Доклады Академии наукДоклады Академии наук0869-5652The Russian Academy of Sciences1870210.31857/S0869-56524894424-428Research ArticleAscorbate peroxidase of moss Dicranum scoparium: gene identification, enzyme activityOneleA. O.minibayeva@kibb.knc.ruChasovA. V.minibayeva@kibb.knc.ruTrifonovaT. V.minibayeva@kibb.knc.ruMinibayevaF. V.minibayeva@kibb.knc.ruKazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center of the Russian Academy of SciencesKazan Federal University1012201948944244281512201915122019Copyright © 2019, Russian academy of sciences2019<p>In present work, the <em>APX</em> gene encoding ascorbate peroxidase in the moss <em>Dicranum scoparium</em> was for the first time cloned and sequenced, high homology of <em>APX</em> with ascorbate peroxidase genes of the mosses <em>Grimmia pilifera</em> and <em>Physcomitrella patens</em> was shown. The structure of the protein was characterized using bioinfomatics approach and the activity of the enzyme under abiotic stresses was studied. An increase in the activity of ascorbate peroxidase was detected during desiccation of <em>D</em>.<em> scoparium</em> shoots. When exposed to heat shock, a decrease in the acti-vity of ascorbate peroxidase correlated with a decrease in the expression of <em>APX</em>. Conserved elements, which were found in the structure of ascorbate peroxidase gene and protein, indicate that these sequences are preserved in the plant genome during evolution, in support of the importance of this enzyme in maintaining cellular redox status.</p>moss Dicranum scopariumabiotic stressascorbate peroxidaseidentification of APX genehydroxyl radicalмох Dicranum scopariumабиотический стрессаскорбат пероксидазаидентификация APX генагидроксильный радикал[Foyer C.H. // Environ. Exp. Bot. 2018. V. 154. P. 134-142. https://doi.org/10.1016/j.envexpbot.2018.05.003][Gest N., Gautier H., Stevens R. // J. Exp. Bot. 2013. V. 64. P. 33-53. https://doi.org/10.1093/jxb/ers297][Smirnoff N. // Free Radic. Biol. Med. 2018. V. 122. P. 116-129. https://doi.org/10.1016/j.freeradbiomed.2018.03.033][Onele A.O., Chasov A., Viktorova L., et al. // S. Afr. J. Bot. 2018. V. 119. P. 132-141. https://doi.org/10.1016/j.sajb.2018.08.014][Li X., Zhang D., Li H., et al. // Front. Plant Sci. 2015. V. 6. P. 38. https://doi.org/10.3389/fpls.2015.00038][Song X.H., Sha W., Jin Z.M., et al. // Adv. Intel. Soft. Compu. 2012. V. 134. P. 433-440. https://doi.org/10.1007/978-3-642-27537-1_53][Rushmore T.H., Morton M.R., Pickett C.B.J. // Biol. Chem. 1991. V. 266. P. 11 632-11 639.][Dabrowska G., Kata A., Goc A., et al. // Acta Biol. Crac. 2007. V. 1. P. 7-17.][Jespersen H.M., Kjaersgard I.V.H., Ostergaard L., et al. // Biochem. J. 1997. V. 326. P. 305-310. https://doi.org/10.1042/bj3260305][Celik A., Cullis P.M., Sutcliffe M.J., et al. // Eur. J. Biochem. 2001. V. 268. P. 78-85. https://doi.org/10.1046/j.1432-1327.2001.01851.x][Bursey E.H., Poulos T.L. // Biochemistry. 2000. V. 39. P. 7374-737. https://doi.org/10.1021/ bi000446s][Lubaina A.S., Meenu Krishnan V.G., Murugan K. // Indian J. Plant Sci. 2013. V. 2. P. 12-22.]