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Persistent profile of Escherichia coli and Klebsiella pneumoniae strains with genetic determinants of iron chelaters

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1. Title Title of document Persistent profile of Escherichia coli and Klebsiella pneumoniae strains with genetic determinants of iron chelaters
2. Creator Author's name, affiliation, country O. V. Bukharin; Orenburg Federal Research Center, Ural Branch of the Russian Academy of Sciences; Russian Federation
2. Creator Author's name, affiliation, country E. V. Ivanova; Orenburg Federal Research Center, Ural Branch of the Russian Academy of Sciences; Russian Federation
2. Creator Author's name, affiliation, country I. A. Zdvizhkova; Orenburg Federal Research Center, Ural Branch of the Russian Academy of Sciences; Russian Federation
2. Creator Author's name, affiliation, country N. B. Perunova; Orenburg Federal Research Center, Ural Branch of the Russian Academy of Sciences; Russian Federation
3. Subject Discipline(s)
3. Subject Keyword(s) opportunistic enterobacteria; genetic determinants of colibactin and aerobactin; persistent and pathogenic potential
4. Description Abstract

Abstract. The objective of this study is to characterize the relationship between the persistent profile and the ability to produce iron-binding compounds of intestinal isolates of E. coli and K. pneumoniae with the genetic determinants iucBC and clbBN, both at the phenotypic and genetic levels. The use of designed primers and a developed unified multiplex-PCR algorithm permitted the establishment of a wide frequency of occurrence (44.0–80.0%) among opportunistic strains of E. coli and K. pneumoniae cultures with genetic determinants clbBN and iucBC, which encode the synthesis of aerobactin and colibactin. The presence of the clbBN/iucBC genes in the genetic apparatus of enterobacteria was found to result in a pronounced ability to produce iron-binding compounds and a wide range of persistent characteristics (antilysozyme, anticarnosine, antipeptide activity with respect to TNFα, antiimmunoglobulin activity with respect to IgM/IgG and biofilm formation). A comparison of the genomes of sequenced clbBN+iucBC+ strains with those of clbBN-iucBC- strains revealed the presence of genes essential for the biosynthesis and transport of aerobactin, a comprehensive list of pks island genes, and the existence of known homologues of the lysozyme inhibitor determinants Ivy and MliC, EspP (E. coli M-17) and PliC, LprI (K. pneumoniae ICIS-278_PBV and K. pneumoniae ICIS-277_SVA) were identified. Therefore, strains of E. coli and K. pneumoniae that possess the genetic determinants of iron chelators, namely clbBN and iucBC, exhibit both genotypic and phenotypic indications of siderophore production and protease activity against host antimicrobial factors. This allows for the use of these strains as markers of the pathogenic and persistent potential of opportunistic enterobacteria.

5. Publisher Organizing agency, location The Russian Academy of Sciences
6. Contributor Sponsor(s) Ministry of Science and Higher Education of the Russian Federation (075-00879-22-00)
7. Date (DD-MM-YYYY) 10.05.2025
8. Type Status & genre Peer-reviewed Article
8. Type Type Research Article
9. Format File format
10. Identifier Uniform Resource Identifier https://journals.eco-vector.com/0026-3656/article/view/680837
10. Identifier Digital Object Identifier (DOI) 10.31857/S0026365625020042
11. Source Title; vol., no. (year) Microbiology; Vol 94, No 2 (2025)
12. Language English=en ru
13. Relation Supp. Files Figure. Electropherogram of the results of separation of aerobactin (iucBC) (a) and colibactin (clbBN) (b) amplicons in enterobacteria strains. MW – molecular weight scale. (223KB)
14. Coverage Geo-spatial location, chronological period, research sample (gender, age, etc.)
15. Rights Copyright and permissions Copyright (c) 2025 Russian Academy of Sciences