Comparative analysis of DQB1 и DQA1 class II genes of human leukocyte antigen system in the population of Azerbaijan
- Authors: Akhmedov GA1
-
Affiliations:
- Azerbaijan Medical University
- Issue: Vol 98, No 5 (2017)
- Pages: 704-708
- Section: Theoretical and clinical medicine
- URL: https://kazanmedjournal.ru/kazanmedj/article/view/7020
- DOI: https://doi.org/10.17750/KMJ2017-704
- ID: 7020
Cite item
Full Text
Abstract
Aim. To perform comparative analysis of DQB1 and DQA1 class II genes of the human leukocyte antigen (HLA) system in the population of Azerbaijan.
Methods. We studied the alleles of HLA DQ gene and subtypes of HLA DRB1*04 in 160 children with diabetes mellitus and in 271 healthy children from the population of Azerbaijan. Out of 160 patients, 50.6% (n=81) were boys, 49.4% (n=79) were girls. All patients with diabetes were under the age of 18 years. As a control group, 271 students of the Medical College No. 1 were involved: 79 (29.1%) boys, 192 (70.9%) girls. The collected blood samples were sent for further investigation to the medical genetic laboratory where polymerase chain reaction-based genotyping of the samples was performed.
Results. For the first time, the relationship of diabetes with class II genes of the HLA system was studied. The Azerbaijani were found to have higher risk associated with HLA DQ2 molecule. The molecule HLA DQ8 also increases risk, although it is lower in comparison with HLA DQ2 molecule. What is unusual is that for this population the allele HLA DQB1*0304 was also associated with the risk of diabetes.
Conclusion. The haplotype DQB1*0302-DQA1*03/DQB1*02-DQA1*05 (DQ8/DQ2.5) associated with high risk of diabetes is found in 3% of the representatives of the population of Azerbaijan, which is estimated as the average prevalence of diabetes.
About the authors
G A Akhmedov
Azerbaijan Medical University
Author for correspondence.
Email: gunduzahmadov@gmail.com
Baku, Azerbaijan
References
- Eisenbarth G.S. Immunology of type 1 diabetes. Second Edition. 2004; 170-197.
- Witas H.W., Jedrychowska-Daсska K., Zawicki P. Changes in frequency of IDDM-associated HLA DQB, CTLA4 and INS alleles. Int. J. Immunogenet. 2010; 37 (3): 155-158. doi: 10.1111/j.1744-313X.2010.00896.x.
- Mychaleckyj J.C., Noble J.A., Moonsamy P.V. et al. T1DGC. HLA genotyping in the international type 1 Diabetes Genetics Consortium. Clin. Trials. 2010; 7 (Suppl. 1): 75-87. doi: 10.1177/1740774510373494.
- Dedov I.I., Shestakova M.V., Kuraeva T.L. et al. Nozological heterogeneity, molecular genetics and immunology of autoimmune diabetes mellitus. Vestn. Ross. Akad. Med. Nauk. 2015; (2): 132-138. doi: 10.15690/vramn.v70i2.1305.
- Sayad A., Akbari M.T., Pajouhi M. et al. Investigation the role of gender on the HLA-DRB1 and -DQB1 association with type 1 diabetes mellitus in Iranian patients. Cell J. 2013; 15 (2): 108-115. Epub. 2013 Jul. 2. PMID: 23862111.
- Hamzeh A.R., Nair P., Al Ali M.T. The profile of HLA-DRB1 alleles in Arabs with type 1 diabetes; meta-analyses. HLA. 2016; 87 (1): 25-30. doi: 10.1111/tan.12717.
- Sugihara S., Ogata T., Kawamura T. et all. Japanese Study Group of Insulin Therapy for Childhood and Adolescent Diabetes (JSGIT). HLA-class II and class I genotypes among Japanese children with type 1A diabetes and their families. Pediatr. Diabetes. 2012; 13 (1): 33-44. doi: 10.1111/j.1399-5448.2011.00833.x.
- Zhang X.M., Wang H.Y., Luo Y.Y., Ji L.N. HLA-DQ, DR allele polymorphism of type 1 diabetes in the Chinese population: a meta-analysis. Chin. Med. J. (Engl.) 2009; 122 (8): 980-986. PMID: 19493426.
Supplementary files
