Toxicological ReviewToxicological ReviewТоксикологический вестник0869-7922Federal Scientific Center of Hygiene named after F.F. Erisman64117510.36946/0869-7922-2019-4-16-22ArticlesСтатьиNEW DATA ON THE QUESTION OF INFORMATIVENESS OF EXPERIMENTS ON CELL CULTURES FOR ASSESSMENT OF COMPARATIVE AND COMBINED TOXICITY OF METAL OXIDE NANOPARTICLESНОВЫЕ ДАННЫЕ К ВОПРОСУ ОБ ИНФОРМАТИВНОСТИ ЭКСПЕРИМЕНТОВ НА КЛЕТОЧНЫХ КУЛЬТУРАХ ДЛЯ ОЦЕНКИ СРАВНИТЕЛЬНОЙ И КОМБИНИРОВАННОЙ ТОКСИЧНОСТИ МЕТАЛЛООКСИДНЫХ НАНОЧАСТИЦBushuevaT. V.БушуеваТ. В.
Russian Federation

Bushueva Tatiana Victorovna

620014, Ekaterinburg

Бушуева Татьяна Викторовна - кандидат медицинских наук, заведующая Научно-производственным отделом Лабораторно-диагностических технологий.

620014, Екатеринбургbushueva@ymrc.ruMinigalievaI. A.МинигалиеваИ. А.
Russian Federation

Minigalieva Ilzira Amirovna

620014, Ekaterinburg

Минигалиева Ильзира Амировна - кандидат биологических наук, старший научный сотрудник, заведующий лабораторией промышленной токсикологии.

620014, Екатеринбургilzira-minigalieva@yandex.ruPanovV. G.ПановВ. Г.
Russian Federation

 

Panov Vladimir Grigorievich

620990, Ekaterinburg

Панов Владимир Григорьевич - кандидат физико-математических наук, старший научный сотрудник лаборатории математического моделирования в экологии и медицине.

620990, Екатеринбург

vpanov@ecko.uran.ruKuznetsovaA. N.КузнецоваА. Н.
Russian Federation

Kuznetsova Alisa Nikolaevna

620014, Ekaterinburg

Кузнецова Алиса Николаевна - лаборант-исследователь НПО Лабораторно-диагностических технологий.

620014, Екатеринбург

alisa@ymrc.ruNaumovaA. S.НаумоваА. С.
Russian Federation

Naumova Anna Sergeevna

620014, Ekaterinburg

Наумова Анна Сергеевна - лаборант-исследователь НПО Лабораторно-диагностических технологий.

620014, Екатеринбург

naumova@ymrc.ruSutunkovaM. P.CутунковаМ. П.
Russian Federation

Sutunkova Marina Petrovna

620014, Ekaterinburg

Сутункова Марина Петровна - кандидат медицинских наук, заведующий лабораторией токсикологии среды обитания.

620014, Екатеринбург

sutunkova@ymrc.ruShurV. Ya.ШурВ. Я.
Russian Federation

Shur Vladimir Yakovlevich

620000, Ekaterinburg

Шур Владимир Яковлевич - доктор физико-математических наук, профессор, директор Центра коллективного пользования «Современные нанотехнологии».

620000, Екатеринбург

vladimir.shur@urfu.ruShishkinaЕ. V.ШишкинаЕ. В.
Russian Federation

Shishkina Ekaterina Vladimirovna

620000, Ekaterinburg

Шишкина Екатерина Владимировна - кандидат физико-математических наук, старший научный сотрудник Центра коллективного пользования «Современные нанотехнологии».

620000, Екатеринбургekaterina.shishkina@labfer.usu.ruGurviсhV. B.ГурвичВ. Б.
Russian Federation

Gurvich Vladimir Borisovich

620014, Ekaterinburg

Гурвич Владимир Борисович - доктор медицинских наук, директор.

