Lux-biosensors: screening biologically active compounds for genotoxicity

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Abstract


To study the ability of metal salts and pharmacologically active drugs to induce the oxidative stress and SOS response in bacteria, a set of lux-biosensors was used. The sensors were based on three E. coli strains carrying recombinant plasmids with lux-operon fused to the promoters of SoxS (superoxide dismutase), KatG (catalase-peroxidase) and ColD (colicin D) genes. The created biosensors were used to analyze the activity of 47 substances, with 16 of them identified to induce SOS-response and 6 - to induce oxidative stress. The results observed were compared to the previously published data on the mutagenic activity of the same 47 substances evaluated using Ames test. The comparison had shown full coincidence for 42 from 47 substances analyzed. We discuss the possibility to use the lux-based biosensors for the screening of the genetic activity of chemical compounds.

Elena V Igonina

Author for correspondence.
iev555@ya.ru
Vavilov Institute of General Genetics, Russian Academy of Sciences
Russian Federation

Ph.D., research worker, Lab. of ecological genetics

Mariya V Marsova

masha_marsova@mail.ru
Vavilov Institute of General Genetics, Russian Academy of Sciences
Russian Federation

junior research worker, Laboratory of Bacterial Genetics

Serikbai K Abilev

abilev@vigg.ru
Vavilov Institute of General Genetics, Russian Academy of Sciences
Russian Federation

Doctor of Biological Sciences, Professor, Deputy Directors

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Supplementary files

Supplementary Files Action
1. Fig. 1. Luminescence of E. coli MG1655 (pColD–lux) induced by mitomycin C. The exposure time – 50 minutes View (29KB) Indexing metadata
2. Fig. 2. Luminescence of E. coli MG1655 (pKatG–lux) induced by hydrogen peroxide. Exposure time – 25 minutes View (31KB) Indexing metadata
3. Fig. 3. Luminescence of E. coli MG1655 (pSoxS–lux) induced by paraquat. Exposure time – 30 minutes View (30KB) Indexing metadata

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