GENETIC ESTIMATION OF SNAIL LYMNAEA STAGNALIS POPULATIONS FROM REGIONS WITH DIFFERENT ANTHROPOGENIC LOAD AS A FIRST STEP IN GENETIC MONITORING



Cite item

Full Text

Abstract

The paper deals with carrying out molecular-genetic typing of populations of snail Lymnaea stagnalis from regions with a different ecological load (the lake Perstok and zatoka of the river Pripyat, Belarus). By means of interpopulation analysis of frequencies of RAPD-fragments, frequencies of zero alleles of RAPD-loci and genetic similarity index we established that these populations are genetically identical because there are no significant distinctions of genetic variability neither between individuals within a population, nor between populations. Results of the cluster analysis and the population analysis also have confirmed that populations of snails are genetically closely related. However the snail population of the lake Perstok is genetically more homogeneous.

About the authors

Oksana Yu Koneva

Institute of Genetics and Cytology of the NASB, Minsk, Republic of Belarus

Email: koneva@tut.by

References

  1. Булат С. А., Мироненко Н. В., Жолкевич Ю. Г. 1995. Генетическая структура почвенной популяции гриба Fusarium oxysporum Schlechtend.: Fr.: Молекулярная реидентификация вида и генетическая дифференциация изолятов методом полимеразной цепной реакции с универсальными праймерами (УП-ПЦР)//Генетика. Т. 31, № 3, С. 315-323.
  2. Голубев А. П., Сикорский В. Г., Калинин В. Н. и др., 2007. Динамика радиоактивного загрязнения экосистем разнотипных водоемов белорусского сектора зоны отчуждения Чернобыльской АЭС//Радиационная биология. Радиоэкология. T. 47, № 3. C. 322-332.
  3. Дромашко С. Е., Конева О. Ю. 2010. Генетическая паспортизация пород белорусского карпа//Наука и инновации. № 11 (93). С. 36-40.
  4. Хедрик Ф., 2003. Генетика популяций. М.: Техносфера. 592 с.
  5. Хрусталёва А. М. 2007. Генетическая дифференциация нерки (Оncorhynchus Nerka) азиатских стад: Автореф. дис. … канд. биол. наук. М., 25 с.
  6. Abdel-Hamid A.-Z., Rawi S. M., Arafa A. F. 2006. Identification of a genetic marker associated with the resistance to Schistosoma mansoni infection using random amplified polymorphic DNA analysis//Memórias do Instituto Oswaldo Cruz. Vol. 101. No 8. P. 863-868.
  7. Aranishi F., Okimoto T. 2004. Genetic relationship between cultured populations of Pacific oyster revealed by RAPD analysis//Journal of Applied Genetics. Vol. 45. No 4. P. 435-443.
  8. Elo K., Ivanoff S., Vuorinen J. A., Piironen J. 1997. Inheritance of RAPD markers and detection of interspecific hybridization with brown trout and Atlantic salmon//Aquaculture. Vol. 152. P. 55-65.
  9. Golubev A. P., Afonin V., Maksimova S., Androsov V. 2005. The current state of pond snail Lymnaea stagnalis populations from water reservoirs of the Chernobyl nuclear accident zone//Radioprotection. Vol. 40. Suppl. 1. P. S511-S517.
  10. Holthausen R., Czaplewski R. L., DeLorenzo D. et al., 2005. Strategies for monitoring terrestrial animals and habitats. General Technical Report RMRS-GTR‑161. Fort Collins, CO: U. S. Department of Agriculture, Forest Service, Rocky Mountain Research Station. 34 p.
  11. Kurtz J. C., Jackson L. E., Fisher W. S. 2001. Strategies for evaluating indicators based on guidelines from the Environmental Protection Agency's Office of Research and Development//Ecological Indicators. Vol. 1. P. 49-60.
  12. Manly B. F. J. 1985. The statistics of natural selection on animal populations. London: Chapman & Hall. 440 p.
  13. Мonitoring and indicators of forest biodiversity in Europe -form ideas to operationality. 2004/Marchetti M. (ed.). EFI Proceedings. No. 51. 526 p.
  14. Morin C., Breuil C., Bernier L. 2004. Genetic Variability and Structure of Canadian Populations of the Sapstain Fungus Ceratocystis resinifera//Phytopathology. Vol. 94. No. 12. P. 1323-1330.
  15. Nei M., Li W.‑H. 1979. Genetics Mathematical model for studying genetic variation in terms of restriction endonucleases//Proceedings of the National Academy of Sciences of USA. Vol. 76. No 10, P. 5269-5273.
  16. Nei M. 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals//Genetics. Vol. 89. P. 583-590.
  17. Nei M. 1987. Molecular Evolutionary Genetics. New York: Columbia University Press. 512 p.
  18. Saitoh K. 1998. Genetic variation and local differentiation in the Pacific cod Gadus macrocephalus around Japan revealed by mtDNA and RAPD markers//Fisheries Science. Vol. 64. P. 673-679.
  19. Schwartz M. K., Luikart G., Waples R. S. 2007. Genetic monitoring as a promising tool for conservation and management//Trends in Ecology and Evolution. Vol. 22. No 1. P. 25-33.
  20. Slatkin M., Barton N. H., 1989. A comparison of three indirect methods for estimatingaverage levels of gene flow//Evolution. Vol. 43. P. 1349-1368.
  21. Weir B. S., Reynolds J., Dodds K. G. 1990. The variance of sample heterozygosity//Theoretical Population Biology. Vol. 37. No 1. P. 235-253.
  22. Welsh J., McClelland M. 1990. Fingerprinting genomes using PCR with arbitrary primers//Nucleic Acids Research. Vol. 18. No 24. P. 7213-7218.
  23. Williams J. G. K., Kubelik A. R., Livak K. J. et al., 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers//Nucleic Acids Research, Vol. 18, No 22. P. 6531-6535.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2012 Koneva O.Y.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС 77 - 65617 от 04.05.2016.


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies