Cell and Tissue BiologyCell and Tissue BiologyЦитология0041-37713034-6061The Russian Academy of Sciences66959610.31857/S0041377124030076PEHBCBArticlesСтатьиResearch ArticleCytoplasmic Granulation of Human Oocytes at the Germinal Vesicle Stage as a Predictor of Their Ability to Spontaneous Maturation in Stimulated Cycles of In Vitro FertilizationГрануляция цитоплазмы ооцитов человека на стадии зародышевого пузырька как предиктор способности к спонтанному созреванию в стимулированных циклах экстракорпорального оплодотворенияSalimovD. F.СалимовД. Ф.
Russian Federation
dfsalimov@mail.ruBogolyubovaI. O.БоголюбоваИ. О.
Russian Federation
dfsalimov@mail.ruBogolyubovD. S.БоголюбовД. С.
Russian Federation
dbogol@mail.ruClinical Institute of Reproductive MedicineКлинический институт репродуктивной медициныInstitute of Cytology of the Russian Academy of SciencesИнститут цитологии РАНSt. Petersburg State Pediatric Medical UniversityСанкт-Петербургский государственный педиатрический медицинский университет1505202466326827327022025Copyright ©; 2024, Russian Academy of SciencesCopyright ©; 2024, Российская академия наук2024Russian Academy of SciencesРоссийская академия наукhttps://journals.eco-vector.com/0041-3771/article/view/669596

Although in vitro fertilization (IVF) programs usually use mature (metaphase II, MII) oocytes, it is also possible to use diplotene oocytes at the germinal vesicle (GV) stage after their in vitro maturation (IVM). Morphological characteristics of native GV oocytes are promising predictors their capacity of spontaneous maturation in stimulated cycles. The purpose of this work was to analyze the patterns of cytoplasmic granulation of GV oocytes that differ in their ability to resume and complete meiotic maturation in vitro. It has been shown that the central granulation pattern negatively correlates with the ability of GV oocytes to spontaneously mature in vitro.

Несмотря на то, что в программах экстракорпорального оплодотворения обычно используют зрелые ооциты, находящиеся на стадии метафазы II мейоза, возможно использование диплотенных ооцитов на стадии зародышевого пузырька (germinal vesicle, GV) после их созревания in vitro (in vitro maturation, IVM). Морфологическая характеристика нативных GV-ооцитов перспективна с точки зрения выявления предикторов для прогнозирования их способности к самопроизвольному созреванию в стимулированных циклах. Целью работы являлся анализ паттернов грануляции цитоплазмы GV-ооцитов, различающихся по способности к возобновлению и завершению мейотического созревания in vitro. Показано, что центральный паттерн грануляции негативно коррелирует со способностью GV-ооцитов к спонтанному созреванию in vitro.

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