Advances in Current BiologyAdvances in Current BiologyУспехи современной биологии0042-13243034-6347The Russian Academy of Sciences65319810.31857/S0042132424030024PSCJEWArticlesСтатьиResearch ArticleStructure and Morphogenetic Properties of Collagen Matrixes Obtained from Connective Tissue Sheaths of Paravertebral TendonsСтруктура и морфогенетические свойства коллагеновых матриц, полученных из соединительнотканных оболочек паравертебральных сухожилийGaidashА. А.ГайдашА. А.
Belarus
algaidashspb@gmail.comKulakA. I.КулакА. И.
Belarus
algaidashspb@gmail.comKrut’koV. K.КрутькоВ. К.
Belarus
suber@igic.bas-net.byBlinovaM. I.БлиноваМ. И.
Russian Federation
algaidashspb@gmail.comMusskayaO. N.МусскаяО. Н.
Belarus
algaidashspb@gmail.comAleksandrovaS. A.АлександроваС. А.
Russian Federation
algaidashspb@gmail.comSkrotskayaK. V.СкроцкаяК. В.
Belarus
algaidashspb@gmail.comKulchitskyV. A.КульчицкийВ. А.
Belarus
algaidashspb@gmail.comInstitute of General and Inorganic Chemistry, National Academy of Sciences of BelarusИнститут общей и неорганической химии Национальной академии наук БеларусиInstitute of Cytology, Russian Academy of SciencesИнститут цитологии Российской академии наукResearch Institute for Physical Chemical Problems, Belarusian State UniversityНаучно-исследовательский институт физико-химических проблем Белорусского государственного университетаInstitute of Physiology, National Academy of Sciences of BelarusИнститут физиологии Национальной академии наук Беларуси18052024144326529002022025Copyright ©; 2024, Russian Academy of SciencesCopyright ©; 2024, Российская академия наук2024Russian Academy of SciencesРоссийская академия наукhttps://journals.eco-vector.com/0042-1324/article/view/653198

The morphogenetic properties of a collagen gel prepared by acetic acid extraction from the tendon sheaths (peritenons) of the paravertebral tendons of Wistar rats were studied. The gel was used as a substrate during in vitro cultivation together with mesenchymal stromal cells for 14 days in the growth and osteogenic incubation media. It has been established that the collagen framework of the peritenon substrate is strengthened by increasing the connectivity of fibrillar nodes and is structured with the formation of lamellar and tangle formations. Sesamoid globules, penetrating into the substrate from the initial peritenon gel, during cultivation remain inert in the growth medium, but exhibit an increased ability to structure calcium phosphates in the osteogenic medium. The formation of cell-mediated structures occurs by directions of fibro-, tendo-, ligament- and osteogenic differentiation. The fibrogenic direction provides a structuring framework; the tenogenic direction – the formation of embryonic tendons according to the mechanism of lateral assembly of collagen subfibrils on cell surfaces and their autonomization in the form of tendon filament primordia; the ligamentogenic direction – structuring of collagen ribbons associated with tangles and elastic fibers; the osteogenic direction – the formation of lamellar, trabecular and nodular osteoid structures through intramembranous ossification, accompanied by activation of alkaline phosphatase and mineralization. The formation of enthesis predictors is the organization of commissures between mechanically different-phase components of osteoid structures and frame. A classification of taxonomic forms has been developed and a hypothesis has been proposed about the role of evolutionary tools in the structuring of the collagen framework in tissue cultures in vitro. The classification of taxonomic forms has been developed and a hypothesis has been proposed about the role of evolutionary tools in the structuring of the collagen framework in tissue cultures in vitro.

Изучены морфогенетические свойства коллагенового геля, полученного ацетатной экстракцией из соединительнотканных оболочек (перитенонов) паравертебральных сухожилий крыс Вистар. Гель использовали в качестве подложки при культивировании in vitro совместно с мезенхимальными стромальными клетками в течение 14 сут в ростовой и остеогенной инкубационных средах. Установлено, что коллагеновый каркас перитеноновой подложки приобретает прочность за счет увеличения связности фибриллярных узлов и структурирован с формированием пластинчатых и клубковых образований. Сесамовидные глобулы, проникающие в подложку из исходного перитенонового геля, инертны в ходе культивирования в ростовой среде, а в остеогенной – проявляют повышенную способность к структурированию кальцийфосфатов. Формирование клеточно-опосредованных структур происходит по направлениям фибро-, тендо-, лигаменто- и остеогенной дифференцировок. Фиброгенное направление обеспечивает коллагеновым материалам структурирующийся каркас; теногенное – формирование сухожилий эмбрионального типа по механизму латеральной сборки коллагеновых субфибрилл на поверхностях клеток и их автономизацию в виде зачатков сухожильных нитей; лигаментогенное – структурирование коллагеновых лент, ассоциированных с клубками и эластическими волокнами; остеогенное – образование ламеллярных, трабекулоподобных и узелковых остеоидных структур путем внутримембранозной оссификации, сопровождающейся активацией щелочной фосфатазы и минерализации. Формирование предикторов энтез – организация комиссур между механически разнофазными компонентами остеоидных структур и каркаса. Разработана классификация таксономических форм и предложена гипотеза о роли эволюционных инструментов в структурировании коллагенового каркаса в тканевых культурах in vitro.

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