Доклады Академии наукДоклады Академии наук0869-5652The Russian Academy of Sciences1432110.31857/S0869-56524855634-637Research ArticleXenobiotic response elements (XRE) from human CYP1A1 gene enhance the hTERT promoter activityShepelevM. V.mshepelev@mail.ruKalinichenkoS. V.mshepelev@mail.ruSaakianE. K.mshepelev@mail.ruKorobkoI. V.mshepelev@mail.ruInstitute of Gene Biology of the Russian Academy of Sciences2305201948556346372306201923062019Copyright © 2019, Russian academy of sciences2019<p>A hybrid 6XRE-hTERT promoter consisting of the hTERT tumor-specific promoter and six copies of the XRE element from the <em>CYP1A1 </em>human gene promoter was created. Using a human lung cancer cells as a model, we showed that XRE elements in the hybrid promoter greatly increase the activity of the hTERT promoter and ensure the reporter gene transcriptional activation in response to the treatment of the cells with the AhR ligand benzo(a)pyrene. However, similar effects were also observed in normal human bronchial epithelial cells HBEpC, which indicates the loss of the tumor-specific activity by the 6XRE-hTERT hybrid promoter. XRE elements can be used for nonspecific transcription enhancement but are unsuitable for the creation of tumor-specific promoters with enhanced activity.</p>xenobiotic response elements (XRE)hTERT promoter6XRE-hTERT hybrid promoterэлементы ответа на ксенобиотики (XRE)промотор hTERTгибридный промотор 6XRE-hTERT[Das S.K., Menezes M. E., Bhatia S., Wang X. Y., Emdad L., Sarkar D., Fisher P. B. // J. Cell Physiol. 2015. V. 230. P. 259-271.][Fujiwara T., Urata Y., Tanaka N. // Curr. Cancer Drug Targets. 2007. V. 7. P. 191-201.][Wirth T., Zender L., Schulte B., Mund B., Plentz R., Rudolph K. L., Manns M., Kubicka S., Kuhnel F. // Cancer Res. 2003. V. 63. P. 3181-3188.][Davis J.J., Wang L., Dong F., Zhang L., Guo W., Teraishi F., Xu K., Ji L., Fang B. // Cancer Gene Ther. 2006. V. 13. P. 720-723.][Shepelev M.V., Kopantzev E. P., Vinogradova T. V., Sverdlov E. D., Korobko I. V. // Oncol. Lett. 2016. V. 12. P. 1204-1210.][Калиниченко С.В., Шепелев M. В., Вихрева П. Н., Коробко И. В. // Acta Naturae. 2017. Т. 9. С. 66-73.][Murray I.A., Patterson A. D., Perdew G. H. // Nat. Rev. Cancer. 2014. V. 14. P. 801-814.][Li S., Pei X., Zhang W., Xie H. Q., Zhao B. // Int. J. Mol. Sci. 2014. V. 15. P. 6475-6487.][Sarkar P., Shiizaki K., Yonemoto J., Sone H. // Int. J. Oncol. 2006. V. 28. P. 43-51.]