ASSESSMENT OF THE ACROSOME REACTION IN THE DIAGNOSIS OF INFERTILITY


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Resumo

Flow cytometric analysis of the sperm acrosome reaction enables one to effectively diagnose infertility caused by sperm dysfunctions in the presence of normal spermogram readings.

Sobre autores

M NIKOLAEVA

E GOLUBEVA

I UShAKOVA

L VAN'KO

V BOZhEDOMOV

G SUKhIKh

M Nikolaeva

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health and Social Development of the Russian Federation, Moscow

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health and Social Development of the Russian Federation, Moscow

Ye Golubeva

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health and Social Development of the Russian Federation, Moscow

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health and Social Development of the Russian Federation, Moscow

I Ushakova

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health and Social Development of the Russian Federation, Moscow

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health and Social Development of the Russian Federation, Moscow

L Vanko

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health and Social Development of the Russian Federation, Moscow

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health and Social Development of the Russian Federation, Moscow

V Bozhedomov

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health and Social Development of the Russian Federation, Moscow

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health and Social Development of the Russian Federation, Moscow

G Sukhikh

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health and Social Development of the Russian Federation, Moscow

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health and Social Development of the Russian Federation, Moscow

Bibliografia

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  2. Сухих Г.Т., Божедомов В.А. Мужское бесплодие. - М., 2009.
  3. Collins J.A., Barnhart K.T., Schlegel P.N. Do sperm DNA integrity tests predict pregnancy with in vitro fertilization? // Fertil. and Steril. - 2008. - Vol. 89, № 4. - P. 823-831.
  4. Cummins J.M., Pember S.M., Jequier A.M. A test of the human sperm acrosome reaction following ionophore challenge. Relationship to fertility and other seminal parameters. // J. Androl. - 1991. - Vol. 12, № 2. - P. 98-103.
  5. Guzick D.S., Overstreet J.W., Factor-Litvak P. Sperm morphology, motility, and concentration in fertile and infertile men. // N. Engl. J. Med.- 2001. - Vol. 345, № 19. - P. 1388-1393.
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  9. Nikolaeva M.A., Balyasnikova I. V., Alexinskaya M.A. Testicular isoform of angiotensin I-converting enzyme (ACE, CD143) on the surface of human spermatozoa: revelation and quantification using monoclonal antibodies. // Am. J. Reprod. Immunol. - 2006. - Vol. 55, № 1. - P. 54-68.
  10. Nikolaeva M.A., Golubeva E.L., Kulakov V.I. Evaluation of stimulus-induced acrosome reaction by two-colour flow cytometric analysis. // Mol. Hum. Reprod. - 1998. - Vol. 4, № 3. - P. 243-250.
  11. Pauls K., Metzger R., Steger K. Isoforms of angiotensin I-converting enzyme in the development and differentiation of human testis and epididymis. // Androl. - 2003. - Vol. 35, № 1. - P. 32-43.
  12. Practice Committee of American Society for Reproductive Medicine. // The clinical utility of sperm DNA integrity testing. Fertil. and Steril. - 2008. - Vol. 90, № 5. - P. 178-180.
  13. Tesarik J. Acrosome reaction testing. // Report of the consensus workshop on advanced diagnostic andrology techniques. ESHRE, Andrology Special Interest Group Hum. Reprod. - 1996. - Vol. 11. - P. 1463-1479.
  14. WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction. WHO, 4-th ed. - Cambridge., 1999.
  15. Zweig M.H., Campbell G. Receiver-operating characteristic (ROC) plots: a fundamental evaluation tool in clinical medicine.// Clin. Chemistr. - 1993. - Vol. 39. - P. 561-577.

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