Balance of serum cytokines regulating inflammatory activity in patients with leiomyoma

封面

如何引用文章

全文:

开放存取 开放存取
受限制的访问 ##reader.subscriptionAccessGranted##
受限制的访问 订阅或者付费存取

详细

Objective: To analyze the concentrations and balance of serum cytokines that activate (IL-1β, IL-6) and inhibit (IL-4, IL-10) inflammation in healthy individuals and in patients with leiomyoma in general, with and without endometrial lesions.

Materials and methods: The study included 109 women aged 23 to 61 years with uterine fibroids and 92 women of similar age (22–61 years) without uterine fibroids. All women had general clinical, standard instrumental and laboratory examinations. The concentrations of pro-inflammatory and anti-inflammatory cytokines were determined by enzyme-linked immunosorbent assay (ELISA) for ELx800 Plates (BioTek, Taiwan) using Vector-Best (Russia) kits. Descriptive statistical analysis and nonparametric statistical methods were used.

Results: The course of the tumor process in uterine fibroids is accompanied by a fivefold increase in pro-inflammatory cytokines IL-1β and IL-6 in the serum of female patients in comparison with healthy individuals (p<0.001); the concentrations of cytokines with anti-inflammatory activity, namely IL-4, are not so remarkable, but they are also higher (4.09(2.36-6.94) versus 2.42(0.97-5.86) in healthy individuals, p<0.001). Significant increase in IL-6 concentration in all patients with uterine fibroids (8.93(4.95-14.11), p<0.001) was found to be maximal among women with endometrial pathology (9.67(5.22-14.60), p<0.05).

Conclusion: Proliferation of tumor myocytes and development of microcirculatory channel components in patients with uterine fibroids are accompanied by the prevalence of pro-inflammatory activity of the cytokine network and a shift in the balance of its functional state towards dysregulation of control over the processes of myometrial remodeling.

全文:

受限制的访问

作者简介

Vladimir Konenkov

Research Institute of Clinical and Experimental Lymphology, Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences

编辑信件的主要联系方式.
Email: vikonenkov@gmail.com
ORCID iD: 0000-0001-7385-6270

Dr. Med. Sci., Professor, Academician of the RAS, Head of the Clinical Immunogenetics Laboratory

俄罗斯联邦, Novosibirsk

Alla Shevchenko

Research Institute of Clinical and Experimental Lymphology, Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences

Email: shalla64@mail.ru
ORCID iD: 0000-0001-5898-950X

Dr. Biol. Sci., Leading Researcher at the Clinical Immunogenetics Laboratory

俄罗斯联邦, Novosibirsk

Elena Koroleva

Novosibirsk State Medical University, Ministry of Health of Russia

Email: korlex71@mail.ru
ORCID iD: 0000-0002-8522-4382

gynecologist, Junior Researcher, Laboratory of Cellular Biology and Fundamental Basis of Reproduction of Central Scientific Laboratory

俄罗斯联邦, Novosibirsk

Viktor Prokofiev

Research Institute of Clinical and Experimental Lymphology, Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences

Email: vf_prok@mail.ru
ORCID iD: 0000-0001-7290-1631

PhD, Leading Researcher at the Clinical Immunogenetics Laboratory

俄罗斯联邦, Novosibirsk

Yulia Timofeeva

Novosibirsk State Medical University, Ministry of Health of Russia

Email: dr.j.timofeeva@yandex.ru
ORCID iD: 0000-0002-5379-9296

PhD, Assistant at the Obstetrics and Gynecology Department

俄罗斯联邦, Novosibirsk

Svetlana Aidagulova

Novosibirsk State Medical University, Ministry of Health of Russia

Email: asvetvlad@yandex.ru
ORCID iD: 0000-0001-7124-1969

Dr. Biol. Sci., Professor, Head of the Laboratory of Cellular Biology and Fundamental Basis of Reproduction of Central Scientific Laboratory

俄罗斯联邦, Novosibirsk

Igor Marinkin

Novosibirsk State Medical University, Ministry of Health of Russia

Email: rector@ngmu.ru
ORCID iD: 0000-0002-9409-4823

Dr. Med. Sci., Professor, Head of the Obstetrics and Gynecology Department, Rector

