COMBINED PRESERVATIVE PROPERTIES USED TO FREEZE PLATELET PRESERVATIVES

Aim. To determine the properties of a new cryopreservative based on hexamethylentetraoxyethylurea (HMBTOEU) and dimethylacetamide (DMAC) and its effect on functional platelet ability. Materials and methods. The effect of different variants of cryopreservative on the platelet function was analyzed. Results. Osmolarity of the studied solutions was from 184 to 750 mosm/l. When the solution components’ concentration increased their osmolarity grew. The acidity index (pH) of the prepared forms of cryopreservative was 5,6–6,0, pH of native platelet preservatives (PP) – 7,32±0,12 and their osmolarity – 309±13,0 mosm/l. After cell suspension was mixed with cryophylactic solutions, pH of platelet preservatives decreased from 7,0 to 6,2, osmolarity ranged from 246,2±8,62 to 541,0±23,0 mosm/l; 92,9–96,2% of the basic amount of cells was preserved and their functional activity was registered at the level of 61,5–84% (of the corresponding native PP indices) Conclusion. The composition of cold-protective solution determines physic-chemical properties of cryopreservative itself (pH, osmolarity) as well as the degree of changes in morphofunctional activity at the stage of PP exposition with cryopreservative.

Cryopreservation, platelet preservative, pH, osmolarity, adhesion, aggregation, response to hypotonic shock