Comparative assesment of efficiency application cold atmospheric plasma and biopolymerous coats evaluation of the effectiveness for the treatment of skin burns of iii degree in experiment

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Abstract


The results of application of low temperature cold atmospheric plasma, as well as develop a experimental wound dressing based on chitosan nanofibers and copolyamide, commercial wound dressing material based on a hydrogel of hyaluronic acid in the treatment of skin burns of III degree (ICD-10) in the experimental study. In carrying out the work during the first phase of the study developed an original method for infliction of thermal burn of the skin of small laboratory animals (rodents). Temperature of dehaired skin and temperature of heated by resistive electrical element metal plate was detected by sensor of digital thermometer. Source for generation of cold atmospheric plasma was made by specialists of Saint Petersburg Polytechnic university of Peter the Great. Biopsy material for histological essay was taken on 3, 7, 12, 15, 21, 28 days after treatment. Paraffinic microscopic sections was performed with hematoxylin and eosin, after this microscopic essay was made. The use of low temperature cold atmospheric plasma allows to decrease the frequency of purulent complications, and also helps to reduce the recovery time of the skin by 20% (p < 0.05), but not sufficient to achieve the result that has been proclaimed after an early echarectomy and replacement of surgical defects by wound dressing materials based on natural polymers. It is shown that the wound dressing based on aliphatic copolyamide and chitosan and hydrogel of hyaluronic acid can help significantly accelerate the process of reparative regeneration and histogenesis in the heat-affected zone after echarectomy up to 14.6-46% (p < 0.05).


Evgeny V Zinovyev

Author for correspondence.
evz@list.ru
St Petersburg State Pediatric Medical University, Ministry of Healthcare of the Russian Federation
Russian Federation, Saint Petersburg, Russia

MD, PhD, Dr Med Sci Professor, Department of Hospital Surgery with Traumatology and Military Surgery Courses

Marat S Asadulaev

marat.asadulaev@yandex.ru
St Petersburg State Pediatric Medical University, Ministry of Healthcare of the Russian Federation
Russian Federation, Saint Petersburg, Russia

Department of Surgical Diseases of Childhood

Igor A Komissarov

komissarov_i_a@mail.ru
St Petersburg State Pediatric Medical University, Ministry of Healthcare of the Russian Federation
Russian Federation, Saint Petersburg, Russia

MD, PhD, Dr Med Sci Professor, Head, Department of Surgical Diseases of Childhood

Mihail V Shemet

mvshemet@gmail.com
Peter the Great St Petersburg Polytechnic University
Russian Federation, Saint Petersburg, Russia

PhD, Associate Professor. Department of High Voltage Engineering, Electrical Insulation and Cable Technology

Vladimir E Yudin

yudinve@gmail.com
Peter the Great St Petersburg Polytechnic University
Russian Federation, Saint Petersburg, Russia

Dr Sci Professor, Head. Institute of Physics, Nanotechnology and Telecommunications, Department of Medical Physics, Laboratory of Polymeric Materials for Tissue Engineering and Transplantology

Anton S Shabunin

anton-shab@yandex.ru
Peter the Great St Petersburg Polytechnic University
Russian Federation, Saint Petersburg, Russia

Institute of Physics, Nanotechnology and Telecommunications, Department of Medical Physics, Laboratory of Polymeric Materials for Tissue Engineering and Transplantology

Natalia V Smirnova

nvsmirnoff@yandex.ru
Peter the Great St Petersburg Polytechnic University
Russian Federation, Saint Petersburg, Russia

PhD Researcher. Institute of Physics, Nanotechnology and Telecommunications, Department of Medical Physics, Laboratory of Polymeric Materials for Tissue Engineering and Transplantology

Artem E Kryukov

kryukov14@gmail.com
Peter the Great St Petersburg Polytechnic University
Russian Federation, Saint Petersburg, Russia

Institute of Physics, Nanotechnology and Telecommunications, Department of Medical Physics, Laboratory of Polymeric Materials for Tissue Engineering and Transplantology

Sergey A Lukyanov

stalker125lu@yandex.ru
St Petersburg State Pediatric Medical University, Ministry of Healthcare of the Russian Federation
Russian Federation, Saint Petersburg, Russia

Department of Surgical Diseases of Childhood

Roman G Stoyanovskiy

explabgpmu@gmail.com
St Petersburg State Pediatric Medical University, Ministry of Healthcare of the Russian Federation
Russian Federation, Saint Petersburg, Russia

Assistant Professor, Department of Human Anatomy

Tatiana A Shalonia

tanyaiudina@mail.ru
St Petersburg State Pediatric Medical University, Ministry of Healthcare of the Russian Federation
Russian Federation, Saint Petersburg, Russia

MD, PhD, Assistant Professor, Department of Pathological Anatomy at the Rate of Forensic Medicine

Ilya V Artsimovich

enerdgezer@yandex.ru
St Petersburg State Pediatric Medical University, Ministry of Healthcare of the Russian Federation
Russian Federation, Saint Petersburg, Russia

Department of Surgical Diseases of Childhood

Denis V Kostyakov

kostyakovdv@gmail.com
St Petersburg State Pediatric Medical University, Ministry of Healthcare of the Russian Federation
Russian Federation, Saint Petersburg, Russia

Department of Pathologic Physiology Courses Immunopathology and Medical Informatics

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Copyright (c) 2017 Zinovyev E.V., Asadulaev M.S., Komissarov I.A., Shemet M.V., Yudin V.E., Shabunin A.S., Smirnova N.V., Kryukov A.E., Lukyanov S.A., Stoyanovskiy R.G., Shalonia T.A., Artsimovich I.V., Kostyakov D.V.

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