俄罗斯联邦, Novosibirsk

参考

  1. Министерство здравоохранения Российской Федерации. Клинические рекомендации. Миома матки. 2020. [Ministry of Health of the Russian Federation. Clinical guidelines. Uterine fibroids. 2020. (in Russian)].
  2. Dudley A.C., Griffioen A.W. Pathological angiogenesis: mechanisms and therapeutic strategies. Angiogenesis. 2023; 26(3): 313-47. https://dx.doi.org/ 10.1007/s10456-023-09876-7.
  3. Mlodawska O.W., Saini P., Parker J.B., Wei J.J., Bulun S.E., Simon M.A. et al. Epigenomic and enhancer dysregulation in uterine leiomyomas. Hum. Reprod. Update. 2022; 28(4): 518-47. https://dx.doi.org/10.1093/humupd/dmac008
  4. Koltsova A.S., Efimova O.A., Pendina A.A. A view on uterine leiomyoma genesis through the prism of genetic, epigenetic and cellular heterogeneity. Int. J. Mol. Sci. 2023; 24(6): 5752. https://dx.doi.org/10.3390/ijms24065752.
  5. Liu C., Chu D., Kalantar-Zadeh K., George J., Young H.A., Liu G. Cytokines: from clinical significance to quantification. Adv. Sci. (Weinh). 2021; 8(15): e2004433. https://dx.doi.org/10.1002/advs.202004433.
  6. Fajgenbaum D.C., June C.H. Cytokine storm. N. Engl. J. Med. 2020; 383(23): 2255-73. https://dx.doi.org/10.1056/NEJMra2026131.
  7. Weyand C.M., Watanabe R., Zhang H., Akiyama M., Berry G.J., Goronzy J.J. Cytokines, growth factors and proteases in medium and large vessel vasculitis. Clin. Immunol. 2019; 206: 33-41. https://dx.doi.org/10.1016/j.clim.2019.02.007.
  8. Коненков В.И., Королева Е.Г., Орлов Н.Б., Прокофьев В.Ф., Шевченко А.В., Новиков А.М., Дергачева Т.И. Уровни провоспалительных цитокинов (IL-1β, IL-6, TNFα, IL-8, IL-12p70, IFNγ) в сыворотке крови с миомой матки. Бюллетень экспериментальной биологии и медицины. 2018; 165(5): 648-52. [Konenkov V.I., Koroleva E.G., Orlov N.B., Prokof'ev V.F., Shevchenko A.V., Novikov A.M., Dergacheva T.I. Blood serum levels of proinflammatory cytokines (IL-1β, IL-6, TNFα, IL-8, IL-12p70, and IFNγ) in patients with uterine myoma. Bulletin of Experimental Biology and Medicine. 2018; 165(5): 648-52. (in Russian)].
  9. Коненков В.И., Королева Е.Г., Орлов Н.Б., Прокофьев В.Ф., Шевченко А.В., Новиков А.М., Дергачева Т.И., Останин А.А. Противовоспалительная активность цитокинов сыворотки крови (IL-4, IL-10, IL-13) и природного антагониста рецептора IL-1β (IL-1ra) у женщин с миомой матки. Акушерство и гинекология. 2018; 10: 80-5. [Konenkov V.I., Koroleva E.G., Orlov N.B., Prokof'ev V.F., Shevchenko A.V., Novikov A.M., Dergacheva T.I., Ostanin A.A. Anti-inflammatory activity of serum cytokines (IL-4, IL-10, IL-13) and the natural IL-1β receptor antagonist (IL-1RA) in women with uterine myoma. Obstetrics and Gynecology. 2018; (10): 80-5. (in Russian)]. https://dx.doi.org/10.18565/aig.2018.10.80-85.
  10. Коненков В.И., Королева Е.Г., Орлов Н.Б., Прокофьев В.Ф. Шевченко А.В. Новиков А.М. Сывороточные уровни факторов роста гемопоэза и ангиогенеза (IL-5, IL-7, IL-9, FGF-β, G-CSF, VEGF и PDGF) у женщин с миомой матки. Медицинская иммунология. 2018; 20(5): 691-8. [Konenkov V.I., Koroleva E.G., Orlov N.B., Prokofiev V.F., Shevchenko A.V., Novikov A.M. Serum levels of hemopoietic and angiogenesis growth factors (IL-5, IL-7, IL-9, FGF-β, G-CSF, VEGF and PDGF) in women with uterine myoma. Medical Immunology. 2018; 20(5): 691-8. (in Russian)]. https://dx.doi.org/ 10.15789/1563-0625-2018-5-691-698.
