The evaluation of the effect of Epigen intim spray on bacterial biofilms formed by vaginal microorganisms in vitro

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Аннотация

Objective: To evaluate the effect of 0.1% Epigen Intim spray in vitro on bacterial biofilms formed by vaginal microorganisms.

Materials and methods: This was a study of 72 clinical isolates of pure cultures of microorganisms obtained from the vaginal biotope: G. vaginalis (3 isolates), E. faecalis (9 isolates), E. coli (18 isolates), K. pneumoniae (15 isolates), K. aerogenes (3 isolates), L. crispatus (3 isolates), S. pyogenes (3 isolates), A. baumannii (3 isolates), S. aureus (3 isolates), C. albicans (3 isolates), E. faecium (3 isolates), S. agalactiae (3 isolates), L. acidophilus (3 isolates). Dense and liquid selective culture media were used for cultivation, storage and further research. Microorganisms were identified using latex agglutination and MALDI-TOF mass spectrometry (Bruker Microflex). The ability to form biofilms was evaluated using a modified version of a protocol developed by Christensen et al. (1985). In order to assess the effect on the bacterial biofilms, 0.1% Epigen Intim spray containing activated glycyrrhizic acid (0.1 g per 100 ml) was used.

Results: Among 72 clinical isolates of vaginal microorganisms, 38 demonstrated the ability to form bacterial biofilms (G. vaginalis, K. pneumoniae, E. coli, E. faecium). The tested clinical isolates of L. crispatus, L. acidophilus, C. albicans, S. agalactiae, S. pyogenes and others (34 isolates out of 72) did not form biofilms. Thus, 53% of the microorganisms inhabiting the vaginal biotope were able to form biofilms. These clinical isolates of microorganisms were included in the study. Epigen Intim spray showed high efficacy against 34 out of 38 (89.48%) isolates of microorganisms which form biofilms. The biofilms formed by 4 isolates of microorganisms (2 isolates of K. pneumoniae, E. coli and E. faecalis) were not affected by 0.1% Epigen Intim spray.

Conclusion: The biofilms formed by vaginal microorganisms such as G. vaginalis, K. pneumoniae, E. coli, E. faecium are destroyed by 0.1% Epigen Intim spray, containing glycyrrhizic acid with an efficiency of 89.48% in vitro. It should be noted that a decrease in the optical density of the biofilm by more than 3 times indicates the biofilm-destroying effect of the medication. The ratio of change in optical density after exposure to 0.1% Epigen Intim spray ranged from 3.66 to 743 for 34 isolates of microorganisms.

Толық мәтін

Рұқсат жабық

Авторлар туралы

Kira Shalepo

D.O. Ott Research Institute of Obstetrics, Gynecology and Reproduction; St. Petersburg State Pediatric Medical University, Ministry of Health of Russia

Хат алмасуға жауапты Автор.
Email: 2474151@mail.ru
ORCID iD: 0000-0002-3002-3874

PhD, MD, Senior Researcher, Experimental Microbiology Group, Associate Professor, Department of Clinical Laboratory Diagnostics of AF and DPO, Senior Researcher, International Center for the Study of the Vitality and Resistance of Polymicrobial Communities and Biofilms

Ресей, 199034, St. Petersburg, Mendeleevskaya line, 3; 194100, Russia, St. Petersburg, Litovskaya str., 2

Elena Spasibova

D.O. Ott Research Institute of Obstetrics, Gynecology and Reproduction; St. Petersburg State Pediatric Medical University, Ministry of Health of Russia

Email: elena.graciosae@gmail.com
ORCID iD: 0009-0002-6070-4651

bacteriologist, Laboratory of Clinical Microbiology, Assistant, Department of Clinical Laboratory Diagnostics, bacteriologist, International Center for the Study of the Vitality and Resistance of Polymicrobial Communities and Biofilms

Ресей, 199034, St. Petersburg, Mendeleevskaya line, 3; 194100, Russia, St. Petersburg, Litovskaya str., 2

Olga Budilovskaya

D.O. Ott Research Institute of Obstetrics, Gynecology and Reproduction; St. Petersburg State Pediatric Medical University, Ministry of Health of Russia

Email: o.budilovskaya@gmail.com
ORCID iD: 0000-0001-7673-6274

PhD, MD, Senior Researcher, Experimental Microbiology Group, Assistant, Department of Clinical Laboratory Diagnostics of AF and DPO, PhD, MD, Senior Researcher, International Center for the Study of the Vitality and Resistance of Polymicrobial Communities and Biofilms

Ресей, 199034, St. Petersburg, Mendeleevskaya line, 3; 194100, Russia, St. Petersburg, Litovskaya str., 2

Anna Krysanova

D.O. Ott Research Institute of Obstetrics, Gynecology and Reproduction; St. Petersburg State Pediatric Medical University, Ministry of Health of Russia

Email: krusanova.anna@mail.ru
ORCID iD: 0000-0003-4798-1881

PhD, MD, Researcher, Experimental Microbiology Group, Assistant, Department of Clinical Laboratory Diagnostics, PhD, MD, Researcher, International Center for the Study of the Vitality and Resistance of Polymicrobial Communities and Biofilms

Ресей, 199034, St. Petersburg, Mendeleevskaya line, 3; 194100, Russia, St. Petersburg, Litovskaya str., 2

Tatiana Khusnutdinova

D.O. Ott Research Institute of Obstetrics, Gynecology and Reproduction; St. Petersburg State Pediatric Medical University, Ministry of Health of Russia

Email: husnutdinovat@yandex.ru
ORCID iD: 0000-0002-2742-2655

PhD, MD, Senior Researcher, Experimental Microbiology Group, Assistant, Department of Clinical Laboratory Diagnostics, PhD, MD, Senior Researcher, International Center for the Study of the Vitality and Resistance of Polymicrobial Communities and Biofilms

Ресей, 199034, St. Petersburg, Mendeleevskaya line, 3; 194100, Russia, St. Petersburg, Litovskaya str., 2

Alevtina Savicheva

D.O. Ott Research Institute of Obstetrics, Gynecology and Reproduction; St. Petersburg State Pediatric Medical University, Ministry of Health of Russia

Email: savitcheva@mail.ru
ORCID iD: 0000-0003-3870-5930

Dr.Med. Sci., Professor, Head of the Department of Medical Microbiology, Head of the Department of Clinical Laboratory Diagnostics, Head of the International Center for the Study of the Vitality and Resistance of Polymicrobial Communities and Biofilms

Ресей, 199034, St. Petersburg, Mendeleevskaya line, 3; 194100, Russia, St. Petersburg, Litovskaya str., 2

Alexandra Cheberya

D.O. Ott Research Institute of Obstetrics, Gynecology and Reproduction
St. Petersburg;S.M. Kirov Military Medical Academy

Email: alexa-vorobjeva.09@yandex.ru
ORCID iD: 0009-0008-1091-5753

laboratory assistant researcher at Microbiology Laboratory, 5th year student

Ресей, 199034, St. Petersburg, Mendeleevskaya Line, 3; 194044, St. Petersburg, Academician Lebedev str., 37

Alexander Cheberya

D.O. Ott Research Institute of Obstetrics, Gynecology and Reproduction
St. Petersburg;S.M. Kirov Military Medical Academy

Email: sanekcheberya@yandex.ru
ORCID iD: 0009-0006-9058-6720

laboratory assistant researcher at Microbiology Laboratory, 5th year student

Ресей, 199034, St. Petersburg, Mendeleevskaya Line, 3; 194044, St. Petersburg, Academician Lebedev str., 37

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