Farmaciya (Pharmacy)Farmaciya (Pharmacy)0367-30142541-9218Russkiy Vrach Publishing House11363610.29296/25419218-2022-02-06Research ArticleEvaluation of the quality and biological activity of the «BioChaga» birch fungus extractUsoltsevaOlga NikolaevnaCoordinator of Scientific researchusolceva@baikal-legend.ruOlennikovDaniil NikolaevichLeading Researcherolennikovdn@mail.ruPotupchikTatiana Vitalievna<p>Associate Professor of the Department of Pharmacology and Clinical Pharmacology with a course</p>usolceva@baikal-legend.ruLimited Liability Company «Sibpribor»Federal state budgetary institution of science of the Buryat scientific center, Siberian branch of the Russian Academy of SciencesFederal state budgetary educational institution of higher education «Krasnoyarskstate medical University named after Professor V.F. Voyno-Yasenetsky» of the Ministry of Health of the Russian Federation15022022712334018112022Copyright © 2022, Russkiy Vrach Publishing House2022<p>Introduction. BioChaga is a natural extract of the birch mushroom chaga, produced according to the author's technology of the Irkutsk company SibPribor LLC under the trademark Baikal Legend . The high quality of BioChaga is due to the combination of environmentally friendly chaga medicinal plant raw materials rich in biologically active substances of the Baikal region and th e unique author's technology of its processing. The result is a fast-soluble BioChaga powder with low density, high biological activity and bioavailability. The purpose of the study: a comprehensive study of the composition and content of BAS, biological activity of the BioChaga extract in connection with the peculiarities of its production technology. Material and methods. To determine the physicochemical properties of BioChaga extract we used the following methods: extraction / gravimetry, spectrophotometry, the qualitative composition was studied using high-performance liquid chromatography. To determine the biological activity of BioChaga , the antiradical activity of the extract to organic and inorganic radicals of natural and artificial origin was determined by spectrometry and coulometry. The antioxidant potential was evaluated using spectrophotometry and fluorimetry to determine the total antioxidant potential and the ability to absorb oxygen radicals. The iron-reducing and iron- chelating potential of BioChaga was determined by spectrophotometry. Results. The physico-chemical characteristics of the BioChaga composition had a high level of compounds characteristic of the chaga: low molecular weight phenolic compounds - 57,001,48 mg/kg, high molecular weight phenolic compounds (melanin complex) - 24,91,2%. The nature of the distribution of fragments of the melanin complex shows a predominant content of o-dioxybenzoyl fragments, which suggests the presence of high biological activity in the BioChaga. It has been shown that the BioChaga has antioxidant, antiradical and iron-reducing activity. In addition, the extract has proven to be a good inactivator of nitric oxide molecules, which indicates its anti-inflammatory properties. Conclusion. BioChaga is a highly active natural complex agent characterized as an effective antioxidant created using a unique author's technology. Its high biological activity indicates the effectiveness of the extract and allows it to be recommended as a means of prevention and protection against oxidative stress</p>Inonotus obliquus«BioChaga»birch mushroom chagaInonotus obliquusproduction technologybiologically active substancebiologically active additive«БиоЧага»березовый гриб чагатехнология производствабиологически активное веществобиологически активная добавка[Струпан Е.А., Колодязная В.С., Струпан О.А. Технология получения экстрактов из дикорастущего растительного сырья, широко применяемого в пищевой промышленности и фитотерапии. Вестник КрасГАУ. 2012; 8: 199-205.][Хасанов В.В., Слижов Ю.Г., Чумаков А.А., Бажина С.В. «Анализ состава и свойств сырья березового гриба чаги Inonotus obliquus (PERS.) PILAT, собранного в пермской области. Химия растительного сырья. 2015; 2: 43-8. DOI: 10.14258/jcprm.201502533][Салова Т.Ю., Громова Н.Ю. Теоретические аспекты получения биологически активных веществ из растительного и животного сырья. Успехи современного естествознания. 2016; 3: 39-43.][Баландайкин М.Э. К вопросу об изучении химической структуры и лечебных свойств Inonotus obliquus (PERS.) Pil. Химия растительного сырья. 2013; 2: 15-22. DOI: 10.14258/jcprm.1302015][Переведенцева Л. Использование дикорастущих грибов в лечебных целях в Пермском крае, Россия. Экология и инженерия. 2013; 2: 236-42.][Змитрович И.В., Денисова Н.П., Баландайкин М.Э. и др. Чага и ее биоактивные комплексы: история и перспективы. Формулы фармации. 2020; 2 (2): 84-93. DOI: 10.17816/phf34803][Ma L., Cen H., Dong P., Lu H. et al. Anti-inflammatory and antitumor activity of extracts and compounds from the fungus Inonotus obliquus. Food Chem. 2013; 139 (1-4): 503-8. DOI: 10.1016/j.foodchem.2013.01.030][Семенова Е.В., Тюменцева В.Р., Козубенко А.А. и др. Биологически активные соединения грибов - источник инноваций в медицине. Современные проблемы науки и образования. 2020; 1. Доступно на: https://science-education. ru/ru/article/view?id=29455.][Сысоева М.А., Хабибрахманова В.Р., Гамаюрова В.С. и др. Исследование золя водных извлечений чаги. XII. Осаждение дисперстной фазы водного извлечения чаги при изменении рН среды. Химия растительного сырья. 2009; 1: 131-5.][Zivkovic L., Bajic V., Topalovic D. et al. Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H2O2-Induced DNA Damage in Human Whole Blood Cells. Oxid Med Cell Longev. 2019; 2019: 5039372. DOI: 10.1155/2019/5039372.][Olennikov D., Tankhaeva L.M., Rokhin A.V. et al. Physicochemical properties and antioxidant activity of melanin fractions from Inonotus obliquus sclerotia. Chemistry of Natural Compounds. 2012; 48 (3). DOI:10.1007/s10600-012-0260-y][Государственный реестр лекарственных средств. https://grls.rosminzdrav.ru/GRLS.aspx][Государственная Фармакопея Российской Федерации. XIV издание. Москва, 2018.][Prieto P., Pineda M., Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem. 1999; 269 (2): 337-41. DOI: 10.1006/abio.1999.4019.][Seyoum, A., Asres, K., El-Fiky, F.K. Structure-Radical Scavenging Activity Relationships of Flavonoids. Phytochemistry, 2006; 67: 2058-70. http://dx.doi.org/10.1016rj.phyto-chem.2006.07.002][Yen G.-C., Su H.-J., Yeh C.-T., Wu C.-H., Duh P.-D. scavenging effects of lotus seed extracts on reactive nitrogen species. 2006; 94 (4): 596-602 DOI: 10.1016/j.foodchem.2004.11.052][Potapovich M.V., Metelitza D.I., Shadyro O.I., Kurchenko V.P. Аntioxidant activity of oxygen-containing aromatic compounds. Appl. Biochem. Microbiol. 2011; 47 (4): 346-55. DOI: 10.1134/S0003683811040144][Zhou B., Guo Z., Xing J., Huang B. Nitric oxide is involved in abscisic acid-induced antioxidant activities in Stylosanthes guianensis. Journal of Experimental Botany. 2005; 56: 3223-8.][Song F.Q., Liu Y., Kong X.S. et al. Progress on understanding the anticancer mechanisms of medicinal mushroom: Inonotus obliquus. Asian Pac J. Cancer Prev. 2013; 14 (3): 1571-8. DOI: 10.7314/apjcp.2013.14.3.1571]