Species spectrum of microflora of upper digestive tract mucosa and its effect on the development of pathology in children

Cover Page


Aim. To study microbial landscape of duodenal mucosa and bile samples from children with disorders of the upper digestive tract.

Methods. The study material included 69 biopsy samples of duodenal mucosa obtained during upper endoscopy and bile samples (106 samples) obtained at fractional duodenal sounding of 106 children. An expanded set of growth media (5% blood agar, Endo’s medium, Ploskireva’s medium and alkaline bile agar) was used followed by biochemical identification, that allows revealing a wide range of contamination with aerobic and facultative anaerobic microorganisms.

Results. It was found that in children with isolated forms of chronic gastroduodenitis and functional gallbladder disorders the frequency of H. pylori detection was significantly higher compared to other children who have organic desease of biliary system in the form of chronic сholecystitis.

Conclusion. Infection is the trigger mechanism for the development of combined pathology of the upper digestive tract and the biliary system; in organic cholepathies mixed infection combined with H. pylori infection is more common.

M A Kazakova

Author for correspondence.
Kazan State Medical University Kazan, Russia

O K Pozdeev

Kazan State Medical Academy Kazan, Russia

Yu V Valeeva

Kazan State Medical University Kazan, Russia

L G Morozova

Kazan State Medical Academy Kazan, Russia

R A Fayzullina

Kazan State Medical University Kazan, Russia

  • Sung J.Y., Costerton J.W. Defense system in the biliary tract against bacterial infection. Digestive Dis. Sci. 1992; 37 (5): 689-696. doi: 10.1007/BF01296423.
  • Malfertheiner P., Megraud F., O’Morain C.A. et al. Management of Helicobacter pylori infection the Maastricht IV / Florence Consensus Report. Gut. 2012; 61 (5): 646-664. doi: 10.1136/gutjnl-2012-302084.
  • Muhammad J.S., Sugiyama T., Zaidi S.F. Gastric pathophysiological and outs of Helicobacter pylori: a review. J. Pak. Med. Assoc. 2013; 63 (12): 1528-1533. PMID: 24397100.
  • Suzuki H., Marshall B.J., Hibi T. Overview: Helicobacter pylori and extragastric disease. Int. J. Hematol. 2006; 84 (4): 291-300. doi: 10.1532/IJH97.06180.
  • Franceschi F., Zuccalа G., Roccarina D., Gasbarrini A. Clinical effects of Helicobacter pylori outside the stomach. Nat. Rev. Gastroenterol. Hepatol. 2014; 11 (4): 234-242. doi: 10.1038/nrgastro.2013.243.
  • Fukuda K., Kuroki T., Tajima Y. et al. Comparative analysis of Helicobacter DNAs and biliary pathology in patients with and without hepatobiliary cancer. Carcinogenesis. 2002; 23 (11): 1927-1931. doi: 10.1093/carcin/23.11.1927.
  • Leong R.W., Sung J.J. Review article: Helicobacter species and hepatobiliary diseases. Aliment. Pharmacol. Ther. 2002; 16 (6): 1037-1045. doi: 10.1046/j.1365-2036.2002.01282.x.
  • Waluga M., Kukla M., Їorniak M. et al. From the stomach to other organs: Helicobacter pylori and the liver. World J. Hepatol. 2015; 28; 7 (18): 2136-2146. doi: 10.4254/wjh.v7.i18.2136.
  • Lee J.W., Lee D.H., Lee J.I. et al. Identification of Helicobacter pylori in gallstone, bile, and other hepatolobiliary tissues of patient with cholecystitis. Gut Liver. 2010; 4 (1): 60-67. doi: 10.5009/gnl.2010.4.1.60.
  • Jahani Sherafat S., Tajeddin E., Reza Seyyed Majidi M. et al. Lack of association between Helicobacter pylori infection and biliary tract diseases. Pol. J. Microbiol. 2012; 61 (4): 319-322.
  • Kandelaki S., Kordzaia D. Helicobacter and hepatobiliary diseases: conceptual view and review of the literature. Georgian Med. News. 2014; (232-233): 92-98. PMID: 25214281.
  • Boonyanugomol W., Chomvarin C., Sripa B. et al. Molecular analysis of Helicobacter pylori virulent - associated genes in hepatobiliary patients. HPB (Oxford). 2012; 14 (11): 754-763. doi: 10.1111/j.1477-2574.2012.00533.x.
  • Дорофейчук В.Г., Потехин П.П., Закомерный А.Г. Неинвазивный способ диагностики пилорического хелиобактериоза. Клин. лаб. диагностика. 1997; (8): 42-44.
  • Рекомендации по диагностике и лечению инфекции Helicobacter pylori у взрослых при язвенной болезни желудка и двенадцатиперстной кишки. Рос. ж. гастроэнтерол., гепатол., колопроктол. 1998; (1): 105-107.


Abstract - 79

PDF (Russian) - 85

© 2017 Kazakova M.A., Pozdeev O.K., Valeeva Y.V., Morozova L.G., Fayzullina R.A.

Creative Commons License

This work is licensed
under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.