Double Mutant Analysis of Sequential Functioning of Pea (Pisum sativum L.) Genes: Sym13, Sym33 and Sym40 During Symbiotic Nodule Development



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Abstract

Two double mutants carrying pea symbiotic gene pairs sym13, sym40 and sym33, sym40, respectively, were constructed using single mutants blocked at different nodule developmental stages: E135f (sym13), SGEFix–‑1 (sym40) and SGEFix–‑2 (sym33). The epistasis of the mutant allele sym40 over the mutant allele sym13 and sym33 over sym40 was shown with respect to nodule histological and ultrastructural organisation. Thus, the proposed earlier sequential functioning of genes during infection process: Sym33→Sym40→Sym13 has been confirmed. 

About the authors

Viktor E Tsyganov

All-Russia Research Institute for Agricultural Microbiology, Saint-Petersburg, RF

Email: viktor_tsyganov@arriam.spb.ru

Elena V Seliverstova

All-Russia Research Institute for Agricultural Microbiology, Saint-Petersburg, RF

Email: elena306@yandex.ru 196608, Санкт-Петербург, Пушкин-8, ш. Подбельского, д. 3

Vera A Voroshilova

Contract Drug Development Company “PSI”, St. Petersburg, RF

Email: vera.voroshilova@psi-cro.com

Anna V Tsyganova

All-Russia Research Institute for Agricultural Microbiology, Saint-Petersburg, RF

Email: anna_khodorenko@arriam.spb.ru Podbelskiy Ch., 3, Saint-Petersburg, Pushkin-8, 196608, Russ

Zlata B Pavlova

All-Russia Research Institute for Agricultural Microbiology, Saint-Petersburg, RF

Email: zlata.pavlova@psi-cro.com 196608, Санкт-Петербург, Пушкин-8, ш. Подбельского, д. 3

Vladimir K Lebsky

All-Russia Research Institute for Agricultural Microbiology, Saint-Petersburg, RF

Email: lebsky04@cibnor.mx

Aleksey U Borisov

All-Russia Research Institute for Agricultural Microbiology, Saint-Petersburg, RF

Email: ayborisov@yandex.ru

Igor A Tikhonovich

All-Russia Research Institute for Agricultural Microbiology, Saint-Petersburg, RF

Email: arriam@arriam.spb.ru. contact@arriam.spb.ru Podbelskiy Ch., 3, Saint-Petersburg, Pushkin-8

