Production of reactive oxygen species by leukocytes stimulated with zymosan and lipopolysaccharide in women with miscarriage in early pregnancy


Дәйексөз келтіру

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Ашық рұқсат Ашық рұқсат
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Аннотация

Objective: To study the production of reactive oxygen species by polymorphonuclear leukocytes in peripheral blood stimulated with zymosan and lipopolysaccharide, which are recognized by Toll-like receptors in women with miscarriage associated with infection in early pregnancy compared to healthy pregnant women. Materials and methods: 75 women aged 18-42 years were tested. They were stratified into 3 groups: group 1 - healthy nonpregnant women (n=25); group 2 - healthy pregnant women (n=25); group 3 - women with miscarriage associated with infection (n=25). In all women, the term of pregnancy was the first trimester of gestation (5-12 weeks). The functional activity of polymorphonuclear leukocytes in peripheral blood was determined by luminol-dependent chemiluminescence. Results: It was found that the production of reactive oxygen species by polymorphonuclear leukocytes stimulated with zymosan and lipopolysaccharide increased from 5 to 100 ig/mL, and the production of reactive oxygen species by the cells stimulated with lipopolysaccharide decreased from 50 to 100 ig/mL compared to healthy pregnant women. Conclusion: In women with miscarriage associated with infections and in healthy pregnant women on the background of oxidative stress in the first trimester of pregnancy, the production of reactive oxygen species by polymorphonuclear leukocytes in peripheral blood stimulated with zymosan or lipopolysaccharide, which are recognized by Toll-like receptors, may depend on the expression of these receptors on the membrane of cells.

Толық мәтін

Рұқсат жабық

Авторлар туралы

Bayarma Zhambalova

N.I. Pirogov Russian National Research Medical University of the Ministry of Health of Russia

Email: zhambalovaba@inbox.ru
Ph.D., Department of Medical Biophysics

Anatoliy Osipov

N.I. Pirogov Russian National Research Medical University of the Ministry of Health of Russia

Email: anosipov@yahoo.com
Dr. Bio. Sci., Professor, Corresponding Member of the RAS, Head of the Department of General and Medical Biophysics of the Faculty of Medicine and Biology, Head of the Department of Medical Biophysics, Research Institute of Translational Medicine

Yuriy Vladimirov

N.I. Pirogov Russian National Research Medical University of the Ministry of Health of Russia

Dr. Bio. Sci., Academician of the RAS, Professor, Department of General and Medical Biophysics of the Faculty of Medicine and Biology

Yuliya Dobrokhotova

N.I. Pirogov Russian National Research Medical University of the Ministry of Health of Russia

Honored Doctor of the Russian Federation, Dr. Med. Sci., Professor, Head of the Department of Obstetrics and Gynecology of the Medical Faculty

Lyudmila Gankovskaya

N.I. Pirogov Russian National Research Medical University of the Ministry of Health of Russia

Dr. Med. Sci., Professor, Head of the Department of Immunology of the Faculty of Medicine and Biology

Yuriy Teselkin

N.I. Pirogov Russian National Research Medical University of the Ministry of Health of Russia

Dr. Bio. Sci., Chief Researcher of the Department of Medical Biophysics, Research Institute of Translational Medicine

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