Obstetrics and GynecologyObstetrics and GynecologyАкушерство и гинекология0300-90922412-5679Bionika Media24794410.18565/aig.2017.3.69-74ArticlesСтатьиResearch ArticleImpact of sperm DNA fragmentation on the efficiency of fertilization and the development of human embryos cultured in vitroВлияние фрагментации ДНК сперматозоидов на эффективность оплодотворения и развитие эмбрионов человека, культивируемых in vitroMazilinaMaria A.МазилинаМария Алексеевна

a graduate student; embryologist of the ART department

аспирант; эмбриолог отделения ВРТ

mashamazilina@gmail.comKomarovaEvgenia M.КомароваЕвгения Михайловна

PhD, junior researcher, embryologist of the ART department

к.б.н., м.н.с., эмбриолог отделения ВРТ

evgmkomarova@gmail.comLesikElena A.ЛесикЕлена Александровна

PhD, senior researcher, embryologist of the ART department

к.б.н., с.н.с., эмбриолог отделения ВРТ

lesike@yandex.ruFedorovaIrina D.ФедороваИрина Дмитриевна

PhD, senior researcher, a senior embryologist

к.б.н., с.н.с., старший эмбриолог

irendf@mail.ruGzgzyanAlexander M.ГзгзянАлександр Мкртичевич

prof. of the Department of Obstetrics and Gynecology; Head of the Department of Obstetrics

д.м.н., проф. кафедры акушерства и гинекологии; руководитель отделения ВРТ

agzgzyan@mail.ruSaint Petersburg State UniversityФГБОУ ВПО Санкт-Петербургский государственный университетD.O. Ott Research Institute of Obstetrics, Gynecology, and ReproductologyФГБНУ НИИ акушерства, гинекологии и репродуктологии им. ДО. ОттаD.O. Ott Research Institute of Obstetrics, Gynecology, and ReproductologyФГБНУ НИИ акушерства, гинекологии и репродуктологии им. Д О. Отта150320173NO3 (2017)№3 (2017)697418022023Copyright ©; 2017, Bionika MediaCopyright ©; 2017, ООО «Бионика Медиа»2017Bionika MediaООО «Бионика Медиа»https://journals.eco-vector.com/0300-9092/article/view/247944

Objective. To evaluate the impact of sperm DNA fragmentation on the efficiency of fertilization and the development of human embryos cultured in vitro. Material and methods. The data of oocyte fertilization and embryo production protocols were analyzed. Semen analysis was performed according to the 2010 WHO recommendations. The proportion of sperm with fragmented DNA was analyzed by fluorescently labeling of DNA strand breaks (TUNEL). Statistical analysis was carried out using GraphPad InStat software. Results. There was a negative correlation between the proportion of sperm with fragmented DNA and the efficiency of fertilization. No correlation was found between the proportion of sperm with fragmented DNA and that of high-quality embryos. Conclusion. Sperm DNA fragmentation has been shown to negatively affect the efficiency of fertilization; however, it does not impair the early preimplantation development of embryos when cultured in vitro.

Цель исследования. Оценить влияние фрагментации ДНК сперматозоидов на эффективность оплодотворения и развитие эмбрионов человека, культивируемых in vitro. Материал и методы. Проведен анализ данных протоколов оплодотворения яйцеклеток и культивирования эмбрионов. Спермиологический анализ выполнен согласно рекомендациям ВОЗ (WHO, 2010). Доля сперматозоидов с фрагментированной ДНК проанализирована методом флуоресцентного мечения разрывов ДНК (TUNEL). Статистический анализ был проведен с помощью программного обеспечения GraphPad InStat. Результаты. Между долей сперматозоидов с фрагментированной ДНК и эффективностью оплодотворения была выявлена отрицательная взаимосвязь. Не было обнаружено корреляции между долей сперматозоидов с фрагментированной ДНК и долей эмбрионов высшего качества. Заключение. Показано, что фрагментация ДНК сперматозоидов негативно отражается на эффективности оплодотворения, однако не приводит к нарушению раннего доимплантационного развития эмбрионов при культивировании in vitro.

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