Prevalence of Helicobacter pylori genotypes in patients with gastroduodenal pathology in Kazan

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Abstract

Aim. Investigation of the prevalence of various H. pylori genotypes among children and adult population of Kazan with chronic gastroduodenal pathology.

Methods. The study included 107 patients (49 children and 58 adults) with chronic gastritis/gastroduodenitis and gastric and duodenal ulcer who had H. pylori infection confirmed by molecular genetic method. All patients underwent biospy from antral mucosa during endoscopy for H. pylori verification by polymerase chain reaction and genotyping for сagA and babA genes and iceA and vacA allels.

Results. CagA gene was found in 19 (32.8%) out of 58 adults and 13 (26.5%) out of 49 children. VacA gene was detected in all patients (100%). VacAs2 genotype in children was nearly 1.6 times as frequent as the vacAs1 genotype (61.2 and 36.7% respectively). In adult patients vacAs2 genotype was detected 2.5 times less frequently than vacAs1 (27.6 and 70.7%, respectively). VacAm2 genotype was revealed in 71.4% (35/49) of children and 77.6% (45/58) of adults. IceA2 genotype was identified in 46.9% (23/49) of children and 44.8% (26/58) of adult patients, iceA1 gene - in 20.4% of children and 55.2% of adult patients.

Conclusion. The strains with vacAs2m2 genotype are prevailing in children (42.9%) and determine low toxigenicity of H. pylori strains; vacAs1m2 genotype is predominant among adult patients (53.4%); high prevalence of cagA-negative strains of H. pylori was found both in children and adults (73.5 and 67.2%, respectively).

About the authors

A R Akhtereeva

Kazan State Medical University

Author for correspondence.
Email: sayarabdul@yandex.ru
Kazan, Russia

Yu N Davidyuk

Kazan (Volga Region) Federal University, Institute of Fundamental Medicine and Biology

Email: sayarabdul@yandex.ru
Kazan, Russia

R A Faizullina

Kazan State Medical University

Email: sayarabdul@yandex.ru
Kazan, Russia

K A Ivanovskaya

Republican Clinical Infectious Diseases Hospital named after A.F. Agafonov

Email: sayarabdul@yandex.ru
Kazan, Russia

A G Safin

Kazan (Volga Region) Federal University, Institute of Fundamental Medicine and Biology

Email: sayarabdul@yandex.ru
Kazan, Russia

D D Safina

Kazan (Volga Region) Federal University, Institute of Fundamental Medicine and Biology

Email: sayarabdul@yandex.ru
Kazan, Russia

S R Abdulkhakov

Kazan State Medical University; Kazan (Volga Region) Federal University, Institute of Fundamental Medicine and Biology

Email: sayarabdul@yandex.ru
Kazan, Russia; Kazan, Russia

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© 2017 Akhtereeva A.R., Davidyuk Y.N., Faizullina R.A., Ivanovskaya K.A., Safin A.G., Safina D.D., Abdulkhakov S.R.

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