8 male volunteers aged 26 to 44 years were examined, who were in the “Arfa” hypomagnetic environment simulation facility for 4 hours at a normal magnetic field level (background session), and twice within 24 hours: one session during hypomagnetic environment simulation. environment with an attenuation coefficient of 500, and one − in the conditions of the terrestrial magnetic field (placebo). Venous blood was collected at the end of each session. The values of 48 biochemical parameters reflecting the state of organs and tissues, as well as the main links of metabolism, were determined in plasma and serum. Psychological testing of the testers was carried out using the questionnaires “Assessment of acute physical fatigue” by R. Kinsman and P. Weiser adapted by A.B. Leonova and “Assessment of acute mental fatigue” by A.B. Leonova and N.N. Savicheva. The level of physical condition of the participants of the experiment was assessed according to the method of D.N. Davidenko. As a result of the first biochemical screening examination and psychological testing of volunteers who were in a simulated space-level hypomagnetic environment, it was established that the effect is stressful, accompanied by the activation of glycolysis, lipolytic processes, affects the acid-base balance of the body, the state of the vascular endothelium, leads to to changes in the hepato-biliary system. At the same time, the index of physical fatigue among the volunteers increases, and the level of physical condition significantly decreases. Subjectively, pronounced changes in the biochemical parameters of stressful orientation are not felt.