Доклады Академии наукДоклады Академии наук0869-5652The Russian Academy of Sciences1426710.31857/S0869-56524854515-518Research ArticleThe development of reporter system for the investigation of molecular mechanisms of ecdysone responseMazinaM. Yu.nvorobyova@gmail.comKrasnovA. N.nvorobyova@gmail.comGeorgievP. G.<p>Academician of the Russian Academy of Sciences</p>nvorobyova@gmail.comVorobyevaN. E.nvorobyova@gmail.comInstitute of Gene Biology of the Russian Academy of Sciences2205201948545155182006201920062019Copyright © 2019, Russian academy of sciences2019<p>To study the mechanisms of transcriptional regulation, a convenient experimental approach is to use the artificial chimeric constructs, carrying the regulatory elements of interest. In the present work, we describe the creation and characterization of a novel genetic construct, which makes possible to study the transcriptional regulation of the early-late gene of the ecdysone cascade. Using the data of genome-wide experiments, we have isolated the main regulatory region of the <em>hr4 </em>gene, which was successfully used to create a chimeric reporter construct expressing a fluorescent protein upon the treatment with the ecdysone hormone. This reporter system can be used to study the mechanisms of the ecdysone response, both in cell culture and in tissues, at various stages of the Drosophila development.</p>ecdysonedrosophilahormone, transcriptionэкдизондрозофилагормонтранскрипция[Mitchell N., Cranna N., Richardson H., Quinn L. // Development. 2008. V. 135. P. 2707-2716.][Krasnov A. N., Mazina M. Y., Nikolenko J. V., Vorobyeva N. E. // Cell Biosci. 2016. V. 6. P. 15.][Mazina M. Y., Kovalenko E. V., Derevyanko P. K., Nikolenko J. V., Krasnov A. N., Vorobyeva N. E. // Biochim. Biophys. Acta. 2018. V. 1861. P. 178-189.][Antoniewski C., Laval M., Lepesant J.-A. // Insect Biochem. Mol. 1993. V. 23 P. 105-114.][Shlyueva D., Stelzer C., Gerlach D., Yáñez-Cuna J.O., Rath M., Boryń Ł. M., Arnold C. D., Stark A. // Mol. Cell. 2014. V. 54. P. 180-192.][Li X., Zhao X., Fang Y., Jiang X., Duong T., Fan C., Huang C. C., Kain S. R. // J. Biol. Chem. 1998. V. 273. P. 34 970-34 975.]