Доклады Академии наукДоклады Академии наук0869-5652The Russian Academy of Sciences1352310.31857/S0869-56524863380-383Research ArticlePeroxide-induced oxidative modification of hemoglobinVasilyevaA. D.ms.kadaver@mail.ruYurinaL. V.ms.kadaver@mail.ruBugrovaA. E.ms.kadaver@mail.ruIndeykinaM. I.ms.kadaver@mail.ruAzarovaD. Y.ms.kadaver@mail.ruBychkovaA. V.ms.kadaver@mail.ruAkzhigitovaK. I.ms.kadaver@mail.ruKononikhinA. S.ms.kadaver@mail.ruNikolaevE. N.<p>Corresponding Member of the Russian Academy of Sciences</p>ms.kadaver@mail.ruRosenfeldM. A.ms.kadaver@mail.ruInstitute of Biochemical Physics of the Russian Academy of SciencesMoscow Institute of Physics and TechnologyMoscow State Academy of Veterinary Medicine and Biotechnology named after K.I. SkryabinBelinsky pedagogical Institute of Penza State UniversityTalrose Institute for EnergyProblems of Chemical Physics of the Russian Academy of SciencesSkolkovo Institute of Science and Technology3005201948633803831806201918062019Copyright © 2019, Russian academy of sciences2019<p>The oxidative modification of human hemoglobin Hb treated with ydrogen peroxide was investigated. The method of mass spectrometry were detected oxidized amino acid residues of the hemoglobin molecule: Trp14, Tyr24, Arg31, Met32, Tyr42, His45, His72, Met76, Pro77, Lys90, Cys104, Tyr140, His2, Trp15, Trp37, Met55, Cys93, Cys112, Tyr130, Lys144, His146. The antioxidant potential of the Hb molecule in the intracellular space and when it enters the blood plasma is discussed.</p>hemoglobinmass spectrometryhydrogen peroxideoxidative modificationsгемоглобинмасс-спектроскопияперекись водородаокислительные модификации[Grune T. // Biogerontology. 2000. V. 1. P. 31-40.][Groitl B., Jakob U. // Biochim. Biophys. Acta. 2014. V. 1844. P. 1335-1343.][Rifkind J.M, Mohanty J.G, Nagababu E. // Front Physiol. 2015. V. 5. P. 1-9. doi: 10.3389/fphys.2014.00500. eCollection 2014][Galetskiy D., Lohscheider J. N., Kononikhin A.S., Popov I. A., Nikolaev E. N., Adamska I. // Rapid Commun. Mass Spectrom. 2011. V. 25. P. 184-190.][Vasilyeva A.D., Yurina L.V., Indeykina M.I., Bychkova A.V., Bugrova A.E., Biryukova M.I., Kononikhin A.S., Nikolaev E.N., Rosenfeld M.A. // Biochim. Biophys. Acta. 2018. V. 1866. P. 875-874.][Pimenova T., Pereira C.P., Gehrig T., Buehler P.W., Schaer D.J., Zenobi R. // J. Proteome Research. 2010. V. 9. P. 4061-4070.][Deterding L.J., Ramirez D.C., Dubin J.R., Mason R.P., Tomer K.B. // J. Biol. Chem.2004. V. 279. P. 11 600-11 607.][Ramirez D.C., Chen Y.R., Mason R.P. // Free Radic. Biol. Med. 2003. V. 34. P. 830-839.][Mason R.P. //Free Radic. Biol. Med. 2004. V. 36, P. 1214-1223.][Jia Y., Buehler P.W., Boykins R.A., Venable R.M., Alayash A.I. // J. Biol. Chem. 2007. V. 282. P. 4894-4907.][Kim G., Weiss S.J., Levine R.L. // Biochim. Biophys. Acta. 2014. V. 1840. P. 901-905.][Rosenfeld M.A., Vasilyeva A.D, Yurina L.V, Bychkova A.V. // Free Radic. Res. 2018. V. 52. P. 14-38.]