The role of DNA polymerase alpha in the control of mutagenesis in Saccharomyces cerevisiae cells starved for nutrients



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Abstract

In nature, microorganisms experience numerous environmental stresses and generally grow poorly most of the time. In the last two decades it has become evident that mutations arise not only in actively dividing cells but also in nonreplicating or slowly replicating cells starved for nutrients. In yeast, precise base selection and proofreading by replicative DNA polymerases δ and ε keep starvation-associated mutagenesis (SAM) at basal levels. Less is known about the role of replicative DNA polymerase α (Pol α). Here we provide evidence that Pol α is involved in the control of SAM in yeast cells starved for adenine by participation in sporadic replication and/or DNA repair under these conditions. 

About the authors

Nora Babudri

University of Perugia, Perugia, Italy

Email: babudri@upihg.it

Alessandro Achilli

University of Pavia, Pavia, Italy

Email: achilli@unipg.it.

Chiara Martinelli

University of Pavia, Pavia, Italy

Email: chiaramartinelli@hotmail.it.

Elizabeth Moore

University of Nebraska, Linkoln, NE, USA

Email: eamoore@unmc.edu.

Hovirag Lancioni

University of Pavia, Pavia, Italy

Email: lancioni@kataweb.com

Yuri I Pavlov

Eppley Institute for Research in Cancer and Allied Diseases, Omaha, NE, USA

Email: ypavlov@unmc.edu

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Copyright (c) 2011 Babudri N., Achilli A., Martinelli C., Moore E., Lancioni H., Pavlov Y.I.

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