Advances in Chemical PhysicsAdvances in Chemical PhysicsФизиология растений0015-33033034-6126The Russian Academy of Sciences64815410.31857/S0015330322600462GKPQWGЭКСПЕРИМЕНТАЛЬНЫЕ СТАТЬИResearch ArticleBiochemical composition of Euglena gracilis cells during mixotrophic growth in the presence of various organic substratesБиохимический состав клеток Euglena gracilis при миксотрофном росте в присутствии различных органических субстратовGulkE. I.ГулкЕ. И.
Russian Federation
elena.tarakhovskaya@gmail.comZamyatkinaE. B.ЗамяткинаЕ. Б.
Russian Federation
elena.tarakhovskaya@gmail.comBirkemeyerC.БиркемайерК.
Germany
elena.tarakhovskaya@gmail.comTarakhovskayaE. R.ТараховскаяЕ. Р.
Russian Federation
elena.tarakhovskaya@gmail.comSt. Petersburg State UniversityСанкт-Петербургский государственный университетLeipzig UniversityУниверситет ЛейпцигаSt. Petersburg Branch of Vavilov Institute of General Genetics, Russian Academy of SciencesСанкт-Петербургский филиал Института общей генетики им. Н.И. Вавилова Российской академии наук0103202370221422428012025Copyright ©; 2023, Е.И. Гулк, Е.Б. Замяткина, К. Биркемайер, Е.Р. ТараховскаяCopyright ©; 2023, Е.И. Гулк, Е.Б. Замяткина, К. Биркемайер, Е.Р. Тараховская2023Е.И. Гулк, Е.Б. Замяткина, К. Биркемайер, Е.Р. ТараховскаяЕ.И. Гулк, Е.Б. Замяткина, К. Биркемайер, Е.Р. Тараховскаяhttps://journals.eco-vector.com/0015-3303/article/view/648154

The general biochemical composition and profile of low molecular weight metabolites of euglena (Euglena gracilis Klebs.) cells grown in a mixotrophic culture supplemented with 0.5% ethanol, glucose, butanol, glycine, or glycerol was studied. Ethanol and glucose significantly stimulated the growth of the Euglena culture and the accumulation of storage compounds (paramylon and wax esters) in the cells. Butanol also contributed to the division of euglena cells and increased biosynthesis of protein, mono- and disaccharides, carotenoids, and tocopherols. Glycine and glycerol were metabolized more slowly than other substrates, and they accumulated in E. gracilis cells along with their direct derivatives. Glycine did not stimulate the growth of the culture, but it caused the accumulation of paramylon, organic acids of the Krebs cycle, and nitrogen-containing metabolites (chlorophyll, free amino acids, and nitrogenous bases) in the cells. Apparently, glycine is equally effectively used by Euglena as an additional source of both carbon and nitrogen under the conditions of mixotrophy. Assimilation of glycerol was accompanied by the accumulation of wax esters and the amino acids proline and ornithine in Euglena cells. In general, the results obtained demonstrate the ability of Euglena to significantly rearrange its metabolism during the assimilation of organic substrates of various chemical nature. Our data can be used in the context of E. gracilis practical applications.

Проведено исследование общего биохимического состава и профиля низкомолекулярных метаболитов клеток эвглены (Euglena gracilis Klebs.), растущих в миксотрофной культуре с добавлением 0.5% этанола, глюкозы, бутанола, глицина или глицерина. Этанол и глюкоза существенно стимулировали рост культуры эвглены и накопление в клетках запасных соединений (парамилона и восковых эфиров). Бутанол также способствовал делению клеток эвглены и усилению биосинтеза белка, моно- и дисахаридов, каротиноидов и токоферолов. Глицин и глицерин усваивались медленнее, чем другие субстраты, и накапливались в клетках E. gracilis вместе со своими непосредственными производными. Глицин не стимулировал рост культуры, но вызывал накопление в клетках парамилона, органических кислот цикла Кребса и азотсодержащих метаболитов (хлорофилла, свободных аминокислот и азотистых оснований). По-видимому, в условиях миксотрофии глицин одинаково эффективно используется эвгленой как дополнительный источник и углерода, и азота. Усвоение глицерина сопровождалось накоплением в клетках эвглены восковых эфиров, а также аминокислот пролина и орнитина. В целом, полученные результаты демонстрируют способность эвглены существенно перестраивать свой метаболизм при усвоении органических субстратов различной химической природы. Данные могут быть использованы в контексте прикладного применения E. gracilis.

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