Sensory SystemsSensory SystemsСенсорные системы0235-00923034-5936The Russian Academy of Sciences67582010.31857/S0235009223010043ATSRWQОБОНЯТЕЛЬНАЯ СИСТЕМАChanges in the spectral characteristics and the coherence of the rat olfactory bulb local field potentials under xylazine-tiletamine-zolazepam anesthesiaИзменение спектральных характеристик и уровня когерентности фокальной активности обонятельной луковицы крысы в динамике ксилазин-тилетамин-золазепамового наркозаKiroyV. N.КиройВ. Н.peza-i@mail.ruKosenkoP. O.КосенкоП. О.peza-i@mail.ruShaposhnikovP. D.ШапошниковП. Д.peza-i@mail.ruSaevskiyA. I.АсланянЕ. В.peza-i@mail.ruAslanyanE. V.СаевскийА. И.peza-i@mail.ruSouthern Federal UniversityЮжный федеральный университет01012023371657728022025Copyright ©; 2023, Russian Academy of SciencesCopyright ©; 2023, Российская академия наук2023Russian Academy of SciencesРоссийская академия наукhttps://journals.eco-vector.com/0235-0092/article/view/675820

Local field potentials (LFP) recorded in the olfactory bulb (OB) are known to be largely generated in local neural networks, are directly related to the processing of olfactory information, and are influenced by various factors, including anesthetics. Using 8-electrode arrays implanted in the dorsal regions of 6 adult male Norway rats OBs, the effects of xylazine-tiletamine-zolazepam (XTZ) anesthesia on the spectral characteristics and coherence of the LFP in the frequency range 1–150 Hz were studied in 2 hours long chronic experiments. It is shown that the most significant changes in the rat OB LFP under XTZ-anesthesia are observed in the high γ frequency band. A statistically significant increase in the power (2–4 times) and coherence (up to 50%) of this band was observed in the LFP of all animals within 10–15 minutes from the anesthesia onset. At the same time, during wakefulness, the dominant frequencies of this band were 70–80 Hz, and within 10–15 minutes from the anesthesia onset they increased to 110–130 Hz. During anesthesia, a gradual shift of these dominant frequencies to a lower range (90–110 Hz) was observed, while their total power, in contrast to the coherence, was statistically significantly reduced when the animal started recovering from anesthesia

Известно, что фокальная активность (ФА), регистрируемая в обонятельной луковице (ОЛ), в значительной степени генерируется в ее локальных нейронных сетях, имеет непосредственное отношение к обработке ольфакторной информации и подвержена влиянию различных факторов, в том числе анестетиков. С использованием 8-электродных матриц на шести взрослых самцах серых доминитицированных крыс в хронических экспериментах исследовали эффекты ксилазин-тилетамин-золазепамовой (КТЗ) анестезии на спектральные характеристики и когерентность ФА, регистрируемой с дорзальной поверхности ОЛ в частотном диапазоне 1–150 Гц в течение двух часов. Показано, что наиболее существенные изменения в ФА ОЛ крыс в КТЗ-наркозе наблюдаются в области высоких гамма-частот. Статистически значимое увеличение мощности (в 2–4 раза) и когерентности (до 50%) этих частот наблюдалось в ФА всех животных уже в течение 10–15 мин от начала наркотизации. При этом, если в бодрствовании модальные значения этих частот приходились на область 70–80 Гц, то по прошествии указанного времени – 110–130 Гц. В динамике наркоза наблюдалось постепенное смещение модального значения в распределении их мощности влево, в область более низких частот (90–110 Гц), при этом их суммарная мощность (но не когерентность) статистически значимо снижалась лишь на фоне выхода животного из наркоза.

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