Hygiene and SanitationHygiene and SanitationГигиена и санитария0016-99002412-0650Federal Scientific Center of Hygiene named after F.F. Erisman63840110.47470/0016-9900-2020-99-12-1360-1364ENVIRONMENTAL HYGIENEГИГИЕНА ОКРУЖАЮЩЕЙ СРЕДЫComparison of the pathogenic potential of Klebsiella pneumoniae isolates from human intestinal microbiota, surface waters, and sewageСравнение патогенного потенциала изолятов Klebsiella pneumonia, выделенных из кишечной микробиоты человека, из поверхностных и сточных водhttps://orcid.org/0000-0001-7086-0899PayGalina V.ПайГ. В.
Russian Federation
https://orcid.org/0000-0003-3554-7690RakitinaDaria V.РакитинаД. В.
Russian Federation
https://orcid.org/0000-0002-9133-3665PankovaMarina N.ПаньковаМ. Н.
Russian Federation
https://orcid.org/0000-0002-7942-8004YudinSergey M.ЮдинС. М.
Russian Federation
https://orcid.org/0000-0003-4772-9686ZagaynovaAngelika V.ЗагайноваАнжелика Владимировна
Russian Federation

MD, Ph.D., Head of Microbiology and parasitology laboratory in the Centre for Strategic Planning and Management of Biomedical Health Risks of the Federal Medical Biological Agency, Moscow, 119121, Russian Federation.

e-mail: mgoshin@cspmz.ru 

Канд. биол. наук, зав. лаб. микробиологии и паразитологии ФГБУ «ЦСП» ФМБА России, 119121, Москва.

e-mail: angelikaangel@mail.ru

angelikaangel@mail.ruCentre for Strategic Planning and Management of Biomedical Health Risks of the Federal Medical Biological AgencyФГБУ «Центр стратегического планирования и управления медико-биологическими рисками здоровью» Федерального медико-биологического агентства1512202099121360136425102024Copyright ©; 2020, Pay G.V., Rakitina D.V., Pankova M.N., Yudin S.M., Zagaynova A.V.Copyright ©; 2020, Пай Г.В., Ракитина Д.В., Панькова М.Н., Юдин С.М., Загайнова А.В.2020Pay G.V., Rakitina D.V., Pankova M.N., Yudin S.M., Zagaynova A.V.Пай Г.В., Ракитина Д.В., Панькова М.Н., Юдин С.М., Загайнова А.В.https://journals.eco-vector.com/0016-9900/article/view/638401

Introduction. Klebsiella pneumoniae, an opportunistic pathogen responsible for nosocomial and community-acquired infections, is present in soil and water. Still, the virulent potential of K. pneumoniae isolates from the environment is mostly unknown. Since isolates with multiple antibiotic resistance are currently considered to be the main threat of environmental pollution, the bulk of ecological studies of K. pneumoniae are devoted to the identification of just such isolates (mainly producers of beta-lactamases).

Material and methods. In this study, 42 isolates of K. pneumoniae isolated from wastewater, 19 from surface water sources, and 63 isolates from the intestinal microbiota of conventionally healthy cases were analyzed by PCR for the presence of potential virulence genes (ybts, kfu, rmpA, mrkD, K2 , alls, magA, iutA).

Results. As a result of the analysis, a statistically significant heterogeneity of the studied samples was revealed. Isolates from wastewater showed the highest proportion and spectrum of virulent genes (8 out of 8). Isolates from surface sources were statistically indistinguishable from isolates isolated from the intestinal microbiota of “conventionally healthy” people, while pathogenic determinants were also detected in these groups.

Conclusion. K. pneumoniae isolates from surface sources did not statistically differ from isolates from feces of “practically healthy” ones, although some of the virulent genes were also detected in these isolates. Wastewater can serve as a reservoir for highly virulent K. pneumoniae, to a greater extent than the intestines of healthy people, and can serve as a risk to public health. Additional studies with larger samples and a more comprehensive range of virulent traits are needed to predict the spread of hypervirulent strains.

Введение. Klebsiella pneumoniae является условно патогенным возбудителем, который отвечает за возникновение внутрибольничных и внебольничных инфекций, присутствует в почвенной и водной среде, но вирулентный потенциал изолятов K. pneumoniae из окружающей среды в значительной степени неизвестен. Поскольку главной угрозой загрязнения внешней среды в настоящий момент считаются изоляты с множественной устойчивостью к антибиотикам, то основная масса исследований K. pneumoniae из окружающей среды посвящена выявлению именно таких изолятов (в основном продуцентов бета-лактамаз).

Материал и методы. В работе 42 изолята K. рneumoniae, выделенных из сточных вод, 19 – из поверхностных водных источников и 63 изолята из кишечной микробиоты «практически здоровых» людей проанализированы методом ПЦР на наличие потенциальных генов вирулентности (ybts, kfu, rmpA, mrkD, K2, alls, magA, iutA).

Результаты. В результате анализа выявлена статистически значимая неоднородность исследуемых выборок. Изоляты, выделенные из сточных вод, показали наибольшую долю и спектр вирулентных генов (8 из 8). Изоляты из поверхностных источников статистически оказались неразличимы с изолятами, выделенными из кишечной микробиоты «практически здоровых» людей, при этом и в этих группах выявлялись патогенные детерминанты.

Заключение. Изоляты К. pneumoniae из поверхностных источников статистически не различались с изолятами из кала «практически здоровых», хотя часть вирулентных генов выявлена и в этих изолятах. Сточные воды могут служить резервуаром для высоковирулентных представителей К. pneumoniae, в большей степени, чем кишечник здоровых людей, могут служить риском для здоровья населения. Необходимы дополнительные исследования с более объёмными выборками и широким спектром вирулентных признаков для прогнозирования распространения гипервирулентных штаммов.

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