In this work, the possibility of combining the methods of chemical functionalization of a mixture of polychlorinated biphenyls before the formation of a mixture of hydroxy- and methoxy-chlorinated biphenyls and the bacterial transformation of the compounds obtained with the aim of their utilization was investigated. As a result of a 100% conversion, a mixture of compounds identified as methoxy- (30 compounds), hydroxy- (44 compounds) and methoxy (hydroxy) derivatives (47 compounds) was obtained by reacting a mixture of Sovol with MeONa in MeOH and DMSO. PCB congeners. The total content of all hydroxy derivatives was 77.2%. It was established that the strain Rhodococcus wratislaviensis KT112-7 carries out the destruction of 73-93% of this mixture at the initial concentration of 0.25-1.50 g/l. The best result was obtained with a decomposition of 0.1 g/l of a mixture of methoxy- and hydroxy-polychlorobiphenyls (on the 10th day the total absence of the starting compounds). The specific rate of destruction was in direct correlation with the initial concentration of the mixture. It has been shown that during bacterial transformation there is no accumulation of toxic intermediate compounds.