Vol 14, No 4 (2016)
- Year: 2016
- Articles: 9
- URL: https://journals.eco-vector.com/RCF/issue/view/343
- DOI: https://doi.org/10.17816/RCF144
Articles
Effect of cholinotropic agents to change the content of oxygen triplet forms of liver tissue and the ability of the liver homogenate to produce active oxygen in rat cooling
Abstract
The method of polarographic analysis of elements in the tissue solutions of living organisms, and the method of checking the ability of the liver homogenate to produce active forms of oxygen are among the methods for assessing the content of triplet forms of oxygen in the tissue and the ability of the tissue homogenate to initiate lipid peroxidation (POL) after a cold load and administration of cholinotropic agents to rats. In the study, the content of triplet form of oxygen of liver homogenate and the ability of the liver homogenate to produce active oxygen in the period of 3 hour and 5 days cooling of experimental animals were determined. The effect of cholinotropic agent accumulating the endogenous acetylcholine in liver tissue, pharmacological agents that stimulate and block the work of muscarine-sensitive cholinoreactive structures of hepatocyte plasmatic membranes with the assessment of their impact on the content of triplet forms of oxygen, the ability of the liver homogenate to produce active oxygen in supercooling of animals were investigated. Neostigmine on the background of 3 hours cold exposure led to a decrease in the content of triplet forms of oxygen but increased the ability of the liver homogenate to produce active forms of oxygen. Pilocarpine and atropine on the background of cooling animals in 5 days period caused the manifestation of reciprocity as in 3 minutes, so as in 30 minutes of experiment for the determination of triplet forms of oxygen of the liver tissue. Besides pilocarpine and atropine reduced the ability of liver tissue to produce active forms of oxygen. The obtained results indicate the change in the content of triplet forms of oxygen in the liver tissue when pilocarpine and atropine administrated to animals on the backdrop of 5 days cooling. Additionally the results show that the injection of neostigmine to animals on the background of 3 hours cooling promotes the increase of active forms of oxygen.
Search for the new anti-inflammatory agents based on glycosylated polypeptide complex extracted from sea urchins Strongylocentrotus droebachiensis
Abstract
We performed the study of the anti-inflammatory activity of glycosylated polypeptide complex (GPC) extracted from sea urchins Strongylocentrotus droebachiensis in the model of acute bronchitis induced by endotracheal administration of 1% formalin solution in male Wistar rats. Animals of experimental groups were inhaled by GPC solution at 25, 50 and 100 mcg/kg, control animals – by the reference drug ambroxol at 3.6 mg/kg once daily during 7 days starting from the day of pathology induction. 24 hours after pathology induction GPC at 100 mcg/kg reduced the total leukocyte count in the bronchoalveolar lavage fluid by 34%, after 48 hours – by 61% compared with control animal group (р ≤ 0.05). If the normal number of goblet cells per 1 mm bronchial mucosa was 33.7 ± 1.7 and after formalin exposure – 67.5 ± 1.8, then after a course administration of polypeptide complex (7 days) at 100 mcg/kg – 55.7 ± 2.7. By the results of morphology and histology study we determined that mononuclear infiltration of bronchial submucosa layer by lymphocytes and macrophages, neutrophil leukocytes of mucosa and submucosa was significantly less than in control animal group (р < 0.05) affected by formalin without any pharmacological protection. Upon the results of the study, we determined the anti-inflammatory activity of glycosylated polypeptide complex at 25, 50 and 100 mcg/kg, which was not inferior to the similar activity of ambroxol (3.6 mg/kg).
Cyclo-prolylalanine: the neuroprotective and antioxidative properties on the model of acute cerebral disease in rats
Abstract
Astressin, an antagonist of CRF receptors, reduces anxiety and fobial states in rats reared in social isolation conditions
Abstract
Vital stress causes long-lasting behavioral disorders and lipid metabolism deviations in female rats
Abstract
Summary. Female rats after the vital stress caused by experience of the circumstances of the death of a partner from the actions of the predator showed long-lasting behavioral disturbances, such as decreased locomotor activity, exploratory activity, increased anxiety and aggression. Indicators of lipid metabolism of rats were altered in comparison with control at different time after exposure. These changes through two months later a single stress and right after second psychogenic trauma were characterized by reduced total cholesterol and cholesterol of high density lipoproteins in serum and increased triglycerides in the liver. The severity of behavioral disorders and deviations from the control values of indicators of lipid metabolism in female rats depended on the phases of the estrous cycle. The revealed deviations are discussed in terms of post-traumatic stress disorder.