Nicholas Brewin

The John Innes Centre, Norwich, UK

Email: nick.brewin@bbsrc.ac.uk NR4 7UH, Colney Lane, Norwich, United Kingdom

References

  1. Цыганова А. В., Цыганов В. Е., Финдли К. К. и др., 2009. Распределение арабиногалактанпротеинов‑эк- стензинов в клубеньках мутантов гороха (Pisum sativum L.) с нарушениями в развитии инфекционной нити // Цитология. Т. 51. № 1. С. 53-62.
  2. Avery L., Wasserman S., 1992. Ordering gene function: the interpretation of epistasis in regulatory hierarchies // Trends Genet. Vol. 8. P. 312-316.
  3. Bhatia C. R., Nichterlein K., Maluszynski M., 2001. Mutations affecting nodulation in grain legumes and their potential in sustainable cropping systems // Euphytica. Vol. 120. P. 415-432.
  4. Borisov A. Y., Morzhina E. V., Kulikova O. A. et al., 1992. New symbiotic mutants of pea (Pisum sativum L.) affecting either nodule initiation or symbiosome development // Symbiosis. Vol. 14. P. 297-313.
  5. Borisov A. Y., Rozov S. M., Tsyganov V. E. et al., 1997. Sequential functioning of Sym‑13 and Sym‑31, two genes affecting symbiosome development in root nodules of pea (Pisum sativum L.) // Mol. Gen. Genet. Vol. 254. P. 592-598.
  6. Borisov A. Y., Danilova T. N., Koroleva T. A. et al., 2004. Pea (Pisum sativum L.) regulatory genes controlling development of nitrogen‑fixing nodule and arbuscular mycorrhiza: fundamentals and application // Biologia. Vol. 59, Suppl. P. 137-144.
  7. Brewin N. J., 2004. Plant cell wall remodelling in the Rhizobium‑legume symbiosis // Critic. Rev. Plant Sci. Vol. 23. P. 293-316. 8. Caetano‑Anollés G., Gresshoff P. M., 1991. Plant genetic control of nodulation // Ann. Rev. Microb. Vol. 45. P. 345-382.
  8. Guinel F. C., Geil R. D., 2002. A model for the development of the rhizobial and arbuscular mycorrhizal symbiosis in legumes and its use to understand the roles of ethylene in the establishment of these two symbiosis // Can. J. Bot. Vol. 80. P. 695-720.
  9. Kneen B. E., LaRue T. A., Hirsch A. M. et al., 1990. sym13 - a gene conditioning ineffective nodulation in Pisum sativum // Plant Physiol. Vol. 94. P. 899-905.
  10. Koornnef M., Alonso‑Blanco C., Peeters A. J. M. et al., 1998a. Genetic control of flowering time in Arabidopsis // Annu. Rev. Plant Physiol. Plant Mol. Biol. Vol. 49. P. 345-370.
  11. Koornnef M., Alonso‑Blanco C., de Vries H. B. et al., 1998b. Genetic interactions among late‑flowering mutants in Arabidopsis // Genetics. Vol. 148. P. 885-892.
  12. Kosterin O. E., Rozov S. M., 1993. Mapping of the new mutation blb and the problem of integrity of linkage group I // Pisum Genet. Vol. 25. P. 27-31.
  13. Morzhina E. V., Tsyganov V. E., Borisov A. Y. et al., 2000. Four developmental stages identified by genetic dissection of pea (Pisum sativum L.) root nodule morphogenesis // Plant Sci. Vol. 155. P. 75-83.
  14. Oldroyd G. E. D., Downie J. A., 2004. Calcium, kinases and nodulation signalling in legumes // Mol. Cell Biol. Vol. 5. P. 566-576.
  15. Oldroyd G. E. D., Downie J. A., 2008. Coordinating nodule morphogenesis with rhizobial infection in legumes // Annu. Rev. Plant Biol. Vol. 59. P. 519-546.
  16. Parker J. S., Cavell A. C., Dolan L. et al., 2000. Genetic interactions during root hair morphogenesis in Arabidopsis // Plant Cell. Vol. 12. P. 1961-1974.
  17. Rathbun, E. A., Naldrett, M. J., Brewin N. J., 2002. Identification of a family of extensin‑like glycoproteins in the lumen of Rhizobium‑induced infection threads in pea root nodules // Mol. Plant‑Microbe Interact. Vol. 15. P. 350-359.
  18. Safronova V. I., Novikova N. I., 1996. Comparison of two methods for root nodule bacteria preservation: lyophilization and liquid nitrogen freezing // J. Microbiol. Methods. Vol. 24. P. 231-237.
  19. Tsyganov V. E., Morzhina E. V., Stefanov S. Y. et al., 1998. The pea (Pisum sativum L.) genes sym33 and sym40 control infection thread formation and root nodule functioning // Mol. Gen. Genet. Vol. 259. P. 491-503.
  20. Tsyganov V. E., Voroshilova V. A., Priefer U. B. et al., 2002. Genetic dissection of the initiation of the infection process and nodule tissue development in the Rhizobium‑pea (Pisum sativum L.) symbiosis // Ann. Bot. (Lond.). Vol. 89. P. 357-366.
  21. Tsyganov V. E., Voroshilova V. A., Herrera‑Cervera J. A. et al., 2003. Developmental down‑regulation of rhizobial genes as a function of symbiosome differentiation in symbiotic root nodules of Pisum sativum L. // New Phytol. Vol. 159. P. 521-530.
  22. Voroshilova V. A., Boesten B., Tsyganov V. E. et al., 2001. Effect of mutations in Pisum sativum L. genes (sym13, sym31, sym33, sym40) blocking different stages of nodule development on the expression of late symbiotic genes in Rhizobium leguminosarum bv. viciae // Mol. Plant‑Microbe Interact. Vol. 14. P. 471- 476.
  23. Walker S. A., Viprey V., Downie J. A., 2000. Dissection of nodulation signaling using pea mutants defective for calcium spiking induced by Nod factors and chitin oligomers // PNAS. Vol. 97. P. 13413-13418.
  24. Wang T. L., Wood, E. A., Brewin N. J., 1982. Growth regulators, Rhizobium and nodulation of peas // Planta. Vol. 155. P. 345-349.

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Copyright (c) 2010 Tsyganov V.E., Seliverstova E.V., Voroshilova V.A., Tsyganova A.V., Pavlova Z.B., Lebsky V.K., Borisov A.U., Tikhonovich I.A., Brewin N.

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