Molecular-genetic characterization of the human non-hypervariable GC-rich minisatellite UPS29 of gene CENTB5



Cite item

Full Text

Abstract

Human minisatellite UPS29 localized in one of CENTB5 introns was studied in silico and using molecular genetic analysis. For the first time there were revealed seven UPS29 alleles which contained 6-24 repeated units. Allele consisting of 17 repeats was prevailed (91,5 %). Frequency of other alleles varied from 0,29 % to 4,39 %. UPS29 heterozygosity was 12,3 %. Minisatellite UPS29 was classified as low polymorphic and non hypervariable.

About the authors

Irina O Suchkova

Institute of Experimental Medicine, St. Petersburg, RF

Email: irsuchkova@mail.ru

Daria M Shubina

Institute of Experimental Medicine, St. Petersburg, RF

Email: iem@iem.spb.ru

Ludmila K Sasina

Institute of Experimental Medicine, St. Petersburg, RF

Email: sassina2001@hotmail.com

Natalia O Slominska

Institute of Experimental Medicine, St. Petersburg, RF

Vadim B Vasilyev

Institute of Experimental Medicine, St. Petersburg, RF

Natalia V Alenina, 

Max Delbr?ck Center for Molecular Medicine, Berlin, Germany

Mikhael F Bader

Max Delbr?ck Center for Molecular Medicine, Berlin, Germany

Eugene L Patkin

Institute of Experimental Medicine, St. Petersburg, RF

Email: elp44@mail.ru

References

  1. Айала Ф. Современная генетика. В 3-х т., Т.3.: пер. с англ./Айала Ф., Кайгер Дж.; пер. с англ. Базыки-на А. Д. -М.: Мир, 1988. -335 с.
  2. Аксенова М. Г. Аллельный полиморфизм гена переносчика дофамина в группах больных эндогенными психозами. Связь с патологическими синдромами/Аксенова М. Г., Голимбет В. Е., Алфимова М. В., Носиков В. В.//Молекулярная биология. -2000. -Т. 34, № 4. -С. 696-700.
  3. Ивантер Э. В. Основы биометрии: введение в статистический анализ биологических явлений и процессов/Ивантер Э. В., Коросов А. В. -Петрозаводск.: Изд-во Петрозаводского гос. университета, 1992. -168 с.
  4. Кайданов Л. З. Генетика популяций//Кайданов Л. З. -М.: Высш. шк., 1996. -320 с.
  5. Паткин Е. Л. Сателлитные ДНК и болезни -возможные механизмы. Нестабильность минисателлитов/Паткин Е. Л., Гайцхоки В. С.//Генетика. -2000. -Т. 36, № 9. -С. 1189-1194.
  6. Patkin E. L., Gaitskhoki V.S. Satellite DNAs and diseases -possible mechanisms: minisatellite instability//Rus. J. Genetics. -2000. -Vol. 36, N 9. -P. 989-993.).
  7. Плохинский Н.А. Биометрии//Плохинский Н. А. -М.: Изд-во МГУ, 1970. -368 с.
  8. Andreassen R. De novo mutation and allelic diversity at minisatellite locus D7S22 investigated by allele-specific four-state MVR-PCR analysis/Andreassen R., Olaisen B.//Hum. Mol. Genet. -1998. -Vol. 7. -P. 2113-2120.
  9. Benson G. Tandem repeats finder: a program to analyze DNA sequences/Benson G.//Nucl. Acid Res. -1999. -Vol. 27. -P. 573-580.
  10. Birth and evolution history of a human minisatellite/Boan F., Blanco M. G., Quinteiro J. [et al.]//Mol. Biol. Evol. -2004. -Vol. 21. -P. 228-235.
  11. Boan F. Molecular characterization of a new human minisatellite that is able to form single-stranded loops in vitro and recognized by nuclear proteins/Boan F., Gonzalez A. I., Rodriguez J. M., Gomez-Marquez J.//FEBS Letters. -1997. -Vol. 418. -P. 251-257.
