Cell and Tissue BiologyCell and Tissue BiologyЦитология0041-37713034-6061The Russian Academy of Sciences66959210.31857/S0041377124030037PENZNVArticlesСтатьиResearch ArticleThe Dual Effect of Lithium Chloride on the Efficiency of Generating Mouse-Induced Pluripotent Stem CellsДвойственное влияние хлорида лития на эффективность образования иПСК мышиKuznetsovA. V.КузнецовА. В.
Russian Federation
atsimokha@incras.ruSkvortsovaE. V.СкворцоваЕ. В.
Russian Federation
atsimokha@incras.ruTomilinA. N.ТомилинА. Н.
Russian Federation
atsimokha@incras.ruTsimokhaA. S.ЦимохаА. С.
Russian Federation
atsimokha@incras.ruInstitute of Cytology RASИнститут цитологии РАН1505202466323424127022025Copyright ©; 2024, Russian Academy of SciencesCopyright ©; 2024, Российская академия наук2024Russian Academy of SciencesРоссийская академия наукhttps://journals.eco-vector.com/0041-3771/article/view/669592

Somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) using certain factors. The low efficiency of the reprogramming, as well as the heterogeneity of iPSCs, limits the potential application for iPSCs in cell therapy. Here, we show that lithium chloride (LiCl), a known activator of the Wnt signaling pathway, reduces or enhances the efficiency of iPSC generation from mouse embryonic fibroblasts (MEFs) depending on the timing of its addition during the reprogramming. Our results not only demonstrate a method to improve the efficiency of iPSC formation by LiCL, but also indicate its dual role in this process.

Соматические клетки могут быть репрограммированы в индуцированные плюрипотентные стволовые клетки (иПСК) с помощью определенных факторов. Низкая эффективность процесса репрограммирования ограничивает потенциал их применения для фундаментальных исследований, а гетерогенность получаемых иПСК – в клеточной терапии. В настоящей работе мы показываем, что хлорид лития (LiCl), известный активатор сигнального пути Wnt, снижает или усиливает эффективность генерации иПСК из эмбриональных фибробластов мыши (МЭФ) в зависимости от момента его добавления в течение процесса репрограммирования. Наши результаты не только демонстрируют способ улучшения эффективности формирования иПСК, но также указывают на двойную роль LiCl в этом процессе.

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