620014, Екатеринбург

gurvich@ymrc.ruKatsnelsonB. A.КацнельсонБ. А.
Russian Federation

Katsnelson Boris Aleksandrovich

620014, Ekaterinburg

Кацнельсон Борис Александрович - доктор медицинских наук, профессор, заслуженный деятель науки РФ, заведующий отделом токсикологии и биопрофилактики.

620014, Екатеринбургbkaznelson@etel.ruMedical Research Center for Prophylaxis and Health Protection in Industrial WorkersФБУН «Екатеринбургский медицинский научный центр профилактики и охраны здоровья рабочих промпредприятий» РоспотребнадзораInstitute of Industrial Ecology, Urals Branch of the Russian Academy of SciencesИнститут промышленной экологии, УрО РАНSchool of Natural Sciences and Mathematics, Ural Federal UniversityИнститут естествознания и математики, Уральский федеральный университет280820194162204112024Copyright ©; 2019, Bushueva T.V., Minigalieva I.A., Panov V.G., Kuznetsova A.N., Naumova A.S., Sutunkova M.P., Shur V.Y., Shishkina Е.V., Gurviсh V.B., Katsnelson B.A.Copyright ©; 2019, Бушуева Т.В., Минигалиева И.А., Панов В.Г., Кузнецова А.Н., Наумова А.С., Cутункова М.П., Шур В.Я., Шишкина Е.В., Гурвич В.Б., Кацнельсон Б.А.2019Bushueva T.V., Minigalieva I.A., Panov V.G., Kuznetsova A.N., Naumova A.S., Sutunkova M.P., Shur V.Y., Shishkina Е.V., Gurviсh V.B., Katsnelson B.A.Бушуева Т.В., Минигалиева И.А., Панов В.Г., Кузнецова А.Н., Наумова А.С., Cутункова М.П., Шур В.Я., Шишкина Е.В., Гурвич В.Б., Кацнельсон Б.А.https://journals.eco-vector.com/0869-7922/article/view/641175

Isolated and combined damaging effects of PbO and CuO nanoparticles have been estimated on an established line of human fibroblasts using three different cytotoxicity indices based on reduction in: (a) the cellular dehydrogenase activity (MTT Assay), (b) the ATP content (Luminescent Cell Viability Assay), (c) the cellular proliferation, viability, spreading, and attachment to substrate evaluated integrally by continuous impedance-based measurement of the Normalized Cell Index. For all these indicators a clear dependence of cell damage on concentration of metal oxide nanoparticles has been demonstrated for both types of metal oxide nanoparticles, which is adequately described by the hyperbolic function, while at the same level of exposure the quantitative characteristics of cytotoxicity of PbO-NPs in comparison with CuO-NPs are similar. The latter was previously observed in the subchronic experiment on rats. The combined in vitro cytotoxicity of nanoparticles has been also described mathematically using the response surface construction methodology and found to be ambiguous, which is also consistent with the conclusions from the experiment on rats with the same nanoparticles.

Изолированное и комбинированное повреждающее действие наночастиц (НЧ) PbO и CuO оценены на стабильной линии человеческих фибробластов с помощью трёх различных показателей цитотоксичности, основанных на снижении (а) дегидрогеназной активности клеток (МТТ-тест); (б) содержания АТФ (тест на жизнеспособность культуры по интенсивности люминесценции); (в) интегральной оценки клеточной пролиферации, распластывания и прикрепления к поверхности по электрическому импедансу («нормализованный клеточный индекс»). По всем этим показателям продемонстрирована для обоих видов метало-оксидных наночастиц чёткая зависимость повреждения клетки от их концентрации, адекватно описываемая гиперболической функцией, в то время как при одном и том же уровне воздействия количественные характеристики цитотоксичности PbO-НЧ в сравнении с CuO-НЧ схожи. Последнее ранее наблюдалось и в субхроническом эксперименте на крысах. Математически описанная с помощью методологии построения поверхности отклика комбинированная цитотоксичность наночастиц in vitro найдена неоднозначной, что также согласуется с выводами из эксперимента на крысах с теми же наночастицами.

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