Features of changes in the monocyte-macrophage cells in the placenta during preeclampsia


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Abstract

Objective: To determine the features of changes in the cells of the monocytic-macrophage link in the placenta with preeclampsia of vary ing severity. Materials and methods: The study included 52 patients. Women were divided into the main group with preeclampsia (n=26) and the comparison group - physiological pregnancy (n=26). The main group was divided into two subgroups with mild (n=12) and severe (n=14) preeclampsia. The content of CD68+ cells inside the placental villi was studied. The study was carried out using immunohistochemistry, which was confirmed by Western blotting. Results: It was found that the content of CD68+ cells inside the placental villi was significantly higher in the groups of mild -7,5% (5.6; 8.3) and severe - 9,1% (6.5; 10.8) preeclampsia relative to the comparison group - 5,1% (2.5; 5.3) (p<0.001). The relative level of expression of the CD68 protein, assessed by the Western blot method, was also statistically significantly increased in PE. Conclusion: When studying CD68+ cells in placental villi, a correlation was found between an increase in their content and the severity of preeclampsia. Thus, the results obtained may indicate a potential role of the placenta in the pathogenesis of PE as an agent activating cytotoxic properties in maternal blood monocytes.

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About the authors

Daiana A. Boris

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: dayana_boris@mail.ru
PhD, Researcher

Victor L. Tyutyunnik

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Dr. Med. Sci., Professor, Leading Researcher of Research and Development Service

Natalia E. Kan

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: kan-med@mail.ru
Dr. Med. Sci., Professor, Deputy Director of Science

Aleksandr I. Shchegolev

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: ashegolev@oparina4.ru
Dr. Med. Sci., Professor, Head of the Department of Morbid Anatomy

Veronica A. Sinitsyna

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: v_sinitsyna@oparina4.ru
senior laboratory assistant, Pathology Department

Alsu A. Sadekova

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: a_sadekova@oparina4.ru
PhD, Scientific researcher of the Cytology Laboratory

Aleksey M. Krasnyi

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: alexred@list.ru
PhD, Head of the Cytology Laboratory

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