ULTRASTRUCTURAL PATHOLOGY OF OOCYTES IN IN-VITRO FERTILIZATION CYCLES AS A CAUSE OF FEMALE INFERTILITY


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Resumo

The quality of used gametes (both sperm and oocytes) serves as a key factor of successful in vitro fertilization programs. The first stages of early human embryogenesis largely depend on the state of an oocyte. The process of female sex cell maturation is a chain of complex rearrangements of nuclear and cytoplasmic structures. There is accumulation of energy and nutrients, which are needed at the early stages of zygotic division and cleavage. That is the reason that oocytic dysmorphisms found at the light microscope level are frequently associated with internal cell pathology. The paper gives an update on the structure of human oocyte at the metaphase II stage according to electron microscopic data. It also describes the anecdotal cases suggesting that the cause of female (commonly idiopathic) infertility may be oocyte abnormality identified when implementing in vitro fertilization programs.

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Sobre autores

Natalya MAKAROVA

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: np.makarova@gmail.com
PhD, Researcher of the Department of in vitro fertilization 117997, Russia, Moscow, Ac. Oparin str. 4

Liya KAZARYAN

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: l_kazaryan@oparina4.ru
MD, PhD, Researcher of the Department of in vitro fertilization 117997, Russia, Moscow, Ac. Oparin str. 4

Evgeniya PETROVA

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: evgesha_petrova@mail.ru
Embryologist of the Department of in vitro fertilization 117997, Russia, Moscow, Ac. Oparin str. 4

Elena KULAKOVA

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: e_kulakova@oparina4.ru
MD, PhD, Researcher of the Department of in vitro fertilization 117997, Russia, Moscow, Ac. Oparin str. 4

Lubov KURILO

Medical Genetics Research Center, Russian Academy of Medical Sciences

Email: genreprlab@yandex.ru
MD., PhD, Professor, Head of Laboratory of reproduction genetics 115478, Russia, Moscow, Moskvoreche str. 1

Galina BARANOVA

All-Russian Research Institute of Agricultural Biotechnology, Russian Academy of Agricultural Sciences

Email: salex42@mail.ru
Researcher of Electron microscopy Laboratory 127550, Russia, Moscow, Timiryazev str

Vladimir POLYAKOV

All-Russian Research Institute of Agricultural Biotechnology, Russian Academy of Agricultural Sciences

Email: polyakovvladimir@mail.ru
MD., PhD., Professor, Electron microscopy Laboratory 127550, Russia, Moscow, Timiryazev str

Elena KALININA

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: e_kalinina@oparina4.ru
MD., PhD., Head of the Department of in vitro fertilization 117997, Russia, Moscow, Ac. Oparin str. 4

Bibliografia

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