Features of the immune and genetic profile in children with musculoskeletal pathology under exposure to biocontamination with aluminum compounds
- Authors: Zaitseva N.V.1, Shirinkina A.S.1, Dolgikh O.V.1, Kazakova O.A.1
-
Affiliations:
- Federal Scientific Center for Medical and Preventive Health Risk Management Technologies
- Issue: Vol 69, No 4 (2025)
- Pages: 361-366
- Section: TOPICAL ISSUES OF HYGIENE
- Submitted: 18.03.2026
- URL: https://journals.eco-vector.com/0044-197X/article/view/704677
- DOI: https://doi.org/10.47470/0044-197X-2025-69-4-361-366
- EDN: https://elibrary.ru/fhxfzi
- ID: 704677
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Abstract
Introduction. The musculoskeletal (MS) system stores heavy metals like aluminum, potentially causing bone defects in polluted environments.
The purpose of the study is to evaluate the immune and genetic profile in children with MS diseases under exposure to aluminum biocontamination.
Materials and methods. One hundred sixty six children from an aluminum province were selected to make up observation with diagnosis as 66, M85.8 «Other specified disorders of bone density and structure») and comparison (100 healthy) groups. The following iondices were estimated: CD19+ (cytofluorometry), IgG to aluminum (allergosorbent testing), osteocalcin (EIA), IgG, IgM, IgA (Mancini). PCR determined TLR4 A8595G (rs1927911) and VDR C>T (rs2228570) polymorphisms.
Results. The aluminum content in blood in examined children with MS diseases was 0.0317 ± 0.0051 mg/dm³, which is 2.66 times higher than in the comparison group. IgG to aluminum in 74.5% of thr children exceeded the reference range. N-osteocalcin was reduced by 1.13 times. CD19+ hyperexpression (2.36 times), IgG increase by 1.2 times and IgA deficiency by 1.32 times were established. In the observation group against the comparison group, a significant increase was established in the frequency both of the variant allele G TLR4 and the variant allele C and the CC genotype VDR, by by 1.9 times and 1.4–2.6 times accoridngly.
Research limitations. The sytudy sample was limited to children aged 3–6 years living in a zone exposed to aluminum production emissions; for the observation group, diagnosis M85.8.
Conclusion. We established activation in the B-lymphocytes→reagins system, CD19+→IgG→specific IgG to aluminum compartments, which may indicate that immunity participoates in the formation of MS pathology. The presence of variant alleles and genotypes of candidate genes VDR C>T (rs2228570), TLR4 A8595G (rs1927911) increases the risk of metabolic and immune disorders associated with bone tissue by 1.2–1.6 times.
The purpose of the study is to evaluate the immune and genetic profile in children with MS diseases under exposure to aluminum biocontamination.
Materials and methods. One hundred sixty six children from an aluminum province were selected to make up observation with diagnosis as 66, M85.8 «Other specified disorders of bone density and structure») and comparison (100 healthy) groups. The following iondices were estimated: CD19+ (cytofluorometry), IgG to aluminum (allergosorbent testing), osteocalcin (EIA), IgG, IgM, IgA (Mancini). PCR determined TLR4 A8595G (rs1927911) and VDR C>T (rs2228570) polymorphisms.
Results. The aluminum content in blood in examined children with MS diseases was 0.0317 ± 0.0051 mg/dm³, which is 2.66 times higher than in the comparison group. IgG to aluminum in 74.5% of thr children exceeded the reference range. N-osteocalcin was reduced by 1.13 times. CD19+ hyperexpression (2.36 times), IgG increase by 1.2 times and IgA deficiency by 1.32 times were established. In the observation group against the comparison group, a significant increase was established in the frequency both of the variant allele G TLR4 and the variant allele C and the CC genotype VDR, by by 1.9 times and 1.4–2.6 times accoridngly.
Research limitations. The sytudy sample was limited to children aged 3–6 years living in a zone exposed to aluminum production emissions; for the observation group, diagnosis M85.8.
Conclusion. We established activation in the B-lymphocytes→reagins system, CD19+→IgG→specific IgG to aluminum compartments, which may indicate that immunity participoates in the formation of MS pathology. The presence of variant alleles and genotypes of candidate genes VDR C>T (rs2228570), TLR4 A8595G (rs1927911) increases the risk of metabolic and immune disorders associated with bone tissue by 1.2–1.6 times.
About the authors
Nina V. Zaitseva
Federal Scientific Center for Medical and Preventive Health Risk Management Technologies
Email: znv@fcrisk.ru
ORCID iD: 0000-0003-2356-1145
DSc (Medicine), Professor, Academician of the Russian Academy of Sciences, Federal Scientific Center for Medical and Preventive Health Risk Management Technologies, Perm, 614045, Russian Federation
Alisa S. Shirinkina
Federal Scientific Center for Medical and Preventive Health Risk Management Technologies
Email: shirinkina.ali@yandex.ru
ORCID iD: 0000-0001-7166-2448
researcher of the Department of Immunobiological Diagnostic Methods, Federal Scientific Center for Medical and Preventive Health Risk Management Technologies, Perm, 614045, Russian Federation
Oleg V. Dolgikh
Federal Scientific Center for Medical and Preventive Health Risk Management Technologies
Email: oleg@fcrisk.ru
ORCID iD: 0000-0003-4860-3145
DSc (Medicine), Professor, Head of the Department of Immunobiological Diagnostic Methods, Federal Scientific Center for Medical and Preventive Health Risk Management Technologies, Perm, 614045, Russian Federation
Olga A. Kazakova
Federal Scientific Center for Medical and Preventive Health Risk Management Technologies
Email: chakina2011@yandex.ru
ORCID iD: 0000-0002-0114-3930
senior researcher, Department of Immunobiological Diagnostic Methods, Federal Scientific Center for Medical and Preventive Health Risk Management Technologies, Perm, 614045, Russian Federation
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