Comparative analysis of blood and follicular fluid lipid profiles in women undergoing infertility treatment withassisted reproductive technologies
- Authors: Fortygina Y.A.1, Makarova N.P.1, Drapkina Y.S.1, Novoselova A.V.1, Gamisonia A.M.1, Chagovets V.V.1, Frankevich V.E.1,2, Kalinina E.A.1
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Affiliations:
- Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation
- Siberian State Medical University of the Ministry of Health of Russia
- Issue: No 4 (2024)
- Pages: 93-102
- Section: Original Articles
- URL: https://journals.eco-vector.com/0300-9092/article/view/632102
- DOI: https://doi.org/10.18565/aig.2024.62
- ID: 632102
Cite item
Abstract
Background: Follicle fluid (FF) composition can significantly affect oocyte development, fertilization, and early embryo cleavage. Therefore, studying the molecular composition of FF can provide valuable insights into the mechanisms and factors that influence oogenesis. Investigating the lipid profile of FF as an additional marker for assessing oocyte quality has shown promising results. However, FF collection is an invasive procedure; therefore, it is important to explore indirect sources of information about FF composition.
Objective: To compare the lipid profiles of blood plasma and FF in women undergoing infertility treatment using assisted reproductive technology (ART).
Materials and methods: The study involved 40 married couples, aged 24–39 years, with a normal body mass index (up to 25 kg/m2), seeking infertility treatment with ART. Patients underwent ovarian stimulation following a protocol with gonadotropin-releasing hormone antagonists (GnRH antagonists). On the day of the puncture, FF and blood plasma were collected and cryopreserved. Liquid chromatography with mass spectrometry was used to determine the molecular compositions of the samples.
Results: The study determined the lipid composition of FF (175 lipids) and blood plasma (185 lipids). Among these molecules, 70 lipids were identical in both FF and blood plasma. Of these, 42 lipids showed a statistically significant correlation. Additionally, when analyzing the correlation between plasma lipid levels and FF from the left and right ovaries, 25 lipids were identified, with plasma levels significantly correlated with FF from the left ovary, and 40 lipids showed a significant correlation with FF from the right ovary. The levels of all 175 identified lipids showed a statistically significant correlation between the left and right ovarian FF.
Conclusion: These findings suggest that the similarities and differences found between blood plasma and FF lipidomes can be used to develop noninvasive methods for assessing oocyte status and predicting the effectiveness of ART. The study results suggest the potential for personalized infertility treatment and preparation for ART programs, as well as a deeper understanding of the mechanisms underlying impaired oocyte maturation and the causes of low fertilization rates.
Keywords
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About the authors
Yulia A. Fortygina
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation
Author for correspondence.
Email: yu_fortygina@oparina4.ru
ORCID iD: 0000-0002-1251-0505
graduate student
Russian Federation, MoscowNatalya P. Makarova
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation
Email: np_makarova@oparina4.ru
Dr. Bio. Sci., Leading Researcher, Department of IVF named after Professor B.V. Leonov
Russian Federation, MoscowYulia S. Drapkina
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation
Email: yu_drapkina@oparina4.ru
ORCID iD: 0000-0002-0545-1607
PhD, Researcher, Department of IVF named after Professor B.V. Leonov
Russian Federation, MoscowAnastasia V. Novoselova
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation
Email: a_novoselova@oparina4.ru
Researcher, Laboratory of Metabolomics and Bioinformatics
Russian Federation, MoscowAlina M. Gamisonia
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation
Email: a_gamisoniya@oparina4.ru
ORCID iD: 0000-0001-8532-4714
Researcher, Laboratory of Metabolomics and Bioinformatics
Russian Federation, MoscowVitaly V. Chagovets
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation
Email: vvchagovets@gmail.com
ORCID iD: 0000-0002-5120-376X
PhD, Deputy Director of the Institute of Translational Medicine
Russian Federation, MoscowVladimir E. Frankevich
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation; Siberian State Medical University of the Ministry of Health of Russia
Email: v_frankevich@oparina4.ru
ORCID iD: 0000-0002-9780-4579
Dr. Sci. in physics and mathematics, Deputy Director of the Institute of Translational Medicine
Russian Federation, Moscow; TomskElena A. Kalinina
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation
Email: e_kalinina@oparina4.ru
ORCID iD: 0000-0002-8922-2878
Dr. Med. Sci., Professor, Head of the IVF Department named after Professor B.V. Leonov
Russian Federation, MoscowReferences
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