Immunological parameters in pregnant women in the early prognosis of development of fetoplacental insufficiency


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Abstract

The authors studied cell subpopulations (CD4+CD25+ and CD4+CD25highT-reg; CD56+CD16- and CD56+CD16+NK cells; CD56+CD3+NKT cells) by flow cytofluorimetry and determined the biological (suppressor) activity of serum factors and the spontaneous production of cytokines (IFN-г, TNF-б, GM-CSF, IL-2, IL-4, IL-5, IL-10, IL-12, IL-13) in 24-hour whole blood cultures by multiplex proteomic analysis in 19 women in whom the course of pregnancy was complicated by the development with fetoplacental insufficiency (FPI) and in 13 women with physiological pregnancy, who were matched for age (30.2±0.8 and 32.3±0.6 years) and gestational age (10.8±0.7 and 10.3±0.5 weeks). The development of FPI was ascertained to be associated with immune dysfunctions detectable just in early pregnancy as lower natural regulatory CD4+CD25+ and CD4+CD25highT cells and impaired cytokine balance due to enhanced TNF-б along with the decreased production of IL-10 and IL-13 (higher TNF-б/IL-10 and TNF-б/IL-13 ratios), as well as the reduced suppressor activity of serum factors. These immunological parameters may be used as additional criteria in the early prediction of FPI development.

About the authors

A A Ostanin

Research Institute of Clinical Immunology, Siberian Branch of the Russian Academy of Medical Sciences

Email: ct_lab@mail.ru
Research Institute of Clinical Immunology, Siberian Branch of the Russian Academy of Medical Sciences

S M Kustov

Maternity Hospital Seven, Novosibirsk

Maternity Hospital Seven, Novosibirsk

T V Tyrinova

Research Institute of Clinical Immunology, Siberian Branch of the Russian Academy of Medical Sciences

Email: ct_lab@mail.ru
Research Institute of Clinical Immunology, Siberian Branch of the Russian Academy of Medical Sciences

M A Tikhonova

Research Institute of Clinical Immunology, Siberian Branch of the Russian Academy of Medical Sciences

Email: ct_lab@mail.ru
Research Institute of Clinical Immunology, Siberian Branch of the Russian Academy of Medical Sciences

N A Khonina

Research Institute of Clinical Immunology, Siberian Branch of the Russian Academy of Medical Sciences

Email: ct_lab@mail.ru
Research Institute of Clinical Immunology, Siberian Branch of the Russian Academy of Medical Sciences

N M Pasman

Research Institute of Clinical Immunology, Siberian Branch of the Russian Academy of Medical Sciences

Email: ct_lab@mail.ru
Research Institute of Clinical Immunology, Siberian Branch of the Russian Academy of Medical Sciences

Ye R Chernykh

Research Institute of Clinical Immunology, Siberian Branch of the Russian Academy of Medical Sciences

Email: ct_lab@mail.ru
Research Institute of Clinical Immunology, Siberian Branch of the Russian Academy of Medical Sciences

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