  12. Boan F. A non-hypervariable human minisatellite strongly stimulates in vitro intramolecular homologous recombination/Boan F., Rodriguez J. M., Gomez-Marquez J.//J. Mol. Biol. -1998. -Vol. 278. -P. 499-505.
  13. Brukner I. Sequence dependent bending propensity of DNA as revealed by DNAse I: Parameters for trinucleotides/Brukner I., Sanchez R., Suck D., Pongor S.//EMBO J. -1995. -Vol. 14. -P. 1812-1818.
  14. Buard J. Complex recombination events att the hypetmutable minisatellite CEB1 (D2S90)/Buard J.,Vergnaud G.//EMBO J. -1994. -Vol. 13. -P. 3203-3210.
  15. Denoeud F. Predicting human minisatellite polymorphism/Denoeud F., Vergnaud G., Benson G.//Genome Res. -2003. -Vol. 13. -P. 856-867.
  16. Goodsell D. S. Bending and curvature calculations in B-DNA/Goodsell D. S., Dickerson R. E.//Nucl. Acids Res. -1994. -Vol. 22. -P. 5497-5503.
  17. Human chromosomal fragile site FRA16B is amplified AT-rich minisatellite repeat/Yu S., Mangelsdorf M., Hewett D. [et al. ]//Cell. -1997. -Vol. 88. -P. 367-374.
  18. Jobling M. A. Hypervariable digital DNA codes for human paternal lineages: MVR-PCR at the Y-specific minisatellite, MSY1 (DYF155S1)/Jobling M. A., Biuzekri N., Taylor P. G.//Hum. Mol. Genet. -1998. -Vol. 7. -P. 643-653.
  19. MacKenzie A. A serotonin transporter gene intron 2 polymorphic region, correlated with affective disoders, has allele-dependent differential enhancerlike properties in the mouse embryo/MacKenzie A.,Quinn J.//Proc. Natl. Acad. Sci. USA. -1999. -Vol. 96. -P. 15251 -15255.
  20. Molecular basis for expression of common and rare fragile sites/Zlotorynski E., Rahat A., Skaug J. [et al.]//Mol. Cell Biol. -2003. -Vol. 23. -P. 7143-7151.
  21. Ovarian canser risk in BRCA1 carriers is modified by the HRAS1 variable number of tandem repeat (VNTR) locus/Phelan C. M., Rebbeck T. R., Weber B. L. [et al.]//Nat. Genet. -1996. -Vol. 12. -P. 309-311.
  22. Papadakis E. N. Genetic analysis of H-ras intron-1 polymorphic and variabletandem repeatregions in human breast cancer/Papadakis E. N., Dokianakis D. N.,Spandidos D. A.//Int J. Biol. Markers. -2003. -Vol. 18. -P. 195-199.
  23. Structure of Chi hotspots of generalized recombination/Smith G. R., Kunes S. M., Schultz D. W. [et al.]//Cell. -1981. -Vol. 24. -P. 429-436.
  24. The use synthetic tandem repeats to isolate new VNTR loci: cloning of a human hypermutable sequence/Vergnaud G., Moriat D., Apiou F. [et al.]//Genomics. -1991. -Vol. 11. -P. 135-144.
  25. Vergnaud G. Minisatelletes: mutability and genome architecture/Vergnaud G., Denoeud F.//Genet. Res. -2000. -Vol. 10. -P. 899-907.
  26. Weinreb A. Site of uneqiual sister chromatid exchange contains a potential Z-DNA-froming tract/Weinreb A.,Katzenberg D. R.//Proc. Natl. Acad. Sci. USA. -1988. -Vol. 85. -P. 529-533.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2007 Suchkova I.O., Shubina D.M., Sasina L.K., Slominska N.O., Vasilyev V.B., Alenina,  N.V., Bader M.F., Patkin E.L.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС 77 - 65617 от 04.05.2016.


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies