Health Сare of the Russian FederationHealth Сare of the Russian FederationЗдравоохранение Российской Федерации0044-197X2412-0723Federal Scientific Center for Hygiene F.F.Erisman63794610.46563/0044-197X-2020-64-6-351-357TOXICOLOGY (PREVENTIVE, CLINICAL, ECOLOGICAL)ТОКСИКОЛОГИЯ (ПРОФИЛАКТИЧЕСКАЯ, КЛИНИЧЕСКАЯ, ЭКОЛОГИЧЕСКАЯ)Oxidative carbonilation of liver tissue proteins under the influence of pesticide based on glyphosate in a subchronic experimentОкислительное карбонилирование белков ткани печени под воздействием пестицида на основе глифосата в субхроническом экспериментеhttps://orcid.org/0000-0001-5648-6669MiroshnikovaDarya I.МирошниковаДарья Игоревна
Russian Federation

MD, PhD-student of the Department of profile hygiene disciplines with a course of hygiene, epidemiology and organization of the state sanitary and epidemiological service, Ryazan State Medical University, Ryazan, 390026, Russia.

e-mail: d.i.miroshnikova9@gmail.com

Аспирант кафедры профильных гигиенических дисциплин с курсом гигиены, эпидемиологии и организации госсанэпидслужбы ФДПО ФГБОУ ВО РязГМУ Минздрава России, 390026, Рязань.

e-mail: d.i.miroshnikova9@gmail.com

d.i.miroshnikova9@gmail.comhttps://orcid.org/0000-0002-9959-6507RakitskiiValerii N.РакитскийВ. Н.
Russian Federation
https://orcid.org/0000-0001-5550-0625FominaMaria A.ФоминаМ. А.
Russian Federation
https://orcid.org/0000-0002-1258-9807KiryushinValerii A.КирюшинВ. А.
Russian Federation
https://orcid.org/0000-0003-0316-5479MotalovaTatiana V.МоталоваТ. В.
Russian Federation
Ryazan State Medical UniversityФГБОУ ВО «Рязанский государственный медицинский университет имени академика И.П. Павлова» Минздрава РоссииF.F. Erisman Federal Scientific Center for Hygiene of the Federal Service for Supervision in Protection of the Rights of Consumer and Man WellbeingФБУН «Федеральный научный центр гигиены имени Ф.Ф. Эрисмана» РоспотребнадзораPirogov Russian National Research Medical UniversityФГАОУ ВО «Российский национальный исследовательский медицинский университет имени Н.И. Пирогова» Минздрава России0101202164635135725102024Copyright ©; 2021, Miroshnikova D.I., Rakitskii V.N., Fomina M.A., Kiryushin V.A., Motalova T.V.Copyright ©; 2021, Мирошникова Д.И., Ракитский В.Н., Фомина М.А., Кирюшин В.А., Моталова Т.В.2021Miroshnikova D.I., Rakitskii V.N., Fomina M.A., Kiryushin V.A., Motalova T.V.Мирошникова Д.И., Ракитский В.Н., Фомина М.А., Кирюшин В.А., Моталова Т.В.https://creativecommons.org/licenses/by/4.0https://journals.eco-vector.com/0044-197X/article/view/637946

Introduction. Currently, the pathogenetic mechanisms of the action on the body of widely used glyphosate-based herbicides and the search for the markers of the health status that changes under the influence of these herbicides remain open for study and discussion. Carbonyl derivatives of proteins can be used as the markers of oxidative stress caused by the herbicidal composition of the isopropylamine salt of glyphosate.

The purpose of this study was to assess the severity of oxidative carbonylation of liver tissue proteins under the influence of a glyphosate-based pesticide in a subchronic experiment.

Material and methods. The material for the study was subcellular fractions of liver tissue homogenates obtained from 90 Wistar rats, which were administered solutions of glyphosate isopropylamine salt at doses of 280 mg/kg and 100 mg/kg per os for 3 months. The severity of oxidative carbonylation of proteins was determined by the method of R.L. Levine modified by E.E. Dubinina.

Results. Statistically significant changes in the content of products of oxidative damage to proteins at various stages of the experiment in the study groups were obtained compared with the control group due to a significant increase in the products of oxidative damage to proteins in subcellular fractions of liver tissue.

Conclusion. The changes in carbonyl stress indices and the decrease in the reserve-adaptive potential indicate the depletion of antioxidant protection in the liver cells. A statistically significant increase in secondary markers of carbonyl stress after 1 month from the start of seeding of animals compared with the control with a slight increase in this index after 3 months may indicate the launch of adaptation mechanisms, including the induction of proteolytic utilization of oxidized proteins or additional protein synthesis.

Введение. В настоящее время изучаются патогенетические механизмы действия на организм широко применяемых гербицидов на основе изопропиламинной соли (глифосата) и ведется поиск маркеров состояния здоровья, изменяющихся под воздействием этих веществ. В качестве маркеров окислительного стресса, вызванного гербицидным составом глифосата, могут использоваться карбонильные производные белков.

Целью данного исследования явилась оценка выраженности окислительного карбонилирования белков ткани печени под воздействием пестицида на основе глифосата в субхроническом эксперименте.

Материал и методы. Материалом для исследования явились субклеточные фракции гомогенатов ткани печени, полученные от 90 крыс Вистар, которым в течение 3 мес перорально вводили растворы глифосата в дозах 100 и 280 мг/кг. Выраженность окислительного карбонилирования белков определяли по методу R.L. Levine в модификации Е.Е. Дубининой.

Результаты. Получены статистически значимые изменения в содержании продуктов окислительного повреждения белков в субклеточных фракциях ткани печени на различных этапах эксперимента в группах исследования по сравнению с показателями контрольной группы.

Заключение. Изменения показателей карбонилового стресса и снижение значений показателя резервно-адаптационного потенциала свидетельствуют об истощении антиоксидантной защиты в клетках печени. Статистически значимое нарастание вторичных маркеров карбонилового стресса через 1 мес от начала затравки животных по сравнению с контролем при незначительном повышении этого показателя через 3 мес может свидетельствовать о запуске адаптационных механизмов, в том числе об индуцировании процессов протеолитической утилизации окисленных протеинов или дополнительного синтеза белка.

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