Membrane and Cell BiologyMembrane and Cell BiologyБиологические мембраны0233-47553034-5219The Russian Academy of Sciences66746210.31857/S0233475524020064xppxnuArticlesСТАТЬИResearch ArticleInfluence of Low-Intense Laser Radiation He-Ne Laser on the Composition and Content of Phospholipids and Sterols in the Tissue of Wheat (Тriticum aestivum L.) Callus TissuesВлияние низкоинтенсивного лазерного излучения He-Ne-лазера на состав и содержание фосфолипидов и стеринов в тканях каллусов пшеницы Тriticum aestivum L.DudarevaL. V.ДудареваЛ. В.
Russian Federation
rudal69@mail.ruRudikovskayaE. G.РудиковскаяЕ. Г.
Russian Federation
rudal69@mail.ruSemenovaN. V.СеменоваН. В.
Russian Federation
rudal69@mail.ruRudikovskiiA. V.РудиковскийА. В.
Russian Federation
rudal69@mail.ruShmakovV. N.ШмаковВ. Н.
Russian Federation
rudal69@mail.ruSiberian Institute of Plant Physiology and Biochemistry of the Siberian Branch of the Russian Academy of SciencesСибирский институт физиологии и биохимии растений СО РАН1406202441214915926022025Copyright ©; 2024, The Russian Academy of SciencesCopyright ©; 2024, Российская академия наук2024The Russian Academy of SciencesРоссийская академия наукhttps://journals.eco-vector.com/0233-4755/article/view/667462

Using chromatography-mass spectrometry and thin-layer chromatography, the effect of irradiation with He-Ne laser light on the composition and content of cell membrane components – phospholipids and sterols – in wheat callus tissues was studied. It was shown that irradiation of callus with laser light at a dose of 3.6 J/cm2 led to significant changes in the content of these components. Thus, the content of phosphatidylinositol increased in irradiated callus by 8 times, phosphatidylethonolamine by 2 times, the content of phosphatidic acid decreased by 20% of the sum of phospholipids. For sterols, it was established that irradiation caused the most significant changes in the content of β-sitosterol, which is dominant in plants (an increase from 1453 ± 170 μg/g of dry weight in the non-irradiated control to 2001 ± 112 μg/g of dry weight 1 h after exposure) and, due to this, in the total content of sterols. Analysis of the results obtained suggests that phospholipids and sterols, primarily those for which regulatory and signaling functions are known, are involved in the response of plant tissue to exposure to low-intensity laser radiation from a He-Ne laser. This participation is realized as a stressful (nonspecific) response to intense radiation.

Методами хроматомасс-спектрометрии и тонкослойной хроматографии изучено влияние облучения светом He-Ne-лазера на состав и содержание компонентов клеточных мембран в каллусных тканях пшеницы: фосфолипидов (ФЛ) и стеринов. Показано, что облучение каллусов светом лазера в дозе 3.6 Дж/см2 приводило к значительным изменениям в содержании этих компонентов. Так, содержание фосфатидилинозита увеличилось в облученных каллусах в 8 раз, фосфатидилэтоноламина в 2 раза, содержание фосфатидной кислоты снизилось на 20% от суммы ФЛ. Для стеринов установлено, что наиболее существенные изменения облучение вызвало в содержании доминирующего в растениях β-ситостерина (увеличение с 1453±170 мкг/г сухого веса в необлученном контроле до 2001±111 мкг/г сухого веса через час после воздействия) и за счет этого в суммарном содержании стеринов. Анализ полученных результатов позволяет предположить, что ФЛ и стерины, в первую очередь те, для которых известны регуляторные и сигнальные функции, участвуют в реакции растительной ткани на воздействие низкоинтенсивным лазерным излучением He-Ne-лазера. Это участие реализуется как стрессовый (неспецифический) ответ на интенсивное излучение.

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