Antibacterial activity of probiotic strains of lactobacilli included in the MRM Nutrition Women’s Probiotic complex (Lactobacillus plantarum LP01 and Lactobacillus PARACASEI LPC09)

Lactobacilli, recognized as probiotics, are regarded as promising instruments, both for the prevention and treatment of vaginal dysbiosis and urinary tract infection. Given the ineffectiveness of therapy and the frequent recurrence of these infections, the use of probiotics has become a progressive solution to this problem.

Objective: To evaluate the antibacterial activity of probiotic strains of lactobacilli included in the MRM Nutrition Women's Probiotic complex (Lactobacillus plantarum LP01 and Lactobacillus paracasei LPC09) against opportunistic bacteria isolated from urogenital clinical materials, and to determine the sensitivity of these lactobacilli to antibacterial drugs.

Materials and methods: Probiotic strains of Lactobacillus plantarum LP01 and Lactobacillus paracasei LPC09 were cultivated and the grown colonies were identified using the MALDI-TOF mass spectrometry method (Bruker, Germany). To study the antagonism between probiotic strains of lactobacilli and opportunistic microorganisms, a collection of 16 clinical isolates of opportunistic microorganisms was made: Escherihia coli (3), Klebsiella pneumoniae (2), Morganella morganii (1), Enterococcus faecalis (3), Enterococcus raffinosus (1), Streptococcus agalactiae (3), Staphylococcus epidermidis (1) and Candida albicans (2). To study the sensitivity of probiotic strains of lactobacilli to antimicrobial drugs, a panel for determining the sensitivity of gram-positive bacteria to antimicrobial drugs "Gram-positive AST" (AutoBio Diagnostics, China) was used.

Results: When co-cultivated with probiotic lactobacilli (Lactobacillus plantarum LP01 and Lactobacillus paracasei LPC09), a decrease in the pH of the medium was noted, and a direct antibacterial effect of these lactobacilli against all tested opportunistic microorganisms was detected. When studying antibiotic resistance, it was marked that both strains of lactobacilli were resistant to antibacterial drugs of the penicillin group, cephalosporins, and fluoroquinolones.

Conclusion: Oral administration of the MRM Nutrition Women's Probiotic complex, containing a combination of Lactobacillus plantarum LP01 and Lactobacillus paracasei LPC09, cranberry extract and D-mannose, has significant antibacterial activity against opportunistic microorganisms isolated from women’s urogenital tract. These lactobacilli strains are resistant to some antibacterial drugs widely used in clinical practice, which may be the ground for taking this probiotic drug together with prescribed antibiotics.