Доклады Академии наукДоклады Академии наук0869-5652The Russian Academy of Sciences1622110.31857/S0869-56524881103-107Research ArticleThe antiproliferative action of the “early” protein of papillomavirus HPV16 E2 on testis toumors of mice induced by the injection of Hela cellsSalyaevR. K.<p>Corresponding Member of the Russian Academy of Sciences</p>salyaev@sifibr.irk.ruRekoslavskayaN. I.salyaev@sifibr.irk.ruStolbikovA. S.salyaev@sifibr.irk.ruSiberian Institute of Plant Physiology and Biochemistry of the Siberian Branch of the Russian Academy of SciencesIrkutsk Scientific Center of the Siberian Branch of the Russian Academy of Sciences2309201948811031072309201923092019Copyright © 2019, Russian academy of sciences2019<p>The аntiploliferative effect of the early protein of high-risk oncogenic human papillomavirus HPV16 E2 on mice testis tumors was discovered after the indyction that was happened after the intramuscular injection of cancer HeLa cells suspension. The regression of mice testis tumors was mostly dramatic during first 1-2 days after oral vaccination with HPV16 E2 (500 mg per mice) made in tomato plant expression system that was gradually decreasing to control variant. The typical monolayer of HeLa cells on the cultivation flask bottom was appeared after seeding of testis tissue cells on the DMEM medium they were only for 18% functionally active according to the detection by the staining with nitroblue tetrazolium and trypane blue.</p>“early” protein HPV16 E2HeLa cellsthe induction of testis tumorsregressiontherapeutic vaccine“ранний” белок ВПЧ16 Е2клетки HeLaиндукция опухолей семенниковрегрессиятерапевтическая вакцина[Blakaj D.M., Fernandes-Fuentes N., Chen Z., Hegde R., Fiser A., Burk R. D., Brenowitz M. // Front Biosci. 2013. V. 14. P. 900-917.][Siddiqa A., Leon K.C., James C.D., Bhatti M.F., Roberts S., Parish R.S. // J. of General Virology. 2015. V. 96. № 8. P. 2274-2285.][Alazawi W., Pett M., Strauss S., Moseley R., Gray J., Stanley M., Coleman M. // British J. of Cancer. 2004. V. 91. № 12. P. 2063-2070.][Frattini M.G., Hurst S.D., Lim H.B., Swaminathan S., Lai-mins L.A. // The EMBO J. 1997. V. 16. № 2. P. 318-331.][Desaintes C., Demeret C., Goyat S., Yaniv M., Thierry F. // The EMBO J. 1997. V. 16. № 3. P. 504-514.][Саляев Р.К., Рекославская Н.И., Столбиков А.С. // ДАН. 2018. Т. 482. № 5. С. 601-604.][Саляев Р.К., Рекославская Н.И., Столбиков А.С. // ДАН. 2019. Т. 484. № 4. С. 503-506.][Foresta C., Patassini C., Bertoldo A., Menegazzo M., Francavilla F., Barzon L., Ferlin A. // PLoS ONE. 2011. V. 6. № 3. e15036.]