  11. Коненков В.И., Королева Е.Г., Орлов Н.Б., Прокофьев В.Ф., Шевченко А.В., Новиков А.М., Дергачева Т.И. Изменения концентраций CCL-хемокинов (MCP-1, MIP-1α, MIP-1β, RANTES и EOTAXIN) в сыворотке крови женщин с миомой матки. Акушерство и гинекология. 2019; 8: 107-11. [Konenkov V.I., Koroleva E.G., Orlov N.B., Prokofev V.F., Shevchenko A.V., Novikov A.M., Dergacheva T.I. Changes in the serum concentrations of CCL chemokines (MCP-1, MIP-1A, MIP-1Β, RANTES and EOTAXIN) in women with uterine myoma. Obstetrics and Gynecology. 2019; (8): 107-11. (in Russian)]. https://dx.doi.org/10.18565/aig.2019.8.107-111.
  12. Хворостухина Н.Ф., Островская А.Е., Новичков Д.А., Степанова Н.Н. Цитокиновый профиль при осложнениях гормонотерапии миомы матки. Медицинская иммунология. 2017; 19(6): 739-48. [Khvorostukhina N.F., Ostrovskaya A.E., Novichkov D.A., Stepanova N.N. Cytokine profile in complications of hormone therapy administered for uterine fibroids. Medical Immunology. 2017; 19(6): 739-48. (in Russian)]. https://dx.doi.org/10.15789/1563-0625-2017-6-739-748.
  13. Стагниева И.В., Симбирцев А.С. Эффективность иммуномодулирующей терапии у больных риносинуситом. Медицинская иммунология. 2015; 17(5): 423-30. [Stagnieva I.V., Simbirtsev A.S. Immunotherapy efficiency in rhinosinusitis patients. Medical Immunology. 2015; 17(5): 423-30. (in Russian)]. https://dx.doi.org/10.15789/1563-0625-2015-5-423-430.
  14. Зорина В.Н., Исакова О.В., Зорина Р.М., Баженова Л.Г., Зорин Н.А. Концентрации иммунорегуляторных белков и некоторых цитокинов в крови женщин при приеме менопаузальной терапии. Медицинская иммунология. 2016; 18(2): 177-82. [Zorina V.N., Isakova O.V., Zorina R.M., Bazhenova L.G., Zorin N.A.Concentrations of immunoregulatory proteins and some cytokines in blood of women during menopausaltherapy. Medical Immunology. 2016; 18(2): 177-82. (in Russian)]. https://dx.doi.org/10.15789/1563-0625-2016-2-177-182.
  15. Zhu H., Hu S., Li Y., Sun Y., Xiong X., Hu X. et al. Interleukins and ischemic stroke. Front. Immunol. 2022; 13: 828447. https://dx.doi.org/10.3389/fimmu.2022.828447.
  16. Uyama N., Tsutsui H., Wu S., Yasuda K., Hatano E., Qin X.Y. et al. Anti-interleukin-6 receptor antibody treatment ameliorates postoperative adhesion formation. Sci. Rep. 2019; 9(1): 17558. https://dx.doi.org/10.1038/ s41598-019-54175-1.
  17. Zimmermann M., Aguilera F.B., Castellucci M., Rossato M., Costa S., Lunardi C. et al. Chromatin remodelling and autocrine TNFα are required for optimal interleukin-6 expression in activated human neutrophils. Nat. Commun. 2015; 6: 6061. https://dx.doi.org/10.1038/ncomms7061.
  18. Ayari S., Abellard A., Carayol M., Guedj É., Gavarry O. A systematic review of exercise modalities that reduce pro-inflammatory cytokines in humans and animals' models with mild cognitive impairment or dementia. Exp. Gerontol. 2023; 175: 112141. https://dx.doi.org/10.1016/j.exger.2023.112141.
  19. Liberale L., Ministrini S., Carbone F., Camici G.G., Montecucco F. Cytokines as therapeutic targets for cardio- and cerebrovascular diseases. Basic Res. Cardiol. 2021; 116(1): 23. https://dx.doi.org/10.1007/s00395-021-00863-x.
  20. Raimondo T.M., Mooney D.J. Anti-inflammatory nanoparticles significantly improve muscle function in a murine model of advanced muscular dystrophy. Sci. Adv. 2021; 7(26): eabh3693. https://dx.doi.org/10.1126/sciadv.abh3693.

补充文件

附件文件
动作
1. JATS XML
下载

版权所有 © Bionika Media, 2024
##common.cookie##