DNA-cytometric characteristics of primary soft tissue sarcomas

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Abstract


Aim. Determination of deoxyribonucleic acid (DNA) content and cell distribution in mytotic cycle phases in the groups of patients with primary soft tissue sarcomas.

Methods. Study material was the tumours of patients with primary soft tissue sarcomas. Study methods included histological, DNA cytometric and statistical methods.

Results. Differences in proliferative activity and proliferation index in primary sarcomas depending on the tumor differentiation grade and disease stage were revealed. Differences in the numbers of diploid, aneuploid and polyploid cells depending on the cell cycle phases were determined in each group and between groups. Depending on the differentiation grade of soft tissue sarcoma, parameters of the cell cycle in soft tissue sarcomas were as follows: well-differentiated tumors (G1) were diploid in 100% of cases, and moderately differentiated tumors (G2) were aneuploid in 50% of cases. Poorly differentiated tumors (G3) were characterized by significantly higher content of aneuploid cells, in comparison with G2 tumors (33.1±5.2 and 16.5±6.8, respectively). Analysis of kinetic parameters of cell cycle demonstrated decreased proportion of cells in G1/G0 cell cycle phase from G1 to G3, accompanied by a statistically significant increase of the proportion of cells in S phase (p ˂0.05).

Conclusion. DNA cytometric study of cell cycle parameters demonstrated high biological potential of primary soft tissue sarcomas; high malignant potential can probably be determined by two parameters: proportion of the cells in G2+М phase and cell loss factor.


I A Novikova

Author for correspondence.
iftrnioi@yandex.ru
Rostov Scientific Research Institute of Oncology Rostov-on-Don, Russia

E M Nepomnyashchaya

iftrnioi@yandex.ru
Rostov Scientific Research Institute of Oncology Rostov-on-Don, Russia

E P Ul’yanova

iftrnioi@yandex.ru
Rostov Scientific Research Institute of Oncology Rostov-on-Don, Russia

O N Selyutina

iftrnioi@yandex.ru
Rostov Scientific Research Institute of Oncology Rostov-on-Don, Russia

T A Aliev

iftrnioi@yandex.ru
Rostov Scientific Research Institute of Oncology Rostov-on-Don, Russia

L N Vashchenko

iftrnioi@yandex.ru
Rostov Scientific Research Institute of Oncology Rostov-on-Don, Russia

T V Ausheva

iftrnioi@yandex.ru
Rostov Scientific Research Institute of Oncology Rostov-on-Don, Russia

E I Zolotareva

iftrnioi@yandex.ru
Rostov Scientific Research Institute of Oncology Rostov-on-Don, Russia

  • Fletcher C.D.M., Bridge J.A., Hogendoorn P.C.W., Mertens F. World Health Organization Classification of tumours of soft tissue and bone. 4th ed. Lyon: IARC Press. 2013; 80 p.
  • Patrikidou A., Domont J., Cioffi A., Le Cesne A. Treating soft tissue sarcomas with adjuvant chemotherapy. Curr. Treat. Options Oncol. 2011; 12 (1): 21-31. doi: 10.1007/s11864-011-0145-5.
  • Coindre J.M. New WHO classification of tumours of soft tissue and bone. Annales de pathologie. 2012; 32: 115-116.
  • Базанов К.В. Исследование скорости роста рецидивных и метастатических сарком мягких тканей. Соврем. пробл. науки и образования. 2014; 6: 1227.
  • Колобов А.В., Полежаев А.А. Влияние случайной подвижности злокачественных клеток на устойчивость фронта опухоли. Компьютерные исследования и моделирование. 2009; 1 (2): 225-232.
  • Кит О.И., Новикова И.А., Бахтин А.В. и др. Первый опыт детекции циркулирующих опухолевых клеток в периферической крови. Международ. ж. эксперим. образования. 2013; 11: 37-39.
  • Неродо Г.А., Новикова И.А., Никитина В.П. и др. Клиническое значение ДНК проточной цитометрии при раке яичников. Соврем. пробл. науки и образования. 2016; 3: 35.
  • Кумар В., Аббас А.К., Фаусто Н., Астер Дж.К. Основы патологии заболеваний по Роббинсу и Котрану. Пер с англ. Под ред. Е.А. Коган. В 3 т. М.: Логосфера. 2014; 1 (главы 1-10): 312-313.
  • Николаева Т.Г., Добрынин Я.В. Плоидность опухолевых клеток - важный прогностический фактор при некоторых злокачественных эпителиальных новообразованиях человека (собственные и литературные данные). Вестн. РОНЦ им. Н.Н. Блохина РАМН. 2006; 17 (1): 29-36.

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© 2017 Novikova I.A., Nepomnyashchaya E.M., Ul’yanova E.P., Selyutina O.N., Aliev T.A., Vashchenko L.N., Ausheva T.V., Zolotareva E.I.

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