Sensory SystemsSensory SystemsСенсорные системы0235-00923034-5936The Russian Academy of Sciences67579010.31857/S0235009224010048ЗРИТЕЛЬНАЯ СИСТЕМАResearch ArticleNon-invasive recording of electroretinogram from both compound eyes in the cockroach Periplaneta americana L. in response to light stimuliНеинвазивная регистрация электроретинограммы от обоих сложных глаз у таракана Periplaneta americana L. в ответ на световые стимулыNovikovaE. S.НовиковаЕ. С.
Russian Federation
mzhukovskaya@rambler.ruAstakhovaL. A.АстаховаМ. Л.
Russian Federation
mzhukovskaya@rambler.ruRotovA. Y.РотовА. Ю.
Russian Federation
mzhukovskaya@rambler.ruZhukovskayaM. I.ЖуковскаяМ. И.
Russian Federation
mzhukovskaya@rambler.ruSechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of SciencesИнститут эволюционной физиологии и биохимии РАН21052024381526528022025Copyright ©; 2024, Russian Academy of SciencesCopyright ©; 2024, Российская академия наук2024Russian Academy of SciencesРоссийская академия наукhttps://journals.eco-vector.com/0235-0092/article/view/675790

The paper presents an original method of non-invasive registration of electroretinogram from both compound eyes of an insect. The method demonstrated high reliability and repeatability of the results. Using this method, it was shown that the magnitude of the light responses obtained from mutant cockroaches devoid of screening pigment, pearl, was about 4 times greater than those of wild-type insects. The time to peak of the response decreased with increasing light intensity, both for short-wavelength and long-wavelength stimuli. The pearl cockroaches exhibited a faster time to peak response than wild-type cockroaches; the results of covariance analysis indicate that these differences cannot be fully explained by an increase in the number of photons reaching the photoreceptor membranes and suggest additional differences in the compound eye physiology of mutant and wild-type insects. The positive voltage wave after the end of light stimulation depends on light intensity and reflects hyperpolarization of receptor cells. The photovoltaic effect, which distorts the amplitude and the shape of the response can be eliminated by using a gold wire as a recording electrode.

В работе представлен оригинальный метод неинвазивного отведения электроретинограммы от обоих сложных глаз насекомого. Метод показал высокую надежность и повторяемость результатов. Фотоэффект, искажающий амплитуду и форму ответа, был устранен использованием золотого отводящего электрода. С помощью этого метода показано, что величина ответов глаз мутантных тараканов pearl, лишенных экранирующего пигмента, на световой стимул примерно в четыре раза превышает ответы насекомых дикого типа. Время до пика ответа снижалось при увеличении интенсивности стимулирующего света для коротковолновых и длинноволновых стимулов. Тараканы pearl демонстрировали более быстрое, чем тараканы дикого типа, достижение максимума ответа. Результаты ковариационного анализа свидетельствуют, что эти различия не могут быть полностью объяснены увеличением числа фотонов, достигающих фоторецепторных мембран. Это предполагает дополнительные различия в физиологии сложного глаза мутантов и особей дикого типа. Положительное отклонение потенциала после окончания световой стимуляции зависело от интенсивности света и отражало гиперполяризацию рецепторных клеток.

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