Sensory SystemsSensory SystemsСенсорные системы0235-00923034-5936The Russian Academy of Sciences67580510.31857/S0235009223030071ZYGYYZСЛУХОВАЯ И ВЕСТИБУЛЯРНАЯ СИСТЕМЫEffect of octopamine on the frequency tuning of the auditory system in Culex pipiens pipiens mosquito (Diptera, Culicidae)Влияние октопамина на частотную настройку слуховой системы комаров Culex pipiens pipiens (Diptera, Culicidae)VorontsovD. D.ВоронцовД. Д.d.vorontsov@idbras.ruLapshinD. N.ЛапшинД. Н.lapshin@iitp.ruKoltzov Institute of Developmental Biology Russian Academy of SciencesИнститут биологии развития им. Н.К. Кольцова РАНInstitute for Information Transmission Problems of the Russian Academy of Sciences (Kharkevich Institute)Институт проблем передачи информации им. А.А. Харкевича РАН0107202337324425728022025Copyright ©; 2023, Russian Academy of SciencesCopyright ©; 2023, Российская академия наук2023Russian Academy of SciencesРоссийская академия наукhttps://journals.eco-vector.com/0235-0092/article/view/675805

We aimed at demonstrating the direct physiological effects of octopamine on the auditory responses of the Johnston’s organ sensory neurons in Culex pipiens pipiens mosquitoes. Responses to acoustic stimulation were measured as the frequency-threshold curves and as an instantly recorded autoexcitation frequency before and after the injection of octopamine (or chlordimeform, an agonist of octopamine receptors). The autoexcitation frequency was assessed by including the auditory neurons into a positive feedback loop, when the amplified neuronal response was used to drive the stimulating loudspeaker. Our results indicate that: octopamine affects the properties of the auditory system both in male and female mosquitoes; in female mosquitoes, octopamine significantly reduces the sensitivity of auditory neurons below 90 Hz and produces little effect in the high-frequency range (100 Hz and above); in male mosquitoes, octopamine significantly raises the tuning frequency of the auditory system (ratio of tuning frequencies before and after the injection 1.32–1.55). There is a strong sexual dimorphism in the octopaminergic modulation of the mosquito auditory system. The observed physiological effects of octopamine, both in male and female mosquitoes, cannot be fully explained by the modulation of mechanical stiffness of the antenna and must include the changes in frequency tuning of the auditory neurons.

Цель работы состояла в демонстрации физиологических эффектов действия октопамина на слуховые ответы сенсорных нейронов джонстонова органа у комаров Culex pipiens pipiens L. Ответы нейронов на звуковую стимуляцию измеряли до и после введения октопамина (а также хлордимеформа, агониста октопаминовых рецепторов) в виде частотно-пороговых кривых или регистрируемой в реальном времени частоты автовозбуждения, возникавшей при включении слухового нейрона в контур обратной связи с использованием усиленного ответа этого нейрона в качестве сигнала для возбуждения акустического излучателя. Из наших результатов следует: октопамин влияет на свойства слуховой системы как самцов, так и самок комаров; у самок комаров октопамин значительно снижает чувствительность рецепторов в диапазоне частот ниже 90 Гц и слабо влияет на чувствительность в диапазоне от 100 Гц и выше; у самцов комаров октопамин существенно повышает частотную настройку слуховой системы (соотношение частот после и до введения вещества 1.32–1.55). Показан сильный половой диморфизм в октопаминергической модуляции слуховой системы комаров. Наблюдаемые физиологические эффекты октопамина как у самцов, так и у самок комаров не могут быть полностью объяснены модуляцией механической жесткости антенны и должны включать изменение оптимальных частот настройки слуховых нейронов.

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