Mycology and PhytopathologyMycology and PhytopathologyМикология и фитопатология0026-36483034-5421The Russian Academy of Sciences65517310.31857/S0026365623600104RKYILMEXPERIMENTAL ARTICLESЭКСПЕРИМЕНТАЛЬНЫЕ СТАТЬИPaludisphaera mucosa sp. nov., a Novel Planctomycete of the Family Isosphaeraceae from a Boreal FenPALUDISPHAERA MUCOSA SP. NOV., новый планктомицет семейства ISOSPHAERACEAE из бореального низинного болотаIvanovaA. A.ИвановаА. А.ivanovastasja@gmail.comNaumoffD. G.НаумовД. Г.ivanovastasja@gmail.comKulichevskayaI. S.КуличевскаяИ. С.ivanovastasja@gmail.comMeshcheriakovaA. A.МещеряковаА. А.ivanovastasja@gmail.comDedyshS. N.ДедышС. Н.ivanovastasja@gmail.comWinogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of SciencesИнститут микробиологии им. С.Н. Виноградского, Федеральный исследовательский центр “Фундаментальные основы биотехнологии” РАНMoscow State University, Faculty of Soil ScienceМосковский государственный университет им. М.В. Ломоносова, почвенный факультет0107202392434735710022025Copyright ©; 2023, А.А. Иванова, Д.Г. Наумов, И.С. Куличевская, А.А. Мещерякова, С.Н. ДедышCopyright ©; 2023, А.А. Иванова, Д.Г. Наумов, И.С. Куличевская, А.А. Мещерякова, С.Н. Дедыш2023А.А. Иванова, Д.Г. Наумов, И.С. Куличевская, А.А. Мещерякова, С.Н. ДедышА.А. Иванова, Д.Г. Наумов, И.С. Куличевская, А.А. Мещерякова, С.Н. Дедышhttps://journals.eco-vector.com/0026-3648/article/view/655173

Abstract—Planctomycetes are common inhabitants of northern wetland ecosystems. In this study, a new planctomycete of the genus Paludisphaera, strain Pla2T, was isolated from a boreal fen in Russia. The novel isolate was represented by nonmotile, pink-pigmented, spherical cells that multiplied by budding and occurred singly or were assembled in small aggregates. Strain Pla2T was a chemoorganotrophic, psychrotolerant mesophile with a growth optimum at pH 5.5‒6 and 15‒20°C. The preferred growth substrates were polysaccharides, including xylan, xanthan gum, and phytagel, as well as some sugars. The 16S rRNA gene sequence of strain Pla2T displayed the highest similarity (97.9%) to that of ‘Paludisphaera soli’ JC670T isolated from highland soil of the western Himalayas. With other members of the genus Paludisphaera, “P. rhizosphaerae” JC665T and P. borealis PX4T, this similarity was 97.0 and 93.8%, respectively. The genome of strain Pla2T was 8.21 Mb in size and contained about 6500 protein-coding genes and 3 copies of the rRNA operon. The DNA G + C content was 67 mol %. The average nucleotide identity between the genome sequence of strain Pla2T and those of previously described members of the genus Paludisphaera was between 79.4 and 82.6%. This genotypic distance as well as several phenotypic differences allowed classifying the new planctomycete from a fen as representing a novel species of the genus Paludisphaera, Paludisphaera mucosa sp. nov. with the type strain Pla2T (=KCTC92668T = VKM B-3698T).

Планктомицеты являются типичными обитателями северных болотных экосистем. В настоящей работе новый планктомицет рода Paludisphaera, штамм Pla2T, был выделен из низинного болота бореальной зоны России. Изолят был представлен розово-пигментированными, одиночными или собранными в небольшие группы неподвижными сферическими клетками, размножающимися почкованием. Штамм Pla2T являлся хемоорганотрофным психротолерантным мезофилом с оптимумом роста при pH 5.5‒6 и температуре 15‒20°C. Предпочтительными ростовыми субстратами нового планктомицета являлись полисахариды ксилан, ксантановая камедь и фитагель, а также некоторые сахара. Наиболее высокое сходство последовательности гена 16S рРНК штамма Pla2T (97.9%) наблюдалось с последовательностью планктомицета “Paludisphaera soli” JC670Т, выделенного из почвы высокогорья Гималаев. С другими представителями рода Paludisphaera, “P. rhizosphae-rae” JC665Т и P. borealis PX4Т, это сходство составляло 97.0 и 93.8% соответственно. Геном штамма Pla2T, размером 8.21 млн п.о., содержал около 6.5 тыс. белок-кодирующих генов и 3 копии оперона рРНК. Содержание пар Г + Ц в ДНК составляло 67 мол. %. Сходство нуклеотидных последовательностей генома штамма Pla2Т и ранее описанных представителей рода Paludisphaera составило от 79.4 до 82.6%. Эти генотипические отличия, а также ряд фенотипических особенностей позволили отнести планктомицет из низинного болота к новому виду рода Paludisphaera, Paludisphaera mucosa sp. nov. с типовым штаммом Pla2T (=KCTC92668T = VKM B-3698T).

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