Voenno-medicinskij žurnalVoenno-medicinskij žurnal0026-9050Eco-Vector8265010.52424/00269050_2021_342_8_28Research ArticleHistological and immunohistochemical changes in the skin of the face, developing as a result of the effects of combined laser radiationShaninaN. Ashanina_n@mail.ruYakovenkoA. I-PatrushevA. V-SamtsovA. Vavsamtsov@mail.ruPlakhovV. N-The S.M.Kirov Military Medical Academy, Ministry of Defense of the Russian Federation150820213428283206102021Copyright © 2021, Eco-Vector2021In order to assess pathological changes in the skin after exposure to the combined Er:YAG and Nd:YAG laser, 15 patients (3 men and 12 women) aged 38 to 66 years were examined with signs of aging of the skin of the face and neck (Fitzpatrick phototype I-III). Two procedures of combined (erbium and neodymium) laser irradiation were carried out on the Fotona SP Dynamis apparatus according to the Fotona 4D technique with an interval of 1 month. Skin biopsy was performed on all patients at the screening and 2 months after the start of the study. Histological examination after laser exposure showed an increase in the thickness of the epidermis, an increase in the number of capillaries in the dermis. Immunohistochemical methods revealed increased expression of the CD34 antigen in the zones of newly formed vessels, as well as an increase in the number and density of collagen fibers. Combined laser radiation showed a high efficiency of remodeling of the skin of the face and neck.age-related skin changeslaser therapyerbium and neodymium lasersEr:YAG and Nd:YAGskin biopsyhistological and immunohistochemical changes of the skinвозрастные изменения кожилазерная терапияэрбиевый и неодимовый лазерыEr:YAG и Nd:YAGбиопсия кожигистологические и иммуногистохимические изменения кожи[Пономаренко Г.Н., Спокойный Л.Б., Ключарева С.В. Высокоинтенсивные лазерные технологии в дерматокосметологии. - СПб: Санкт-Петербург XXI век, 2012. - 208 с.][Шанина Н.А., Патрушев А.В., Самцов А.В. и др. Возможности коррекции возрастных изменений кожи лица и шеи с помощью лазерных технологий // Вестн. Рос. воен.-мед. акад. - 2018. - № 1 (61). - С. 29-34.][Bitter P.H. Noninvasive rejuvenation of photodamaged skin using serial, full-face intense pulsed light treatments // Dermatologic Surgery. - 2000. - Vol. 26, N 9. - P. 835-842.][Fisher G.J., Varani J., Voorhees J.J. Looking older: fibroblast collapse and therapeutic implications // Arch. Dermatol. - 2008. - Vol. 144. - P. 666-672.][Lask G., Lee P.K., Seyfzadeh M. et al. Nonablative laser treatment of facial rhytides. In: Anderson R.R., Bartels K.E., Bass L.S. eds. Analysis of Nonablative Skin Resurfacing. - Bellingham, Wash: SPIE, 1997. - P. 338-349.][Marini L. SPF-RR sequential photothermal fractional resurfacing and remodeling with the variable pulse Er:YAG laser and scanner-assisted Nd:YAG laser // Journal of Cosmetic and Laser Therapy. - 2009. - Vol. 11. - P. 202-211.][Matts P.J., Fink B, Grammer K. et al. Color homogeneityand visual perception of age, health, and attractiveness of female facial skin // J. Am. Acad. Dermatol. - 2007. - Vol. 57. - P. 977-984.][Mei-Heng Tan, Jeffrey S. Dover, Te-Shao Hsu et al. Clinical Evaluation of Enhanced Nonablative Skin Rejuvenation Using a Combination of a 532 and a 1,064 nm Laser // Lasers in Surgery and Medicine. - 2004. - Vol. 34. - P. 439-445.][Ross E.V., Sajben F.P., Hsia J. et al. Nonablative skin remodeling: selective dermal heating with mid-infrared laser and contact cooling combination // Lasers Surg. Med. - 2000. - Vol. 26. - P. 186-195.]