Advances in Chemical PhysicsAdvances in Chemical PhysicsФизиология растений0015-33033034-6126The Russian Academy of Sciences64815310.31857/S0015330322600802PZCOVAЭКСПЕРИМЕНТАЛЬНЫЕ СТАТЬИUnknownFEATURES OF GROWTH AND INULIN CONTENT IN CALLUS CULTURES Cichorium intybus L. in vitroОсобенности роста и содержания инулина в каллусных культурах Cichorium intybus L. in vitroKirakosyanaE. A.КалашниковаЕ. А.
Russian Federation
kalash0407@mail.ruKirakosyanaR. N.КиракосянР. Н.
Russian Federation
kalash0407@mail.ruTrukhachevaV. I.ТрухачевВ. И.
Russian Federation
kalash0407@mail.ruPankovaaM. G.ПанковаМ. Г.
Russian Federation
kalash0407@mail.ruSuminaA. V.СуминА. В.
Russian Federation
kalash0407@mail.ruRussian State Agrarian University - Moscow Timiryazev Agricultural AcademyФедеральное государственное бюджетное образовательное учреждение высшего образования “Российский государственный аграрный университет − МСХА имени К.А. Тимиряева”0107202370439240128012025Copyright ©; 2023, Kirakosyana E.A., Kirakosyana R.N., Trukhacheva V.I., Pankovaa M.G., Sumina A.V.Copyright ©; 2023, Е.А. Калашникова, Р.Н. Киракосян, В.И. Трухачев, М.Г. Панкова, А.В. Сумин2023Kirakosyana E.A., Kirakosyana R.N., Trukhacheva V.I., Pankovaa M.G., Sumina A.V.Е.А. Калашникова, Р.Н. Киракосян, В.И. Трухачев, М.Г. Панкова, А.В. Суминhttps://journals.eco-vector.com/0015-3303/article/view/648153

In vitro callus cultures of common chicory (Cichorium intybus L.) were obtained and their growth and biochemical characteristics depending on the hormonal composition of the MS medium and the spectral composition of light were studied. The study of the effect of light culture on callus tissue formation and inulin accumulation in it was carried out in opaque grow tents with radiation aligned with the flux density of pho- tosynthetic photons and different ratios of its levels in the region of 660 nm (R, red) and 730 nm (FR, far red). The control variant was placed under white linear fluorescent lamps. The resulting cultures were character- ized by high proliferative activity and the capability for morphogenesis. It has been established that the inter- action of two factors—the presence of auxins in the nutrient medium (IAA or NAA at a concentration of 7.5 mg/L in combination with BAP 0.5 mg/L) and cultivation under light culture conditions (FR > R, FR = R, FR < R)—had a significant impact on the biosynthetic potential of cell cultures. In the obtained cultures, a study of the quantitative content of inulin was carried out. It has been shown that the high content of inulin (7.55–7.95%) in callus cultures was on the MS medium in combination with IAA at FR > R illumination. This is probably due to the fact that well proliferating and highly morphogenic callus tissue was formed under these conditions. The obtained results confirm the hypothesis about the specificity of cultured cells to in vitro synthesize and accumulate secondary metabolites in dedifferentiated cells and the dependence of this process on factors of chemical and physical nature.

Получены каллусные культуры in vitro цикория обыкновенного (Cichorium intybus L.) и исследованы их ростовые и биохимические особенности в зависимости от гормонального состава МС-среды и спектрального состава света. Изучение влияния светокультуры на формирование каллусной ткани и накопление в ней инулина проводили в светонепроницаемых гроутентах с излучением выровненным по плотности потока фотосинтетических фотонов и различным соотношением его уровней в области 660 нм (R – красный) и 730 нм (FR – дальний красный). Контрольный вариант размещали под белыми линейно-люминесцентными лампами. Полученные культуры характеризовались высокой пролиферативной активностью и способностью к морфогенезу. Установлено, что взаимодействие двух факторов – присутствие в составе питательной среды ауксинов (ИУК или НУК в концентрации 7.5 мг/л в сочетании с БАП 0.5 мг/л) и культивирование в условиях светокультуры (FR > R, FR = R, FR < R) – оказало существенное влияние на биосинтетический потенциал культур клеток. В полученных культурах проведено исследование количественного содержания инулина. Показано, что высокое содержание инулина (7.55–7.95%) в каллусных культурах было характерно при выращивании на МС-среде в сочетании с ИУК при режиме освещения FR > R. Вероятно, это обусловлено тем, что именно в этих условиях формировалась хорошо пролиферирующая и высокоморфогенная каллусная ткань. Полученные результаты подтверждают выдвинутую гипотезу о специфичности культивируемых клеток in vitro синтезировать и накапливать вторичные метаболиты в дедиффенцированных клетках и зависимости этого процесса от факторов химической и физической природы.

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