB RAS, Novosibirsk, Novosibirsk Oblast, RF

Tatiana V Arshinova

Institute of Cytology and Genetics SB RAS, Novosibirsk, Novosibirsk Oblast, RF

Nikolay A Kolchanov

Institute of Cytology and Genetics SB RAS, Novosibirsk, Novosibirsk Oblast, RF

Email: kol@bionet.nsc.ru

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Copyright (c) 2007 Savinkova L.K., Drachkova I.A., Ponomarenko M.P., Lysova M.V., Arshinova T.V., Kolchanov N.A.

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