Human PhysiologyHuman PhysiologyФизиология человека0131-16463034-6150The Russian Academy of Sciences66398210.31857/S013116462210023XMJQLSUArticlesСтатьиEight-Week Aerobic Training Activates Extracellular Matrix Biogenesis in Human Skeletal MuscleВосьминедельная аэробная тренировка активирует биогенез внеклеточного матрикса в скелетной мышце человекаLednevE. M.ЛедневЕ. М.ledhauz@gmail.comLysenkoE. A.ЛысенкоЕ. А.ledhauz@gmail.comZgodaV. G.ЗгодаВ. Г.ledhauz@gmail.comGazizovaG. R.ГазизоваГ. Р.ledhauz@gmail.comShagimardanovaE. I.ШагимардановаЕ. И.ledhauz@gmail.comMakhnovskiiP. A.МахновскийП. А.ledhauz@gmail.comVinogradovaO. L.ВиноградоваО. Л.ledhauz@gmail.comDubrovV. E.ДубровВ. Э.ledhauz@gmail.comPopovD. V.ПоповД. В.ledhauz@gmail.comInstitute of Biomedical Problems of the RASФГБУН ГНЦ РФ – Институт медико-биологических проблем РАНInstitute of Biomedical ChemistryФГБНУ Научно-исследовательский институт биомедицинской химии имени В.Н. ОреховичаKazan Federal UniversityКазанский государственный университетMoscow State UniversityМосковский государственный университет имени М.В. Ломоносова01032023492445325022025Copyright ©; 2023, Е.М. Леднев, Е.А. Лысенко, В.Г. Згода, Г.Р. Газизова, Е.И. Шагимарданова, П.А. Махновский, О.Л. Виноградова, В.Э. Дубров, Д.В. ПоповCopyright ©; 2023, Е.М. Леднев, Е.А. Лысенко, В.Г. Згода, Г.Р. Газизова, Е.И. Шагимарданова, П.А. Махновский, О.Л. Виноградова, В.Э. Дубров, Д.В. Попов2023Е.М. Леднев, Е.А. Лысенко, В.Г. Згода, Г.Р. Газизова, Е.И. Шагимарданова, П.А. Махновский, О.Л. Виноградова, В.Э. Дубров, Д.В. ПоповЕ.М. Леднев, Е.А. Лысенко, В.Г. Згода, Г.Р. Газизова, Е.И. Шагимарданова, П.А. Махновский, О.Л. Виноградова, В.Э. Дубров, Д.В. Поповhttps://journals.eco-vector.com/0131-1646/article/view/663982

We aimed to investigate the effect of 8 weeks of moderate endurance training without considerable mechanical stress on the activation of extracellular matrix (ECM) gene expression in human skeletal muscle. Mechanical stress activates ECM biogenesis in the skeletal muscles, therefore only aerobic exercise on a cycling ergometer with concentric muscle contractions was used in the study. Skeletal muscle samples from m. vastus lateralis were taken from seven young untrained males before and after 8 weeks of aerobic training. Changes in the transcriptome (RNA sequencing) and proteome (shotgun quantitative proteomics analysis) were assessed in the samples; ECM-associated proteins (or matrisome) were determined using the Matrisome DB database. After training period, a change (mainly an increase) in the content of 14 ECM proteins and 134 mRNAs of ECM proteins was found. The largest increase in protein content was found for collagens 1 and 3 (1.7 and 2.2 times, respectively) – the main proteins of the human skeletal muscle’s ECM, which was consistent with an increase in the corresponding mRNA by 10–20 times. In addition, an increase in the expression of more than a hundred mRNAs of collagens, glycoproteins, proteoglycans, and enzymatic regulators of ECM was found, which occurs simultaneously with of an increase in the expression of genes of growth factors (IGF1, PDGFs, TGFB1, MDK, etc.) playing an important role in ECM biogenesis regulation. In conclusion, 8-week aerobic exercise training without considerable mechanical stress is a powerful stimulus for the activation of ECM biogenesis in skeletal muscle.

Целью работы было исследование влияния 8-недельных аэробных тренировок умеренной интенсивности без ударных воздействий на активацию экспрессии генов внеклеточного матрикса (ВКМ) в скелетной мышце. Механические ударные воздействия активируют биогенез ВКМ в скелетной мышце, поэтому в исследовании были использованы аэробные физические упражнения на велоэргометре, включающие только концентрические сокращения мышц. У семи молодых нетренированных мужчин были взяты пробы из m. vastus lateralis до и после 8-недельных аэробных тренировок. В пробах оценивали изменения транскриптома (РНК секвенирование) и протеома (панорамный количественный масс-спектрометрический анализ); белки, ассоциированные с ВКМ (объединенные общим термином “матрисом”), определяли по базе данных MatrisomeDB. После тренировки было обнаружено изменение (главным образом увеличение) содержания 14 белков ВКМ и 134 мРНК белков ВКМ. Наибольший прирост содержания белков найден для коллагенов 1 и 3 типа (1.7 и 2.2 раза, соответственно) – основные белки ВКМ скелетной мышцы человека, что согласовалось с увеличением соответствующих мРНК в 10–20 раз. Помимо этого, было найдено увеличение экспрессии более сотни мРНК коллагенов, гликопротеинов, протеогликанов и энзиматических регуляторов ВКМ, происходящее на фоне увеличения экспрессии генов основных ростовых факторов, регулирующих биогенез ВКМ (IGF1, PDGFs, TGFB1, MDK и др.). Таким образом, регулярные 8-недельные аэробные нагрузки без ударных воздействий на мышцы являются мощным стимулом для активации биогенеза ВКМ в скелетной мышце.

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