Доклады Академии наукДоклады Академии наук0869-5652The Russian Academy of Sciences1431910.31857/S0869-56524855625-628Research ArticleNeuroprotective action of amidic neurolipins in models of neurotoxicity on the culture of human neural-like cells SH-SY5YAkimovM. G.akimovmike@gmail.comAshbaA. M.akimovmike@gmail.comFomina-AgeevaE. V.akimovmike@gmail.comGretskayaN. M.akimovmike@gmail.comMyasoedovN. F.<p>Academician of the Russian Academy of Sciences</p>akimovmike@gmail.comBezuglovV. V.akimovmike@gmail.comShemyakin & Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of SciencesInstitute of Molecular Genetics of the Russian Academy of Sciences2305201948556256282306201923062019Copyright © 2019, Russian academy of sciences2019<p class="a"><span lang="EN-US">It was established that in neurodegeneration models in the human neuron-like cell line SH-SY5Y, amide derivatives of arachidonic and docosahexaenoic acids were inactive in experiments with MPP+ and CoCl<sub>2</sub> but protected from H<sub>2</sub>O<sub>2</sub>. The protective activity of neurolipins decreased in the series DHA-DA AA-SER = AA-GLY AA-GABA = AA-EA and was manifested starting from a concentration of 0.5 nM.</span></p>SH-SY5YneurolipinsendocannabinidsneuroprotectionROS, cytotoxicitySH-SY5YнейролипиныэндоканабиноидынейропротекцияАФКцитотоксичность[Pertwee R. G. Endocannabinoids and Their Pharmacological Actions // Handb Exp. Pharmacol. 2015. V. 231. P. 1-37.][Muthian S., Rademacher D. J., Roelke C. T., Gross G. J., Hillard C. J. Anandamide Content is Increased and CB1 Cannabinoid Receptor Blockade is Protective During Transient, Focal Cerebral Ischemia // Neuroscience. 2004. V. 129. P. 743-750.][Sarne Y., Mechoulam R. Cannabinoids: between Neuroprotection and Neurotoxicity // Curr. Drug Targets CNS Neurol. Disord. 2005. V. 4. P. 677-684.][Sun Y., Alexander S. P., Garle M. J., Gibson C. L., Hewitt K., Murphy S. P., Kendall D. A., Bennett A. J. Cannabinoid Activation of PPAR Alpha; A Novel Neuroprotective Mechanism // Brit. J. Pharmacol. 2007. V. 152. P. 734-743.][Cohen-Yeshurun A., Trembovler V., Alexandrovich A., Ryberg E., Greasley P. J., Mechoulam R., Shohami E., Leker R. R. N Arachidonoyl-L Serine is Neuroprotective after Traumatic Brain Injury by Reducing Apoptosis // J. Cereb. Blood Flow Metab. 2011. V. 31. P. 1768-1777.][Bobrov M.Y., Lizhin A. A., Andrianova E. L., Gretskaya N. M., Frumkina L. E., Khaspekov L. G., Bezuglov V. V. Antioxidant and Neuroprotective Properties of N Arachidonoyldopamine // Neurosci. Lett. 2008. V. 431. P. 6-11.][Бобров М.Ю., Лыжин А. А., Андрианова Е. Л., Грецкая Н. М., Зинченко Г. Н., Фрумкина Л. Е., Хаспеков Л. Г., Безуглов В. В. Антиоксидантные и нейропротекторные свойства N-Докозагексаеноилдофамина // БЭБиМ. 2006. Т. 142. № 10. С. 425-427.][Kovalevich J., Langford D. Considerations for the Use of SH-SY5Y Neuroblastoma Cells in Neurobiology // Methods Mol. Biol. 2013. V. 1078. P. 9-21.][Безуглов В.В., Блаженова А. В., Андрианова Е. Л., Акимов М. Г., Бобров М. Ю., Назимов И. В., Кисель М. А., Шарко О. Л., Новиков А. В., Краснов Н. В., Шевченко В. П., Шевченко К. В., Вьюнова Т. В., Мясоедов Н. Ф. Арахидоноиламинокислоты и арахидоноилпептиды: синтез и свойства // Биоорган. химия. 2006. Т. 32. № 3. С. 258-267.][Akimov M. G., Gretskaya N. M., Zinchenko G. N., Bezuglov V. V. Cytotoxicity of Endogenous Lipids N Acyl Dopamines and their Possible Metabolic Derivatives for Human Cancer Cell Lines of Different Histological Origin // Anticancer Res. 2015. V. 35. P. 2657-2661.][Oesch S., Gertsch J. Cannabinoid Receptor Ligands as Potential Anticancer Agents - High Hopes for New Therapies? // J. Pharm. Pharmacol. 2009. V. 61. P. 839-853.][Godlewski G., Offertaler L., Wagner J. A., Kunos G. Receptors for Acylethanolamides-GPR55 and GPR119 // Prostaglandins Other Lipid Mediat. 2009. V. 89. P. 105-111.][McCue J.M., Driscoll W. J., Mueller G. P. In vitro Synthesis of Arachidonoyl Amino Scids by Cytochrome c // Prostaglandins Other Lipid Mediat. 2009. V. 90. P. 42-48.][McCue J.M., Driscoll W. J., Mueller G. P. Cytochrome c Catalyzes the in vitro Synthesis of Arachidonoyl Glycine // Biochem. Biophys. Res. Communs. 2008. V. 365. P. 322-327.][Hu S.S., Bradshaw H. B., Benton V. M., Chen J. S., Huang S. M., Minassi A., Bisogno T., Masuda K., Tan B., Roskoski R., Jr., et al. The Biosynthesis of N Arachidonoyl Dopamine (NADA), a Putative Endocannabinoid and Endovanilloid, via Conjugation of Arachidonic Acid with Dopamine // Prostaglandins Leukot Essent Fatty Acids. 2009. V. 81. P. 291-301.]