Advances in Chemical PhysicsAdvances in Chemical PhysicsФизиология растений0015-33033034-6126The Russian Academy of Sciences64815210.31857/S0015330322600334ALLDSGЭКСПЕРИМЕНТАЛЬНЫЕ СТАТЬИResearch ArticleTrans-Factor PTF1 Participates in the Response to Salinity but Does Not Regulate Expression of the psbD Gene in Arabidopsis thalianaТранс-фактор PTF1 участвует в ответе на засоление, но не регулирует экспрессию гена psbD у Arabidopsis thalianaAndreevaA. A.АндрееваА. А.
Russian Federation
nvkudryakova@mail.ruBychkovI. A.БычковИ. А.
Russian Federation
nvkudryakova@mail.ruKudryakovaN. V.КудряковаН. В.
Russian Federation
nvkudryakova@mail.ruKuznetsovV. V.КузнецовВ. В.
Russian Federation
nvkudryakova@mail.ruTimiryazev Institute of Plant Physiology, Russian Academy of SciencesФедеральное государственное бюджетное учреждение науки Институт физиологии растений им. К.А. Тимирязева Российской академии наук01012023701809028012025Copyright ©; 2023, А.А. Андреева, И.А. Бычков, Н.В. Кудрякова, В.В. КузнецовCopyright ©; 2023, А.А. Андреева, И.А. Бычков, Н.В. Кудрякова, В.В. Кузнецов2023А.А. Андреева, И.А. Бычков, Н.В. Кудрякова, В.В. КузнецовА.А. Андреева, И.А. Бычков, Н.В. Кудрякова, В.В. Кузнецовhttps://journals.eco-vector.com/0015-3303/article/view/648152

The existing data on the role of PTF1/TCP13 belonging to the TCP family of transcription factors in regulating expression of a psbD plastid gene encoding a D2 protein of PSII are controversial. To analyze biological functions of PTF1/TCP13, transformed plants expressing PTF1/TCP13 under a β-estradiolinducible promoter were used. PTF1/TCP13 overexpression did not provide the expected increase in the accumulation of psbD transcripts transcribed from BLRP (Blue Light Responsive Promoter), though their level significantly increased under exposure to light or abscisic acid (ABA). PTF1/TCP13 was up-regulated by ABA; moreover, genes of the canonic pathway of the ABA signal transduction were involved in the regulation of PTF1/TCP13 expression. In addition, PTF1/TCP13 was induced in response to salt stress However, in the overexpressing line, salt tolerance and expression of salt stress markers, as well as a number of genes for the synthesis and signaling of ABA, were reduced compared to plants with the normal level of expression of this transcription factor, that is, PTF1/TCP13 acted as a negative regulator of salt stress Thus, PTF1 does not belong to plastid transcription factors. Nevertheless, it represents one of the components of the ABA-dependent regulatory chain capable of modifying expression of nuclear and chloroplast genes in response to changes in homeostasis.

В литературе представлены противоречивые данные относительно роли PTF1/ ТСР13 из семейства транскрипционных факторов TCP в регуляции экспрессии пластидного гена psbD, кодирующего D2 белок ФС II. Для анализа биологических функций PTF1/ ТСР13 нами был использован трансформант, у которого ген PTF1/TCP13 экспрессировался под действием β-эстрадиол-индуцибельного промотора. Оверэкспрессия PTF1/TCP13 не способствовала ожидаемому увеличению накопления транскриптов гена psbD, транскрибируемых с промотора BLRP (Blue Light Responsive Promoter), хотя их уровень существенно повышался под действием света или АБК. PTF1/TCP13 активировался АБК, причем в регуляции экспрессии PTF1/TCP13 принимали участие гены канонического пути передачи сигнала АБК. Кроме того, PTF1/TCP13 индуцировался в ответ на солевой стресс. Однако у оверэкспрессирующей линии были снижены солеустойчивость и экспрессия маркеров солевого стресса, а также ряда генов синтеза и сигналинга АБК по сравнению с аналогами с нормальным уровнем экспрессии этого транс-фактора, то есть, PTF1/TCP13 действовал как отрицательный регулятор солевого стресса. Таким образом, PTF1 не является транскрипционным фактором пластид. Тем не менее, он представляет один из компонентов АБК-зависимой регуляторной цепи, способной модифицировать экспрессию ядерных и хлоропластных генов в ответ на изменение гомеостаза.

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